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Method for preparing visual photonic crystal sensor

A technology of photonic crystals and sensing methods, which is applied in nanotechnology for sensing, measurement of color/spectral characteristics, analysis by making materials undergo chemical reactions, etc., can solve the problems of unfavorable rapid and effective detection, expensive equipment, and sensitivity Poor accuracy and other issues

Active Publication Date: 2015-05-20
INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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Problems solved by technology

Among these methods, high-performance liquid chromatography, gas chromatography, and high-efficiency anion-exchange chromatography-pulse amperometric detection have high sensitivity, but the equipment is expensive, the detection cost is high, and the sample processing method is complicated, which is not conducive to rapid and effective detection; The photometric method needs to add a chromogenic reagent, and the sensitivity and accuracy are not good; in view of the complexity of the glucose structure, the optical rotation method is only suitable as an auxiliary detection method; the biosensor method has the advantages of wide linear detection range and high sensitivity, and Low cost and good application prospects

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  • Method for preparing visual photonic crystal sensor
  • Method for preparing visual photonic crystal sensor

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Embodiment approach 1

[0029] Embodiment 1: Preparation of phenylboronic acid group photonic crystal

[0030] Preparation of phenylboronic acid group photonic crystals: (1) After mixing a certain proportion of common functional monomers, suspension polymerization is carried out with 60mg initiator at 80°C and 370rpm for 45min to form monodisperse nanospheres.

[0031] (2) A certain proportion of common functional monomers are mixed (functional monomer methyl methacrylate is 3ml, isopropylacrylamide, 3-acrylamidophenylboronic acid common functional monomers are in a certain proportion), and the initiator is dissolved In 1.5ml of double-distilled water, the reaction was carried out in 27ml of double-distilled water.

[0032] (3) Screen the uniform particle size by gravity centrifugation, discard the supernatant and sediment, collect the emulsion, calculate its mass fraction, and prepare a certain concentration of monodisperse nano-microsphere emulsion.

[0033] (4) Preparation of photonic crystals by vertical...

Embodiment approach 2

[0040] Embodiment 2: Detection of glucose by photonic crystal sensor based on recognition of phenylboronic acid group with glucose

[0041] (1) Put the prepared photonic crystal under weak alkaline conditions (CHES Buffer with pH=8), and record the detection baseline after the fiber optic spectrometer is stable.

[0042] (2) Prepare 1, 5m mol / L glucose solution, and measure glucose from low concentration to high concentration (1-12, 15, 20m mol / L). After each sample addition, wait for 1-3min. After stabilization, record the detection response value ( Figure 5 , Image 6 ).

[0043] (3) Record the change of structural color before and after photonic crystal detection by digital camera (Cannon, Japan) ( Figure 7 ).

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Abstract

The invention discloses a method for rapidly detecting glucose, and belongs to the technical field of detection. The method comprises the following steps: preparing monodisperse nano microspheres with phenylboronic acid group identification elements by utilizing a suspension polymerization method, preparing the nano microspheres into a photonic crystal by utilizing a vertical settlement self-assembly method, and binding the phenylboronic acid group identification elements on the photonic crystal with the testing factor glucose, so as to enable lattice parameters of the photonic crystal to be changed, and finally the structure and the color to be changed, so that a rapid visual photonic crystal sensor detection method for detecting the glucose on site is constructed. The method has the advantages of high sensitivity, fast response, intuitiveness and visibility. The photonic crystal nano microspheres with the identification elements are prepared by utilizing the suspension polymerization method, and the photonic crystal is prepared by utilizing the vertical settlement self-assembly method, so that the detection cost is obviously reduced, the preparation method is simple and easy, and the storage performance, stability and visibility of a sensor have huge potentials in the glucose detection and application in the food industry and medicine industry.

Description

Technical field [0001] The invention relates to a method for preparing a photonic crystal sensor based on a chemical reaction, in particular to a photonic crystal detection technology based on a boronic acid group to recognize sugars such as glucose. Background technique [0002] Glucose (chemical formula C6H12O6), also known as corn dextrose, maize sugar, or even glucose for short, is the most widely distributed and most important monosaccharide in nature. It is a polyhydroxy aldehyde. Pure glucose is colorless crystals with sweet taste but not as sweet as sucrose. It is easily soluble in water, slightly soluble in ethanol, and insoluble in ether. The aqueous solution rotates to the right, so it is also called "dextrose". Glucose has an important position in the field of biology. It is the energy source and metabolic intermediate product of living cells, that is, the main energy supply material of organisms. The heat released by its oxidation reaction is an important source of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/25G01N21/75B82Y15/00
Inventor 高志贤宁保安洪小迪彭媛白家磊
Owner INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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