Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Paclitaxel immunogen, anti-paclitaxel specific antibody and paclitaxel detection reagent

A technology of paclitaxel and immunogen, which is applied in the field of paclitaxel immunogen and paclitaxel detection reagents, can solve the problems of not being suitable for large-scale clinical application and failing to meet the needs of clinical detection, so as to facilitate clinical promotion and use, reduce detection costs, and immunogen sex high effect

Inactive Publication Date: 2015-04-22
苏州博源医疗科技有限公司
View PDF2 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the main methods for monitoring paclitaxel plasma concentration at home and abroad are enzyme-linked immunosorbent assay (ELISA), high-performance liquid chromatography (HPLC), reversed-phase high-performance liquid chromatography (RP-HPLC), liquid chromatography-tandem mass spectrometry Traditional methods such as LC-MS / MS, but these methods are not suitable for large-scale clinical application
Although paclitaxel assay kits using latex-enhanced immune turbidimetry have been launched in China, they are far from meeting the growing demand for clinical testing.
At present, there is a lack of paclitaxel detection reagents with good stability, high sensitivity, and strong specificity in the market, especially good-quality automated detection reagents. Therefore, the quality of research and development and production meets clinical requirements, strong practicability, and high cost performance. It can be applied to automatic biochemistry The paclitaxel determination reagent of the analyzer has become a hot spot in the in vitro diagnostic reagent industry at home and abroad

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Paclitaxel immunogen, anti-paclitaxel specific antibody and paclitaxel detection reagent
  • Paclitaxel immunogen, anti-paclitaxel specific antibody and paclitaxel detection reagent
  • Paclitaxel immunogen, anti-paclitaxel specific antibody and paclitaxel detection reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1: Synthesis and quantitative detection of paclitaxel derivatives

[0069] The chemical structure of paclitaxel derivatives is shown in formula (IV):

[0070]

[0071] The synthetic route and preparation steps of the above-mentioned taxol derivatives are as follows:

[0072]

[0073] 1. Weigh 1g paclitaxel and 2g succinic anhydride into the reaction system, and then incubate them with 20ml pyridine or dry benzene in a glass flask with continuous reflux;

[0074] 2. Continue heating the reaction mixture at a reflux temperature of 70-80°C for 6 hours, then slowly cool the reaction mixture to room temperature, and gently pour off excess pyridine or benzene;

[0075] 3. The remaining organic components are continuously blown into a nitrogen stream under negative pressure to evaporate and dry, and the dried residual product obtained is the paclitaxel derivative;

[0076] 4. Wash the product obtained above with 60% ethanol prepared with distilled water for more than 10 times to ...

Embodiment 2

[0078] Example 2 Synthesis of Taxol Immunogen

[0079] Paclitaxel immunogen consists of bovine serum albumin (Bovine Serum Albumin, BSA) and the paclitaxel derivative represented by formula (II) -CO-(CH 2 ) n -COO- group is connected. In this embodiment, n=2 is taken as an example to illustrate the method for synthesizing the immunogen in detail. The specific steps are as follows:

[0080] 1. Dissolve 200mg of bovine serum albumin in 50ml of 0.2M, pH 8.5 phosphate buffer;

[0081] 2. Add the following chemicals to a small beaker and stir to dissolve: 200mg synthetic paclitaxel derivative, 3.5ml dimethylformamide, 3.5ml ethanol, 7.0ml 10mM potassium phosphate buffer pH 5.0, 200mg 1-ethyl -3-(-3-Dimethylaminopropyl)carbodiimide, 50mg N-hydroxysulfosuccinimide, stir and dissolve these chemicals at room temperature for 30min;

[0082] 3. Add the dissolved solution dropwise to the BSA solution and stir overnight at 2-8°C to obtain the antigen; the synthesized antigen is purified by dialysi...

Embodiment 3

[0083] Example 3: Preparation of specific antibodies against paclitaxel

[0084] The paclitaxel immunogen prepared in Example 2 was inoculated with experimental animal rabbits by conventional methods, and antiserum was obtained after booster immunization. The specific steps are as follows:

[0085] 1. Dilute the synthesized paclitaxel immunogen to 1.0 mg / ml with PBS to obtain an antigen solution, then mix 1.0 ml of the antigen solution with Freund's complete adjuvant to inject the experimental animal rabbit.

[0086] After 2.2 to 3 weeks, 1.0 ml of the same antigen solution and Freund's incomplete adjuvant were used to inject the above-mentioned experimental animal rabbits once, and then once every four weeks, for a total of 4 injections.

[0087] 3. Take blood from the experimental animal rabbit in step 2, and separate and purify to obtain the anti-paclitaxel specific antibody with a titer of 1:30000-1:50000.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a paclitaxel immunogen and a synthetic method of the paclitaxel immunogen as well as an anti-paclitaxel specific antibody and a detection reagent obtained by the paclitaxel immunogen and a preparation method thereof. The paclitaxel immunogen prepared by the invention is high in immunogenicity, can be induced to obtain a high titer anti-paclitaxel specific antibody and is free of any cross reactions with 62 common medicines. The paclitaxel detection reagent prepared by the antibody can be used for precisely and quickly determining the content of paclitaxel in a sample. Compared with existing detection reagents in the market, the detection reagent has the advantages of being simple and convenient to operate, high in sensitivity, strong in specificity, accurate in result and the like and can be used for effectively lowering the paclitaxel detection cost, thereby facilitating clinical large-scaled popularization and use.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and relates to paclitaxel immunogen, anti-paclitaxel specific antibody and paclitaxel detection reagent. Background technique [0002] Paclitaxel (Paclitaxel), its structural formula is as shown in formula (Ⅲ): [0003] [0004] Paclitaxel is a tricyclic diterpene alkaloid drug extracted from the plant yew (also known as yew). It has a unique anti-tumor effect of inducing tubulin polymerization and inhibiting cell division. It is currently used for the treatment of breast cancer and uterine cancer. , Ovarian cancer and non-small cell lung cancer and other cancer clinical first-line drugs. However, paclitaxel has side effects such as decreased bone marrow density, allergic reactions, neutropenia, hypotension, bradycardia, nausea and vomiting, and its therapeutic effect is highly correlated with its plasma concentration. Therefore, by monitoring the concentration level of paclitaxel in the patient's body, t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/765C07K14/795C07K14/47C07K16/44G01N33/577G01N33/535
CPCC07K19/00C07K14/4717C07K14/765C07K14/795C07K16/06G01N33/535G01N33/577G01N33/94
Inventor 虞留明张华涂建成李冬唐燕
Owner 苏州博源医疗科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com