Culture medium and culture method for producing gentamicin by virtue of micromonospora fermentation

Abstract

The invention relates to a culture medium and a culture method for producing gentamicin by virtue of micromonospora fermentation. The culture medium comprises a primary seed culture medium, a secondary seed culture medium and a fermentation culture medium. The culture medium is characterized in that the primary seed culture medium and the secondary seed culture medium comprise rice vinasse and low-temperature pressed soybean cake powder; the fermentation culture medium comprises the rice vinasse, the low-temperature pressed soybean cake powder, a weakly acidic cation-exchange resin and a non-ionic surface active agent. According to the culture medium disclosed by the invention, the problem of the costs of the raw materials and the auxiliaries is solved; in addition, the influence of the environment on the raw material and auxiliary sources can be reduced to the utmost extent, sufficient supply of the raw materials and the auxiliaries can be ensured, and stable and efficient production of the gentamicin can be realized; meanwhile, the culture medium and the culture method are capable of increasing the fermentation unit.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a culture medium and a culture method for Micromonospora fermentation to produce gentamicin. Background technique [0002] Gentamicin is an aminoglycoside broad-spectrum antibiotic produced by the fermentation of Actinomycetes Micromonospora. It was first discovered by American Weinstein in 1963 and used clinically in 1969. One of the main antibacterial drugs. At present, the domestic use of Micromonospora fermentation to produce gentamicin, the main problems are: [0003] 1 The fermentation production process has not changed much, and the fermentation unit is maintained at about 1500u / ml, resulting in a low fermentation yield. [0004] 2 The quality of animal nitrogen source contained in fermentation medium affects the quality of fermentation broth and fermentation effect. At present, corn gluten meal or fish meal or both are added to the medium for domestic ...

AI Technical Summary

Problems solved by technology

[0003] 1 The fermentation production process has not changed much, and the fermentation unit is maintained at about 1500u / ml, resulting in a low fermentation yield

[0004] 2 The quality of the animal nitrogen source contained in the fermentation medium affects the quality of the fermentation broth and the fermentation effect

At present, corn gluten meal or fish meal or both are added to the medium for domestic fermentation production of gentamicin, resulting in more residual protein in the late stage of fermentation, higher viscosity of the fermentation broth, and reduced extraction yield

[0005] 3 The production cost of gentamicin is relatively high, and the cost of nitrogen source accounts for about 30% of the fermentation cost

Images