Novel stevioside production process for reducing melanogenesis in process of soaking stevia rebaudian

A technology of stevia and stevia leaves, which is applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., can solve the problems of large pollution, long production process routes and high energy consumption, and achieves short process routes and good economic benefits. , the effect of low energy consumption

Inactive Publication Date: 2015-04-22
天津北洋百川生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the traditional production process of stevioside, the color of the extract obtained by soaking stevia leaves in water is dark brown, which puts a lot of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061]Take 10kg of stevia leaves, add 200kg of tap water, 3% (6kg) of citric acid, fully mix and stir with a stirring paddle, the stirring speed is 20rpm, the time is 40min, repeat four times, and mix the four extracts. The extract is passed through an ultrafiltration membrane with a molecular weight cutoff of 5KD to remove macromolecular impurities, and then passed through a nanofiltration membrane with a molecular weight cutoff of 1KD to remove small molecular impurities and then concentrated. The concentrated liquid enters the simulated moving bed for continuous adsorption, desorption, desalination and decolorization. The adsorption time was 8 hours, and 85% ethanol-water solution was used for elution (98% absolute ethanol), and the obtained eluate was desalted and decolorized. The sample loading rate during desalting and decolorization is 1.5mL / min. Then alcohol and small molecular impurities are removed through a nanofiltration membrane with a molecular weight cut-off of...

Embodiment 2

[0063] Take 13.6kg stevia leaves, add 200kg tap water, 2% (4Kg) citric acid, soak for 60min, repeat four times. The simulated moving bed adsorption time was 12 hours, and eluted with 60% ethanol-water solution. Other steps are with embodiment 1. The liquid phase measurement results of the mixed extract liquid are: the extraction rate is 90.2%, and the light transmittance is 43.1T%.

Embodiment 3

[0065] Get stevia leaf 10kg, add the citric acid of 200kg tap water, 6% (12Kg), other steps are with embodiment 1. The liquid phase measurement results of the mixed extract liquid are: the extraction rate is 80.3%, and the light transmittance is 35.9T%.

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Abstract

The invention relates to a novel method for producing stevioside. The method comprises the following steps: soaking stevia rebaudian by using citric acid or an aqueous solution of other reducing agents with similar effects of that of the citric acid, soaking for four times, mixing a leaching solution, filtering to remove macromolecular impurities by using an ultrafiltration membrane, removing small molecular weight impurities by virtue of a nanofiltration membrane, concentrating to obtain a concentrated solution, and performing adsorbing, desorbing, desalting and discoloring steps on the concentrated solution by virtue of a multifunctional simulated moving bed separation system; removing alcohol in liquid glucose flowing out of the simulated moving bed by virtue of the nanofiltration membrane, concentrating again, and performing spray drying on the concentrated solution, thereby obtaining high-purity white stevioside powder; and regulating the pH value of the leached stevia rebaudian residues, and extracting chlorogenic acid by taking the leached stevia rebaudian residues as raw materials. According to the method, a redox reaction between polyphenol oxidase and polyphenol substances in the stevia rebaudian can be effectively inhibited, so that the generation of black substances is avoided, the leaching rate of the stevioside is improved, the subsequent workload of removing pigments is greatly reduced, and the whole production method is short in route, low in energy consumption and slight in pollution.

Description

technical field [0001] The invention belongs to the fields of bioengineering and novel sweeteners, and in particular relates to a simple and environmentally friendly new production process of stevioside. Background technique [0002] Stevia is a new type of natural sweetener extracted from stevia leaves, a herb of Compositae, and South America has used stevia as a medicinal herb and sugar substitute for hundreds of years. It has the characteristics of high sweetness and low calorie. Its sweetness is 200-300 times that of sucrose, and its calorific value is only 1 / 300 of that of sucrose. Information from the international sweetener industry shows that stevia has been widely used in food production in Asia, South America and the United States. China is one of the major producers of stevia in the world. [0003] Among the nine sweet components of stevioside, the relative content of stevioside (ST), rebaudioside A (RA) and rebaudioside C (RC) is relatively high, accounting for...

Claims

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Application Information

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IPC IPC(8): C07H15/256C07H1/08
Inventor 乔长晟吕爱弟楼鹏钟海蛟盖丽丰
Owner 天津北洋百川生物技术有限公司
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