Method for inducing embryonic stem cells to be differentiated into erythroid cells in vitro

A technology of embryonic stem cells and erythroid cells, which is applied in the field of in vitro induction of embryonic stem cells to differentiate into erythroid cells, can solve the problems of poor stability of hemoglobin oxygen carriers, weak oxygen-carrying capacity of fluorocarbons, complex process, etc., and achieve effective cell sources , easy to obtain materials, and easy to amplify in vitro
CN104694471AInactive Publication Date: 2015-06-10奥思达干细胞有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
奥思达干细胞有限公司
Publication Date
2015-06-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an effective method for inducing embryonic stem cells to be differentiated into erythroid cells in vitro. The embryonic stem cells are pre-induced through an inducing system containing dexamethasone, subjected to induction culture through a serum-free medium system containing a serum-free culture additive N2 or B27, prostaglandin E2 and L-glutamine, and then induced to be differentiated into the erythroid cells through a serum-free medium system containing stem cell factors (SCF), interleukin 3 and hemopoietin. The method specifically includes the following steps of conventionally culturing the embryonic stem cells in vitro, pre-inducing the embryonic stem cells, directionally differentiating the pre-induced cells into hematopoietic stem cells, and directionally differentiating the hematopoietic stem cells into the erythroid cells. According to the method for inducing the embryonic stem cells to be differentiated into the erythroid cells in vitro, materials are convenient to obtain, expansion in vitro is facilitated, immunogenicity is low, and therefore the human embryonic stem cells are differentiated into the mature erythroid cells more safely and efficiently, and the technological application prospects are good.
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Description

technical field

[0001] The invention belongs to the field of regenerative medicine and biotechnology, and relates to a preparation method of erythroid cells, in particular to a method for inducing embryonic stem cells to differentiate into erythroid cells in vitro. Background technique

[0002] With the rapid development of my country's medical technology and the increasing requirements of people's lives, component blood transfusion has been deepened in clinical medical practice, among which erythroid cells (also called blood cells) are the most used. Erythrocyte transfusion is a vital cell therapy method in modern medical technology. The main purpose is to improve the hypoxic state of patients. It is widely used in the treatment of postoperative and trauma, chronic anemia and other diseases. However, at present, clinical practice still relies on voluntary blood donation as the main source of blood. The blood supply is very tight, and reports of insufficient blood supply are...

Claims

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