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Arabinogalactan of flowers of panax notoginseng (burK.)F.H.Chen, and preparation method and use thereof

A technology of arabinose and Panax notoginseng is applied in the field of preparing antitumor drugs, which can solve the problems of low toxicity and side effects, high mortality of pancreatic cancer, and difficulty in early clinical diagnosis.

Active Publication Date: 2015-06-17
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The incidence of pancreatic cancer in my country has increased by 4 times in the past 20 years, and the overall 5-year survival rate of pancreatic cancer patients is less than 5%. The mechanism of development of pancreatic cancer is not yet fully understood [see: Kim EJ, Simeone DM. Advance in pancreatic cancer [J]. Curr Opin Gastroenterol, 2011, 275:460-66. and Philp PA, Mooney M, Jaffe D, et al .Consensus report of the national cancer institute clinical trials planning meeting on pancreas cancer treatment[J].Clin Oncol,2009,27(33):5660-5669.]
Although gemcitabine-based chemotherapy is the standard chemotherapy regimen, it has only a slight advantage in improving symptoms and prolonging survival. Therefore, the clinical treatment of pancreatic cancer still needs to develop new drugs, especially those that can inhibit tumor growth and inhibit tumor growth. It transfers, and the drug with low toxic and side effects

Method used

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  • Arabinogalactan of flowers of panax notoginseng (burK.)F.H.Chen, and preparation method and use thereof
  • Arabinogalactan of flowers of panax notoginseng (burK.)F.H.Chen, and preparation method and use thereof
  • Arabinogalactan of flowers of panax notoginseng (burK.)F.H.Chen, and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Preparation of Arabinogalactan RN1

[0029] a. Polysaccharide extraction:

[0030] Dried notoginseng flowers were degreased with 95% ethanol for one week, and then dried naturally at room temperature. 1000 g of the dried notoginseng flower was extracted 5 times with 20 liters of boiling water (deionized water), 6 hours each time. Sulfuric acid-phenol detected no obvious reaction, filtered, combined the extracts each time, heated and concentrated to 2 liters, added three times the volume of 6 liters of 95% ethanol under stirring, stood overnight, poured the supernatant, and centrifuged After separation, the obtained precipitate was washed with 2 times the volume of absolute ethanol, centrifuged, and the precipitate was vacuum-dried at 40° C. to obtain 100 g of crude polysaccharide from Panax notoginseng flower extracted by water.

[0031] b. Polysaccharide purification:

[0032] Take 10 g of the Panax notoginseng crude polysaccharide prepared above, dissolv...

Embodiment 2

[0040] Example 2: Arabinogalactan RN1 inhibits tumor activity of pancreatic cancer

[0041] MTT (tetrazolium salt method) experiment

[0042] In the experiment, human pancreatic cancer cell lines BxPC-3 and AsPC-1 and human normal liver cell line LO2 (Cell Bank of Typical Culture Collection Committee of Chinese Academy of Sciences, Cell Resource Center of Shanghai Institute of Biological Sciences, Chinese Academy of Sciences) were used to culture in 10% fetal bovine serum (Gibco), 100 U / mL penicillin and 100 U / mL streptomycin in DMEM medium (HyClone). Cells at 37°C with 5% CO 2 cultured in an incubator.

[0043] BxPC-3, AsPC-1, LO2 cells in logarithmic growth phase 5×10 3 were inoculated in a 96-well plate, discarded the supernatant after incubation in an incubator for 24 hours, and added 100 μL of a culture medium solution containing uniform polysaccharide RN1 (prepared in Example 1) to make the final concentration 62.5, 125, 250, 500 and 1000 μg / mL , each concentration h...

Embodiment 3

[0046] Example 3: Polysaccharide RN1 inhibits neovascularization and its migration

[0047] In vitro angiogenesis assay

[0048] In the experiment, human epidermal vascular cells HMEC-1 (founded by Emory University in the United States and preserved in the Shanghai Institute of Materia Medica, Chinese Academy of Sciences) were cultured in a medium containing 15% fetal bovine serum (Gibco Company), 2mM L-glutamine, 10ng / mL EGF, 100U / mL penicillin and 100U / mL streptomycin in MCDB131 medium (Gibco). Cells at 37°C with 5% CO 2 cultured in an incubator.

[0049] Add 50 μL of thawed Matrigel at 4°C to a 96-well plate pre-cooled at 4°C, solidify at 37°C for 30 minutes, and then add 4.5×10 HMEC-1 cells containing 0, 0.5 mg / mL and 1 mg / mL RN1 4 were cultured for 12 h in the cell culture incubator. The results were recorded with an inverted microscope at a magnification of 40 times, and the average length of the lumen in the field of view was counted using Image J software.

[0050...

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Abstract

A polysaccharide extracted from sanchi flowers, preparation method thereof and uses in preparing antitumor drugs. Particularly, an Arab galactan extracted from sanchi flowers, the preparation method comprising: first extracting crude polysaccharide from sanchi flowers, then conducting alcohol precipitation, ion column chromatographic purification, and spectrum identification to obtain Arab galactan.

Description

technical field [0001] The present invention relates to polysaccharides, their extraction methods and their use in the preparation of medicines, in particular to a method for extracting arabinogalactan polysaccharide RN1 from flowers of Panax Notoginseng (BurK.) F.H.Chen The method, the arabinogalactan polysaccharide extracted by the method and the application of the arabinogalactan polysaccharide in the preparation of antitumor drugs. Background technique [0002] Pancreatic cancer is a highly malignant digestive system malignancy with insidious onset, rapid progression and poor prognosis. About 80% of patients are diagnosed with local invasion or distant metastasis. The incidence of pancreatic cancer in my country has increased by 4 times in the past 20 years, and the overall 5-year survival rate of pancreatic cancer patients is less than 5%. The mechanism of development of pancreatic cancer is not yet fully understood [see: Kim EJ, Simeone DM. Advance in pancreatic cancer...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P35/04A61P35/00
CPCA61K36/258A61K2236/00A61P35/00A61P35/04C08B37/0003C08B37/0087C08L5/00
Inventor 丁侃王培培张蕾
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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