A bacterial reverse-inoculation identification method in blood testing

Abstract

Through reverse-inoculation after suspected disease microorganisms are subjected to inoculated culture, different types of the microorganisms are distinguished by utilization of different growth characteristics and colony characteristics for the different types of the microorganisms after bacterial culture, and a suspected disease bacterium is selected and subjected to drug allergy testing so as to provide basis for a certain type of infection for clinicians. The method provided by the invention has advantages of simple experiments, low costs, easy operation and implementation, good repeatability, and the like, and overcomes limitation of traditional clinical examination to some extent.

Description

technical field [0001] The invention relates to the technical field of medical disease microorganism identification, and in particular provides a simple and accurate method for reverse identification of bacteria in blood tests. Background technique [0002] Normal human blood is free of bacteria. When the human body is infected by bacteria, according to the degree of bacterial infection, the diseases caused by blood bacterial infection can be simply divided into bacteremia, sepsis, sepsis and so on. Bacterial infection of the human body generally causes inflammation in a certain part first, such as boils, carbuncles, pneumonia, encephalitis, etc., in the case of reduced resistance. In medicine, the disease caused by bacteria entering the blood circulation from the local inflammation site is called bacteremia. At this time, a small amount of bacteria will often invade the blood. If the person's general condition is good, this small amount of bacteria will be eliminated by v...

AI Technical Summary

Problems solved by technology

However, in actual clinical testing, the collected blood samples are directly incubated and cultured, and some specific pathogenic bacteria often fail to be detected.

Especially in recent years, the incidence of blood infection is on the rise. Many hospitals with better equipment basically use automatic blood bacterial culture equipment for blood bacterial culture. Generally, the positive rate of blood culture is not high enough, and the culture period is long, which is prone to Contamination leads to problems such as erroneous results, which have a huge impact on the selection of drugs in clinical treatment, timely and effective infection control, and the final patient treatment effect