Method for producing amino acid fertilizer from soybean meal through solid-state fermentation
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A solid-state fermentation and amino acid technology, applied in the direction of nitrogen fertilizer, to achieve the effect of improving the soil environment
Inactive Publication Date: 2015-04-29
OCEAN UNIV OF CHINA
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Retrieval of relevant materials including Chinese patents shows that at present, most root irrigation or foliar fertilizers use soybean plant protein as raw materials, such as mostly products obtained by using catalysts, and degrade them through microbial solid-state fermentation. Macromolecular proteins become water-soluble amino acids or small peptides, and fermentation makes them contain a large number of bacteria that can improve the soil environment and have disease resistance, and there are no reports about using them as fertilizers, especially plant irrigation or foliar fertilizers
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Embodiment 1
[0014] 1000g of soybean meal was used as solid fermentation base; 10g of glucose, 2g of beef extract, KH 2 PO 4 1g, NaCl 1g, MgSO 4 1g, FeSO 4 Mix 0.1g with water to make 1000g nutritional supplement, and adjust the pH value to 7.0 with 1M NaOH aqueous solution; mix the above-mentioned soybean meal solid fermentation base material into the nutritional supplement, and mix the two evenly according to the weight ratio of 1:1 to make a solid fermented Put the culture medium into a polyethylene plastic bag, tie the mouth of the bag tightly, cook at 100°C for 8 minutes, and cool down.
[0015] When inoculating, first prepare actinomycetes seeds: use Gao’s No. 1 medium for the first-level slant, and cultivate it at 32°C for 4 days, and inoculate the second-level slant. . Then prepare Bacillus seed liquid: use nutrient agar for the primary slant, culture at 32°C for 36 hours, inoculate the secondary nutrient broth liquid medium, shake and culture at 32°C for 36 hours to logarithm...
Embodiment 2
[0020] Mix 900g of soybean meal with 100g of bran as a solid fermentation base material; in addition, 10g of glucose, 2g of beef extract, KH 2 PO 4 1g, NaCl 1g, MgSO 4 1g, FeSO 4 Mix 0.1g with water to make 1000g nutritional supplement, adjust the pH value to 7.0 with 1M NaOH aqueous solution; mix the above solid fermentation base material into the nutritional supplement, and mix the two evenly according to the weight ratio of 1:1.5 to make a solid fermentation culture Base, put it into a polyethylene plastic bag, tie the mouth of the bag tightly, cook at 100°C for 8 minutes, and cool.
[0021] When inoculating, first prepare actinomycetes seeds: use Gao’s No. 1 medium for the first-level slant, and cultivate it at 32°C for 4 days, and inoculate the second-level slant. . Then prepare Bacillus seed solution: use nutrient agar for the primary slant, culture it at 32°C for 36 hours, inoculate the secondary nutrient broth liquid medium, and shake it on a shaker at 32°C for ...
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Abstract
The invention relates to a method for producing amino acid fertilizer from soybean meal through solid-state fermentation comprises the following processing steps: uniformly mixing soybean meal with nutritional supplements in a ratio of 1:(0.8-1.5), wherein initial PH is 7.0-7.5; sterilizing, inoculating bacillus and actinomycetes to form complex microbial inoculants, and carrying out solid-state fermentation, wherein inoculum sizes of bacillus and actinomycetes are 5-10% and 4-10% respectively, and turning over 1-2 times everyday during fermentation; stopping fermentation after the solid-state fermentation is carried out for 8-12 days, extracting fermented material with water for 0.5-1 hour according to a ratio of material to water of 1:(10-15); after extraction is finished, filtering by virtue of 200-mesh boulting cloth for separating insoluble substances; and concentrating filtrate to a certain concentration, carrying out spray drying and split charging. The method for producing the amino acid fertilizer from the soybean meal through solid-state fermentation has the advantages of mild reaction conditions, complete degradation, no pollution, low energy consumption, low production cost, formation of disease-resistant substances and the like, and the prepared amino acid fertilizer is of a total dissolution type.
Description
technical field [0001] The invention relates to fertilizers, in particular to a method for producing amino acid fertilizers by solid-state fermentation of soybean meal. Background technique [0002] Due to the large-scale use of chemical fertilizers and pesticides by humans all year round, the natural environment has been severely damaged and pollution has become more and more serious. Green agriculture and pollution-free products have received more and more attention. "Green, environmental protection, and health" have become the mainstream of agricultural development. Most of the root irrigation or foliar fertilizers in the prior art have single functions, and chemically synthesized plant growth regulator+inorganic trace elements are used alone; urea is used alone; potassium dihydrogen phosphate or inorganic trace elements are used alone. The disadvantage is that it cannot meet the nutritional balance of crops. [0003] In recent years, plant protein has also begun to be u...
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