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Production method of compound multi-microorganism functional bacterium solution

A production method and technology of functional bacteria, applied in the field of brewing, can solve the problems of not being able to give full play to the performance of brewing functional bacteria, the ineffective complementation of enzyme systems and flavor substances, and the low conversion rate of medium, so as to improve the quality and yield of high-quality base wine , Improve the quality of seasoning wine and the output of high-quality base wine, and the effect of prominent base wine aroma

Inactive Publication Date: 2015-04-08
JIANGSU YANGHE BREWERY JOINT STOCK
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The existing functional bacterial liquid production technology is usually single-strain culture, or mixed culture of a small number of strains according to type: on the one hand, a single strain cannot give full play to the performance of multi-micro-co-fermentation of wine-making functional bacteria; on the other hand, the process is cumbersome and equipment utilization Low yield, low medium conversion rate, long production cycle, and the enzyme system and flavor substances between the strains cannot effectively complement each other; the production method of the composite multi-micro functional bacterial liquid, using the principle of stage-by-stage control of the numerical control fermenter equipment, successfully Multi-strains of functional bacteria and multi-strains of functional yeast were compounded to achieve compound culture and co-fermentation of various types of functional bacteria. By making full use of modern fermentation technology and equipment, the enzyme system and flavor of compound multi-microfunctional bacteria were achieved. The effective complementation of substances fully exerts the performance of wine-making functional microorganisms

Method used

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  • Production method of compound multi-microorganism functional bacterium solution

Examples

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Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: According to the following steps to produce composite multi-microfunctional bacterial liquid

[0041] (1) Preparation of bacterial seed solution: firstly, 10 strains of bacterial functional bacteria screened from soft liquor brewing environment were activated on a slant, and secondly, a circle of activated bacteria was cultured in beef extract peptone liquid with a pH value of 6.5 Carry out pure-bred liquid culture in medium (300mL / 500mL), control the shaker speed at 80r / min, temperature 40°C, and cultivate for 20h to obtain pure-bred functional bacterial seed liquid, and the number of bacterial cells per ml is kept at 10 8 A / mL or so;

[0042] (2) Preparation of aroma yeast seed liquid: firstly, 11 strains of yeast functional bacteria screened from the soft liquor brewing environment were activated on a slant, and secondly, a ring of aroma yeast was soaked in wort powder liquid with a pH value of 3.5 Carry out purebred liquid culture in medium (300mL / 500...

Embodiment 2

[0046] Embodiment 2: According to the following steps to produce composite multi-microfunctional bacterial liquid

[0047] (1) Preparation of bacterial seed liquid: first, 5 strains of functional bacteria: Bacillus subtilis (CCTCCAB130016), Bacillus coagulans (CCTCCAB92066), Bacillus licheniformis (CCTCCAB91060), Paenibacillus polymyxa (CGMCC1.2979), circular Bacillus (CCTCCAB207850) was activated on a slant, followed by taking a ring of activated bacteria and carrying out purebred liquid culture in beef extract peptone liquid medium (300mL / 500mL) with a pH value of 7.0. ℃, cultured for 24 hours, to obtain a pure functional bacterial seed solution, and the number of bacteria per milliliter was kept at 10 7 more than one / mL;

[0048] (2) Preparation of the seed liquid of Aromatoyces cerevisiae: firstly, slant the 6 strains of functional yeast: Candida (CCTCCAY91001), Hansenula (CCTCCAY92053, CCTCCAY93047), Pichia pastoris (CCTCCAY201001), Saccharomyces cerevisiae (CCTCCAY20001...

Embodiment 3

[0052] Embodiment 3: according to the following steps to produce composite multi-microfunctional bacterial liquid

[0053] (1) Preparation of bacterial seed liquid: first, 10 strains of functional bacteria were activated on a slant, and secondly, a ring of bacteria after activation was respectively taken to carry out pure-blood liquid culture in beef extract peptone liquid medium (300mL / 500mL) with a pH value of 7.5, Control the rotation speed of the shaker at 100r / min, the temperature at 30°C, and cultivate for 24 hours to obtain a pure functional bacterial seed solution, and the number of bacteria per ml is kept at 10 8 A / mL or so;

[0054] (2) Preparation of Aroma Yeast Seed Solution: Firstly, 11 Aroma Yeast strains were activated on a slant, and then a ring of Aroma Yeast was respectively taken in a liquid culture medium (300mL / 500mL) with a pH value of 5.5 for pure-breeding Aroma Yeast. Cultivation, controlled shaker speed 80r / min, temperature 37°C, cultured for 24 hours...

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Abstract

The invention discloses a production method of a compound multi-microorganism functional bacterium solution, and belongs to the technical field of wine brewing. The production method disclosed by the invention comprises the following steps: firstly, purely culturing various functional microorganisms respectively; secondly, carrying out secondary seed culture on the functional microorganisms and an aroma-producing yeast respectively; and then inoculating a secondary seed solution into each fermentation tank respectively, and producing the multi-microorganism functional bacterium mixed culture solution by regulating a fermentation culture medium and the fermentation conditions in phases. According to the production method disclosed by the invention, through a multi-microorganism mixed culture technology, production efficiency and equipment utilization rate are increased, a microorganism enzyme system as well as the types and contents of flavour substances are enriched, electricity consumption, water consumption and vapour consumption are saved, relative cost is reduced, and economic benefits are improved; the produced multi-microorganism functional bacterium solution is applied to the production of special flavouring liquor and special base liquor, provides an effective support for the production and blending of Yanghe 'soft-taste' wine with a unique style, and meets the production needs of the 'soft-taste' wine.

Description

technical field [0001] The invention relates to a production method of a composite multi-microfunctional bacterial liquid, which belongs to the technical field of brewing. Background technique [0002] The existing functional bacterial liquid production technology is usually single-strain culture, or mixed culture of a small number of strains according to type: on the one hand, a single strain cannot give full play to the performance of multi-micro-co-fermentation of wine-making functional bacteria; on the other hand, the process is cumbersome and equipment utilization Low yield, low medium conversion rate, long production cycle, and the enzyme system and flavor substances between the strains cannot effectively complement each other; the production method of the composite multi-micro functional bacterial liquid, using the principle of stage-by-stage control of the numerical control fermenter equipment, successfully Multi-strains of functional bacteria and multi-strains of fu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12G3/02C12R1/125C12R1/10C12R1/12C12R1/07C12R1/72C12R1/78C12R1/84C12R1/865
CPCC12N1/20C12G3/02C12N1/16C12N1/18
Inventor 王耀周新虎陈翔陆其刚张聪芝张春林李浩
Owner JIANGSU YANGHE BREWERY JOINT STOCK
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