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A method suitable for batch production of multiple plant tissue culture

A technology of plant tissue culture and mass production, which is applied in the core technology field of rapid multiplication of various plants, can solve the problems of increasing manpower and material resources, different methods of tissue culture and reproduction, and affecting the cost of factory production, so as to reduce manpower and material resources , easy to obtain materials, and easy to operate

Inactive Publication Date: 2016-08-24
INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In each tissue culture research center, the research object will not be a single species, usually two or more main research products, but the suitable explants and tissue culture propagation methods for each plant are different, and it takes a lot of money time and energy to explore efficient and stable tissue culture propagation methods for each plant, which greatly increases the input of manpower and material resources and directly affects the cost of factory production

Method used

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  • A method suitable for batch production of multiple plant tissue culture
  • A method suitable for batch production of multiple plant tissue culture
  • A method suitable for batch production of multiple plant tissue culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1, Tissue Culture Rapid Propagation of Dioscorea scutellariae, Dioscorea chrysanthemum leaves, Artemisia annua and Pollen

[0035] 1. Culture medium preparation and sterilization

[0036]Primary medium: 1.2×MS+6-BA 3.0mg / L+NAA 0.5mg / L+IAA 0.3mg / L+3% sucrose+0.75% agar.

[0037] Proliferation medium: MS+6-BA 0.5mg / L+NAA 0.5mg / L+CCC 1.0mg / L+3% sucrose+0.75% agar. The above medium was sterilized at 121°C for 20 minutes.

[0038] 2. Primary culture

[0039] Take the aseptic leaves of Dioscorea scutellaria, Dioscorea chrysanthemum leaves, Artemisia annua and Pollen, divide them into small pieces according to 0.5-1.5cm×0.5-1.5cm, transfer the leaves into the culture dish with the primary culture medium, and put the leaves on the outside of each culture dish. The number of implants is 10-12, and the culture is carried out under the following culture conditions: about 24°C, 10-12 hours of light per day, and the light intensity is about 1000-2000lx; after about 60 day...

Embodiment 2

[0045] Example 2, Tissue Culture Rapid Propagation of Dioscorea scutellariae, Dioscorea chrysanthemum leaves, Artemisia annua and Pollen

[0046] 1. Culture medium preparation and sterilization

[0047] Primary culture medium: or preferred medium: 1.2×MS+6-BA 4.0mg / L+NAA 0.5mg / L+IAA 0.5mg / L+3% sucrose+0.75% agar.

[0048] Proliferation medium: MS+6-BA 0.2mg / L+NAA 0.8mg / L+CCC 1.0mg / L+3% sucrose+0.75% agar. The above medium was sterilized at 121°C for 20 minutes.

[0049] Get the aseptic leaves of Dioscorea scutellaria, Dioscorea chrysanthemum leaves, Artemisia annua and Pollen, and the basic operation method is consistent with Example 1; the effect is not much different from that of Example 1.

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Abstract

The invention discloses a method suitable for tissue culture mass production of multiple plants. The method comprises the following steps: (1) explant selection, namely selecting aseptic seedlings obtained from the plants by tissue culture as materials to obtain explants; (2) primary culture, namely inoculating stems or blades of the aseptic seedlings obtained in the step (1) to 2.0-5.0mg / L 1.2*MS+6-BA+0.1-1.0mg / L NAA+0.1-0mg / L IAA, and culturing in a culture medium added with saccharose and agar for 25-40 days to form a large quantity of calluses and buds; (3) propagation culture, namely transferring the calluses and the buds obtained by primary culture to 0.2-1.5mg / L MS+6-BA+0.1-1.0mg / L NAA+0.5-5.0mg / L CCC, and culturing on the culture medium added with the saccharose and the agar for 30 days so as to obtain calluses, buds and roots synchronously; and (4) test seedling transplanting, namely dividing seedlings with roots and leaves into individual plants, and then transplanting so as to achieve the aim of providing a large quantity of high-quality seedlings of the multiple plants timely for the scale development by mass seedling propagation indoors.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a core technology suitable for rapid propagation of various plants. Background technique [0002] Dioscorea plant (Dioscorea) has important medicinal value, and its rhizome saponin is currently used in more than 300 steroidal drugs such as synthetic contraceptives, analgesic and anti-inflammatory drugs, anesthetics, adrenal cortex hormones, sex hormones, and anabolic hormones. The starting raw material of ginseng is called "medicinal gold" by the medical field. The starch in the yam tuber is the raw material for the production of industrial alcohol, and it is also a good bioenergy. Dioscorea is a perennial vine, mainly distributed in tropical and subtropical regions of Asia (China, India, Myanmar) and America (Mexico, the United States, Brazil), especially in China and Mexico. According to records, there are about 650 species of Dioscoreaceae plants in the world, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 黄丽芳夏新界杨宇晨
Owner INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI