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A bacterial strain that simultaneously utilizes glucose and xylose to produce alcohol fuels and its application

A technology of alcohol fuel and glucose, which is applied in the field of bioengineering to reduce process energy consumption, improve equipment utilization, and improve economic efficiency

Active Publication Date: 2021-07-13
ENERGY RES INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the butanol-producing Clostridium acetotrophs commonly used in production can efficiently metabolize glucose to produce butanol, and can directly use five-carbon sugars such as xylose to produce butanol, but when both glucose and xylose exist in the fermentation broth, its The metabolic process of xylose is affected by glucose, that is, there is a "glucose repression effect", and the xylose metabolism is only started when the glucose is basically exhausted during the fermentation process

Method used

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  • A bacterial strain that simultaneously utilizes glucose and xylose to produce alcohol fuels and its application
  • A bacterial strain that simultaneously utilizes glucose and xylose to produce alcohol fuels and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1: Isolation, screening and identification of a strain of Clostridium beijerinckii capable of synchronously utilizing glucose and xylose to produce butanol and ethanol.

[0028] Step 1: Sampling

[0029] Samples were collected from the sludge of Jinan No. 1 Wastewater Treatment Plant.

[0030] The second step: enrichment culture

[0031] Take 1 g of the collected sample and put it into a 100 mL enrichment medium blue cap bottle, heat shock it at 80 °C for 10 min, cool it with water, place it in a constant temperature incubator at 37 °C for enrichment culture for 3 days, and select the culture with a large number of bubbles. The solution was further enriched in the enrichment medium, and after five passages, samples were taken for gas chromatography analysis. The fermentation broth with butanol production was transferred to the separation medium to continue the fermentation culture.

[0032] Enrichment medium (for culture of anaerobic bacteria): 5 g xylose, 10...

Embodiment 2

[0053] Production of butanol by batch fermentation of corn cob hydrolyzate by Clostridium beijerinckii SE-2

[0054] 1. Strains:

[0055]Clostridium beijerinckii SE-2, the applicant deposited the strain in the China Center for Type Culture Collection (CCTCC for short) on August 19, 2014, and its deposit number is: CCTCC No. M2014384.

[0056] 2. Culture medium:

[0057] Corn cob hydrolyzate: total sugar content is 49.2 g / L (including 33.5 g / L glucose and 15.8 g / L xylose)

[0058] Seed medium (TYA medium): glucose 40 g, beef extract 2 g, yeast powder 2 g, peptone 6 g, CH 3 COONH 4 3 g, KH 2 PO 4 0.5 g, MgSO 4 ·7H 2 O 0.2 g, FeSO 4 ·7H 2 O 0.01 g, distilled water 1000 mL, pH 6.5, autoclave at 121 ℃ for 15 min, blow with sterile nitrogen for 5 min to drive out oxygen.

[0059] Butanol basic fermentation medium (MP2): corncob hydrolyzate, K 2 HPO 4 0.5 g, KH 2 PO4 0.5 g, CH 3 COONH 4 2.2 g, MgSO 4 ·7H 2 O 0.2 g, MnSO 4 ·H 2 O 0.01 g, NaCl 0.01 g, FeSO4 7H 2 ...

Embodiment 3

[0068] Production of butanol by batch fermentation of corncob hydrolyzate by Clostridium acetobutylicum ATCC824

[0069] 1. The similarities with Embodiment 2 will not be repeated, and the differences will be further explained.

[0070] 2. Strain: Clostridium acetobutylicum ATCC824, which is a model strain for butanol production, can utilize glucose and xylose, but there is a "glucose repression effect" in the fermentation process, and xylose can only be utilized after glucose is fully utilized.

[0071] 2. After the fermentation, the concentrations of glucose and xylose in the fermentation broth were 0.5 g / L and 4.2 g / L respectively, and the concentrations of butanol and ethanol were 11.03 g / L and 1.68 g / L respectively.

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Abstract

A bacterial strain capable of synchronously utilizing glucose and xylose to produce alcohol fuel and its application. The bacterial strain is Clostridium beijerinckii SE‑2, and the strain preservation number is CCTCC No. M2014384. The present invention obtains a bacterial strain capable of synchronously utilizing glucose and xylose through the screening of butanol and ethanol-producing microorganisms capable of synchronously utilizing glucose and xylose in the natural environment. Glucose and xylose in the core hydrolyzate produce butanol and ethanol. The synchronous utilization of glucose and xylose in lignocellulose hydrolyzate can eliminate the "secondary growth phenomenon" in the fermentation process, shorten the fermentation cycle, improve the substrate utilization efficiency and equipment production intensity in the production process, and then improve the production of butanol and butanol from lignocellulose. Economics of the ethanol industry.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a bacterial strain for synchronously utilizing glucose and xylose in lignocellulose raw material hydrolyzate to produce alcohol fuel and its application. Background technique [0002] Due to the intensification of the greenhouse effect and environmental pressure, especially the continuous depletion of fossil resources, bioenergy and chemicals (such as biobutanol, bioethanol and other alcohols) that use renewable biomass resources as raw materials are receiving more and more attention from various countries. attention. In addition, vigorously developing biomass energy can also provide more employment opportunities and reduce CO 2 emission. The production of alcohols such as butanol and ethanol mainly includes chemical methods and biological methods. The biological method is to convert sugars into butanol and ethanol through microbial fermentation, which is the future dire...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/10C12P7/16C12R1/145
CPCC12P7/10C12P7/16C12N1/205C12R2001/145Y02E50/10
Inventor 张杰张晓东李岩华栋梁许海朋梁晓辉赵玉晓金付强牧辉高旻天
Owner ENERGY RES INST OF SHANDONG ACAD OF SCI
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