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Golden nanostars and preparation method and application thereof

A gold nano and nano technology, which is applied in the field of nano material preparation and biomedicine, can solve the problems that are difficult to remove completely, and achieve the effects of good photothermal stability, low energy consumption and simple preparation method

Inactive Publication Date: 2015-06-10
FUZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[J. Phys. Chem. B, 2006, 110, 19291-19294.] Although branched gold nanostars can be synthesized, CTAB needs to be added as a stabilizer in the reaction, and CTAB has a high Cytotoxic, difficult to completely remove in subsequent sample processing

Method used

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  • Golden nanostars and preparation method and application thereof
  • Golden nanostars and preparation method and application thereof
  • Golden nanostars and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0033] (1) Glassware is first soaked in aqua regia (hydrochloric acid: nitric acid volume ratio is 3:1), and then washed with ultrapure water for several times before use (note: aqua regia is highly corrosive, so be careful when using it) . Specific synthesis steps: 80 μL of 20 mg mL -1 The chloroauric acid solution was added to 19.6 mL of ultrapure water and heated to 35 °C under magnetic stirring. After the temperature was constant for about 1 minute, 400 μL of 100 mM gallic acid solution was quickly added. When the reaction started, the color of the solution was light yellow, and after about 50 seconds, the visible solution gradually turned blue-green, and the reaction stopped after 5 minutes. After the reaction product was centrifuged at 9000 rpm for 5 minutes, the precipitate was collected, washed 3 times with ultrapure water, and then ultrasonically dispersed in about 1 ml of ultrapure water.

[0034] (2) In order to make gold nanostars better applied in cell experim...

Embodiment 2

[0038] (1) Glassware is first soaked in aqua regia (hydrochloric acid: nitric acid volume ratio is 3:1), and then washed with ultrapure water for several times before use. Specific synthesis steps: 80 μL of 20 mg·mL -1 The chloroauric acid solution was added to 19.6 mL of ultrapure water and heated to 35 °C under magnetic stirring. After the temperature was constant for about 1 minute, 300 μL of 100 mM tannic acid solution was quickly added. When the reaction started, the color of the solution was light yellow, and after about 50 seconds, the visible solution gradually turned blue-green, and the reaction stopped after 5 minutes. After the reaction product was centrifuged at 9000 rpm for 5 minutes, the precipitate was collected, washed 3 times with ultrapure water, and then ultrasonically dispersed in about 1 ml of ultrapure water.

[0039] (2) In order to make gold nanostars better applied in cell experiments, gold nanostars were modified with chitosan, specifically, 100 μL o...

Embodiment 3

[0042] (1) Test the photothermal performance of gold nanostars under NIR light irradiation: Prepare 1 mL of gold nanostar solutions with different concentrations (0-20 μg·mL -1 ) were respectively taken in constant quartz cuvettes, and the near-infrared laser was used at 808 nm, 2 W·cm -2 Irradiate for 10 minutes. The temperature is measured in real time by immersing a thermocouple probe (0.5mm in diameter) into the solution. The probe should be placed in a position to avoid direct laser irradiation and not touch the cuvette. The photothermal heating curves of gold nanostars with different concentrations are as follows: Figure 4 As shown, the visible gold nanostars can rapidly heat up the aqueous solution under laser irradiation.

[0043] (2) HeLa cells were seeded in a 96-well plate, and after incubation for 24 hours, the culture medium was removed, and gold nanostar solutions containing different concentrations (0-30 μg·mL -1 ) culture solution for another 4 hours, and t...

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Abstract

The invention discloses golden nanostars and a preparation method and application of the golden nanostars. The preparation method includes: taking the chloroauric acid as the gold source and the plant polyphenol as the reducing agent and the stabilizer, and controlling and synthesizing the size, the morphology and the plasma resonance absorption peak of the golden nanostars through the adjustment of the concentration of the chloroauric acid and the plant polyphenol. The preparation method of the golden nanostars is simple, moderate, free of seed crystal and surface active agent, less in time consuming, low in energy consumption, easy to scale and capable of rapidly synthesizing the golden nanostars just by one step of reaction. The synthesized golden nanostars has the performances of strong near-infrared absorption and good photo-thermal conversion and biological compatibility, is high in photo-thermal conversion efficiency, good in near-infrared photo-thermal stability, strong in opto-acoustic imaging capability, applicable to the photo-thermal treatment and opto-acoustic imaging of cancer cells and wide in application prospect for the clinics, especially for the photo-thermal treatment of imaging guidance.

Description

technical field [0001] The invention belongs to the fields of nanomaterial preparation and biomedicine, and in particular relates to a gold nanostar and its preparation method and application. Background technique [0002] In recent years, the development of multifunctional diagnostic and therapeutic nanomaterials is a very active research field in cancer diagnosis and therapy applications. These therapeutic nanoparticles integrate a variety of imaging modalities for imaging-guided drug delivery and minimally invasive treatment, greatly improving the efficiency of diagnosis and treatment and reducing the risk of side effects caused by multiple administrations. Nanomaterials based on a single near-infrared photoresponse can be used for real-time photoresponse imaging (such as photoacoustic imaging, optical imaging) and simultaneous photoresponse therapy (such as photothermal therapy, photodynamic therapy, and photoinduced chemical drug therapy). A promising material for diag...

Claims

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Application Information

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IPC IPC(8): B22F1/00B22F9/24B82Y30/00B82Y40/00
Inventor 杨黄浩张晓龙郑琤梁虹
Owner FUZHOU UNIVERSITY
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