Incomplete antibody screening colloidal gold reagent kit and preparation method thereof

Abstract

The invention discloses an incomplete antibody screening colloidal gold reagent kit and a preparation method thereof. The reagent kit comprises a first erythrocyte membrane antigen gold particle solution, a second erythrocyte membrane antigen gold particle solution, a third erythrocyte membrane antigen gold particle solution and an antihuman globulin solution, wherein an antigen spectrum of each of the first erythrocyte membrane antigen gold particle solution, the second erythrocyte membrane antigen gold particle solution and the third erythrocyte membrane antigen gold particle solution collectively include D, C, E, c, e, Jka, JKb, M, N, S, s, Fya, Fyb, Dia, K, k, Lea, Leb and P1 antigens. The reagent kit is long in storage time and easy to operate, the complicated process such as centrifuging, washing, and incubation is not needed, and a result is clear and easy to judge. The reagent kit also has the advantage of being suitable for large-scale operation and standard operation.

Description

technical field [0001] The invention belongs to the technical field of antibody detection, and in particular relates to an incomplete antibody screening colloidal gold kit and a preparation method thereof. Background technique [0002] With the development of blood transfusion technology, the incidence of immediate hemolytic reaction caused by wrong identification of ABO blood group has been significantly reduced, while delayed hemolytic reaction caused by immune antibody produced by blood transfusion or pregnancy immunization still occurs from time to time. In order to ensure the safety of clinical blood transfusion of patients, antibody screening is required before blood transfusion, so as to detect incomplete antibodies with clinical significance, which is of great significance for clinical safety of blood transfusion. [0003] Clinical significance of incomplete antibody screening: Although the detection rate of irregular antibodies in normal population is 0.3% to 2%, it...

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Problems solved by technology

[0004] There are many screening methods for irregular antibodies. Currently, the main detection methods on the market are: saline method, enzymatic method, anti-human globulin method, and microcolumn gel method. Most of the above methods have the disadvantages of long detection time and inconvenient operation. (Usually 37°C water bath for 30 minutes, then washed with normal saline for 3 times, and then centrifuged to observe

), because screening red blood cells are all used, and the reagents should be stored at 2-8°C. As the storage time prolongs, the activity of red blood cell antigens will decrease and hemolysis will occur, resulting in extremely unstable quality of screening red blood cell reagents, and the validity period not more than 3 months

In addition, there is a relatively similar method, which is the magnetic bead enzyme immunoassay method for blood group antibody screening. Freshly screened red blood cells are prepared into magnetized screening cell reagents. Incubate with alkaline phosphatase-labeled anti-human globulin, add substrate to develop color, and read the value with a microplate reader after termination to judge the result. The process needs to be washed at least 6 times and incubated 3 times, and the detection time is also longer (60 minutes) Above), the operation is complicated (requires repeated incubation and washing), and large-scale auxiliary equipment (microplate reader) is required. The reagents are composed of magnetized screening cell reagents, so they also need to be stored at 2-8 °C, and the validity period is usually not more than 3 month

The defects of the above detection methods greatly limit the application of these methods in actual clinical practice.

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