Application of carbonic anhydrase iv in preparation of lung adenocarcinoma diagnostic preparation
A technology of carbonic anhydrase and lung adenocarcinoma, applied in the field of biomedicine, can solve the problems that the mechanism has yet to be elucidated, the regulation mechanism is complicated and variable, and the influencing factors
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0026] Example 1 Meta-analysis of high-throughput transcriptome data in lung adenocarcinoma
[0027] The GEO (Gene Expression Omnibus) database is developed and maintained by NCBI (National Center for Biotechnology Information). The GEO database is the largest database of gene expression data. SAGE (Serial Analysis of Gene Expression) data, SARST (Serial Analysis of Ribosomal Sequence Tags) data, MS (Mass Spectrometry) data, proteome data and next-generation high-throughput sequencing data (MPSS, massively parallel sequencing technology), etc. The search keywords were ("lung adenocarcinoma" [MeSH Terms] OR "lungadenocarcinoma" [All Fields]) AND "Homo sapiens" [porgn]. Limit the research type to "expression profiling by array" Data sets that meet the following criteria will be included in our research: ①The selected data set must be genome-wide expression mRNA transcriptome data ②These data come from lung adenocarcinoma case groups and controls The biopsy tissue or cultured ce...
Embodiment 2
[0032] Example 2 Lung adenocarcinoma tissue and normal tissue RAMP3 and CA4 gene expression
[0033] 1. Materials and methods
[0034] 1. Materials
[0035] Collect 5 cases of lung adenocarcinoma tissue and 5 cases of normal tissue, group and number them.
[0036] 2. Method
[0037] 2.1 Extraction of total RNA from lung adenocarcinoma tissue and normal tissue
[0038] use Reagent (invitrogen, Cat. No. 15596-018) was used to extract sample RNA, and the experimental operation was carried out according to the product manual. The specific operation was as follows:
[0039] After collecting the samples, freeze them in liquid nitrogen. After taking them out, put the tissue into a pre-cooled mortar for grinding. After the tissue sample is powdered:
[0040] ① Add Trizol and store at room temperature for 5 minutes;
[0041] ② Add 0.2ml of chloroform, vibrate the centrifuge tube vigorously, mix well, and place it at room temperature for 5-10 minutes;
[0042] ③ After 15 minutes...
Embodiment 3
[0066] Example 3 CA4 gene expression in lung adenocarcinoma tissue and paracancerous tissues
[0067] 1. Materials and methods
[0068] 1. Materials
[0069] Non-small cell lung cancer was collected from hospitalized cases from 2005 to 2010. 134 lung adenocarcinoma tissues and adjacent tissues were collected and numbered separately.
[0070] 2. Method
[0071] Refer to the experimental method in Example 2.
[0072] two results
[0073] Among the 116 samples of lung adenocarcinoma, the expression level of CA4 in the cancer tissue was lower than that in the adjacent tissue in 115 samples, and the expression level of CA4 in the cancer tissue was not clearly different from that in the adjacent tissue in only one sample. The ratio is 99%, indicating that the CA4 gene has a good correlation with lung adenocarcinoma, and can be used to prepare preparations for auxiliary diagnosis or prognosis of lung adenocarcinoma.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com