Preparation process for microbial preparation for repairing nitrotoluene contaminated soil
A technology for microbial preparation and polluted soil, applied in the field of microorganisms, can solve the problems of inability to double as fertilizer, poor treatment effect of dinitrotoluene, slow reproduction rate of strains, etc., and achieves the effects of low cost, increased yield, and simple and feasible process
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[0019] Example 1
[0020] A preparation process of a microbial preparation for repairing soil contaminated by nitrotoluene, which includes the following steps: mixing and stirring the compound bacterial liquid and biological organic matter uniformly, culturing at 30°C for 12 hours, and then adding auxiliary materials and natural minerals, 100 rpm / Min stirring for 3 minutes, and finally low-temperature drying, the drying temperature is 20 ℃, the moisture content after drying is 8% (w / w), it is obtained; the mass ratio of the composite bacterial liquid, auxiliary materials, natural minerals and biological organic matter is 1 :1:3:10.
[0021] The compound bacterial liquid is prepared according to the following method:
[0022] Acinetobacter baumannii, Bacillus cereus, Rhodococcus rubrum, Micrococcus luteus, and Enterococcus faecalis were cultured to a concentration of 1×10 8 Cells / m1 of bacterial liquid, mix and stir evenly according to the volume ratio of 1:1:2:3:3 to obtain;
[0023...
Example Embodiment
[0030] Example 2
[0031] A preparation process of a microbial preparation for repairing soil contaminated by nitrotoluene, which includes the following steps: mixing and stirring the compound bacterial liquid and biological organic matter uniformly, culturing at 30°C for 12 hours, and then adding auxiliary materials and natural minerals, 100 rpm / Min stirring for 3 minutes, and finally low-temperature drying, the drying temperature is 20 ℃, and the moisture content after drying is 8% (w / w), which is obtained; the mass ratio of the composite bacterial liquid, auxiliary materials, natural minerals and biological organic matter is 2 :2:4:15;
[0032] The compound bacterial liquid is prepared according to the following method:
[0033] Acinetobacter baumannii, Bacillus cereus, Rhodococcus rubrum, Micrococcus luteus, and Enterococcus faecalis were cultured to a concentration of 1×10 8 Bacteria / ml, mix and stir evenly according to the volume ratio of 1:1:2:3:3 to obtain;
[0034] The Acin...
Example Embodiment
[0045] Example 3
[0046] Test of repairing contaminated soil by the invention
[0047] The microbial preparation prepared by the process of Example 2 of the present invention is used to treat the contaminated soil near an explosive factory. The soil area is selected as 1 mu of land. First, the soil is loosened and leveled, and the depth of the soil is 40cm, and water is sprayed to keep the soil moisture content at 30 %, and then use 200g of microbial preparations per cubic meter of soil to sow the microbial preparations. One week later, detect the content of nitrobenzene, see Table 1:
[0048] Table 1
[0049] Index
[0050] Conclusion: After testing, it is found that the microbial preparation prepared by the process of the present invention can effectively remove nitrotoluene substances.
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