Culture medium and culture method for producing gentamicin by virtue of micromonospora fermentation

A fermentation medium and gentamicin technology, applied in the field of fermentation, can solve the problems of affecting the quality of fermentation broth and fermentation effect, high production cost of gentamicin, excess protein, etc., so as to improve the quality and the level of fermentation technology, The effect of improving the level of fermentation technology and lowering the cost of fermentation

Active Publication Date: 2015-05-27
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] 1 The fermentation production process has not changed much, and the fermentation unit is maintained at about 1500u/ml, resulting in a low fermentation yield
[0004] 2 The quality of the animal nitrogen source contained in the fermentation medium affects the quality of the fermentation broth and the fermentation effect
At present, corn gluten meal or fish meal o

Method used

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  • Culture medium and culture method for producing gentamicin by virtue of micromonospora fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] primary seed culture

[0064] at 1m 3 Add 10L of maltose, 20kg of mixed starch, 1L of soybean oil, 25kg of low-temperature pressed soybean cake powder, 15kg of rice distiller's grains, 5kg of corn gluten powder, 2kg of light calcium carbonate, 1kg of ammonium sulfate, and 10g of maltase into the primary seed tank. Firstly, the primary seed culture medium is sterilized, cooled, and pressurized with sterile air, and then under flame protection, the cultured micromonospora mother bottle fermentation liquid is connected to the primary seed tank for cultivation, and the inoculum size is 0.5L. During the primary seed cultivation process, tank pressure is 0.04-0.08MPa; tank temperature is 34-37°C; air flow: 0-10h, 50m 3 / h; 6h~transplantation: 80m 3 / h; stirring speed 60r / min; pH7~8; culture time 50h. After the primary seed cultivation is completed, the cell concentration is 16%; the pH value is 7.1, and there is no other bacterial contamination.

[0065] secondary seed c...

Embodiment 2

[0071] primary seed culture

[0072] at 1m 3 Add 12L of maltose, 22kg of mixed starch, 2L of corn oil, 27kg of low-temperature pressed soybean cake powder, 16kg of rice distiller's grains, 6kg of corn gluten powder, 2.5kg of light calcium carbonate, 2kg of ammonium sulfate, and 15g of maltase into the primary seed tank. Firstly, the primary seed culture medium is sterilized, cooled, and pressurized with sterile air, and then under flame protection, the cultured micromonospora mother bottle fermentation liquid is connected to the primary seed tank for cultivation, and the inoculum size is 0.6L. During the primary seed cultivation process, tank pressure is 0.04-0.08MPa; tank temperature is 34-37°C; air flow: 0-10h, 52m 3 / h; 6h~transplantation: 85m 3 / h; stirring speed 70r / min; pH7~8; culture time 51h. After the primary seed cultivation is over, the cell concentration is 17%; the pH value is 7.3, and there is no other bacteria contamination.

[0073] secondary seed culture ...

Embodiment 3

[0079] primary seed culture

[0080] at 1m 3 Add 15L of maltose, 25kg of mixed starch, 3L of soybean oil, 30kg of low-temperature pressed soybean cake powder, 17kg of rice distiller's grains, 7kg of corn gluten powder, 3kg of light calcium carbonate, 3kg of ammonium sulfate, and 20g of maltase into the primary seed tank. First, sterilize the primary seed culture medium, cool it, and keep the pressure with sterile air, and then, under flame protection, transfer the cultured Micromonospora mother bottle fermentation liquid into the primary seed tank for cultivation, and the inoculum size is 0.7L. During the primary seed cultivation process, tank pressure is 0.04-0.08MPa; tank temperature is 34-37°C; air flow: 0-10h, 55m 3 / h; 6h~transplantation: 90m 3 / h; stirring speed 80r / min; pH7~8; culture time 52h. After the primary seed cultivation is completed, the cell concentration is 20%; the pH value is 7.5, and there is no other bacterial contamination.

[0081] secondary seed c...

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Abstract

The invention relates to a culture medium and a culture method for producing gentamicin by virtue of micromonospora fermentation. The culture medium comprises a primary seed culture medium, a secondary seed culture medium and a fermentation culture medium. The culture medium is characterized in that the primary seed culture medium and the secondary seed culture medium comprise rice vinasse and low-temperature pressed soybean cake powder; the fermentation culture medium comprises the rice vinasse, the low-temperature pressed soybean cake powder, a weakly acidic cation-exchange resin and a non-ionic surface active agent. According to the culture medium disclosed by the invention, the problem of the costs of the raw materials and the auxiliaries is solved; in addition, the influence of the environment on the raw material and auxiliary sources can be reduced to the utmost extent, sufficient supply of the raw materials and the auxiliaries can be ensured, and stable and efficient production of the gentamicin can be realized; meanwhile, the culture medium and the culture method are capable of increasing the fermentation unit.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a culture medium and a culture method for Micromonospora fermentation to produce gentamicin. Background technique [0002] Gentamicin is an aminoglycoside broad-spectrum antibiotic produced by the fermentation of Actinomycetes Micromonospora. It was first discovered by American Weinstein in 1963 and used clinically in 1969. One of the main antibacterial drugs. At present, the domestic use of Micromonospora fermentation to produce gentamicin, the main problems are: [0003] 1 The fermentation production process has not changed much, and the fermentation unit is maintained at about 1500u / ml, resulting in a low fermentation yield. [0004] 2 The quality of animal nitrogen source contained in fermentation medium affects the quality of fermentation broth and fermentation effect. At present, corn gluten meal or fish meal or both are added to the medium for domestic ...

Claims

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Application Information

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IPC IPC(8): C12P19/50C12R1/29
Inventor 任勇
Owner 宁夏泰瑞制药股份有限公司
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