Organophosphorus detection method based on LSPR (localized surface plasma resonance)

A plasmon resonance and localized surface technology, applied in measuring devices, material analysis through optical means, instruments, etc., can solve problems such as expensive, not in line with the development direction of integrated low-cost sensors, and complicated metal nanostructure steps , to achieve the effect of simple steps, low miniaturization and low cost

Inactive Publication Date: 2015-06-03
CHENGDU UNIV OF INFORMATION TECH
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Although the currently commonly used metal nanostructure fabrication methods such as chemical synthesis, self-assembly, and electron beam / ion beam direct writing have been extensively studied, the steps to fabricate metal nanostructures are still very complicated and expensive, which is not in line with current integrated methods. The development direction of low-cost sensors

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  • Organophosphorus detection method based on LSPR (localized surface plasma resonance)
  • Organophosphorus detection method based on LSPR (localized surface plasma resonance)
  • Organophosphorus detection method based on LSPR (localized surface plasma resonance)

Examples

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Embodiment 1

[0080] Utilize the detection method of the present invention to detect trichlorfon, an organophosphorus pesticide, and the detection steps are as follows: Figure 4 shown.

[0081] (1) Step 4-I, preparing an enzyme sensor chip: selecting K9 glass 401 with a size of 10mm×20mm×2mm as the chip substrate, cleaning and drying the substrate. The AChE was immobilized by adsorption method, and the immobilized AChE 402 was obtained on the surface of the glass substrate.

[0082] The cleaning process of the glass substrate is carried out according to the following steps:

[0083] First, soak the K9 glass substrate in 1.2 moles per liter (M) of sodium hydroxide solution and 1.2M hydrochloric acid solution for 5 minutes (min), and then wash it with deionized water; then, immerse it in concentrated hydrochloric acid solution, soak 1min, wash with deionized water; finally, soak in ethanol, acetone and deionized water for 5min respectively; blow dry or dry at room temperature.

[0084] Im...

Embodiment 2

[0090] Embodiment 2, using the detection method of the present invention to detect the simulant dimethyl methyl phosphate (DMMP) gas of sarin poisonous gas, the detection steps are as follows Figure 4 shown.

[0091](1) Step 4-I, preparing an enzyme sensor chip: selecting K9 glass 401 with a size of 10mm×20mm×2mm as the chip substrate, cleaning and drying the substrate. The AChE was immobilized by cross-linking method, and the immobilized AChE 402 was obtained on the surface of the glass substrate.

[0092] The cleaning process of the glass substrate is carried out according to the following steps:

[0093] First, soak the K9 glass substrate in 1.2M sodium hydroxide solution and 1.2M hydrochloric acid solution for 5 minutes, and then wash it with deionized water; then, immerse it in concentrated hydrochloric acid solution, soak it for 1 minute, and wash it with deionized water; Finally, soak in ethanol, acetone and deionized water for 5 minutes respectively; dry or dry at r...

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Abstract

The invention relates to the field of biochemical detection, in particular to an organophosphorus detection method based on LSPR (localized surface plasma resonance). According to the method, an organophosphorus sample is sufficiently contacted with an acetyl-cholinesterase immobilized sensing chip, and an organophosphorus inhibiting enzyme film is formed on the surface of the sensing chip; the sensing chip containing the organophosphorus inhibiting enzyme film is immersed into a mixed solution of acetylthiocholine and chloroauric acid for a sufficient reaction, and gold nanoparticles are formed on the surface of the sensing chip; the sensing chip containing the gold nanoparticles is irradiated by visible light or infrared light, and an extinction spectrum of transmission light is measured, and an LSPR spectrum is acquired; the LSPR spectrum is compared with a standard LSPR spectrum, whether the organophosphorus sample contains organophosphorus is determined according to deviation of absorption peak; the concentration of the organophosphorus is determined according to displacement of the absorption peak. Metal nanoparticles are not required to be prepared before the acetyl-cholinesterase film is immobilized, the detection process is simplified, and low cost is realized.

Description

technical field [0001] The invention relates to the field of biochemical detection, in particular to an organic phosphorus detection method based on localized surface plasmon resonance. Background technique [0002] Organophosphorus compounds generally refer to organic gases containing carbon-phosphorus bonds in their chemical formulas or phosphate compounds containing organic functional groups, generally including various organophosphorus pesticides and nerve gases commonly used in biochemical battlefields. Organophosphorus compounds have a strong inhibitory effect on acetylcholinesterase (Acetyl-cholinesterase, AChE) in the organism, which can cause the accumulation of acetylcholine (Acetylcholine, ACh), lead to central nervous system dysfunction, and cause the organism to lose function until death. A highly effective biological agent. With the wide application of organophosphorus compounds in today's society, their negative impact on the environment, biosphere and even...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/552
Inventor 马文英姚尧魏耀华唐雨竹王海时
Owner CHENGDU UNIV OF INFORMATION TECH
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