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Production method for microbial flocculants

A microbial flocculant and production method technology, applied in the direction of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve the problems of poor flocculant flocculation effect and long production method cycle, and achieve good flocculation effect and low price , the effect of short production cycle

Inactive Publication Date: 2015-06-24
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problems that the existing flocculant production method has a long cycle and the flocculant has poor flocculation effect, and provides a production method of microbial flocculant

Method used

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  • Production method for microbial flocculants
  • Production method for microbial flocculants
  • Production method for microbial flocculants

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specific Embodiment approach 1

[0016] Specific embodiment one: the production method of microbial flocculant of the present embodiment, carry out according to the following steps:

[0017] 1. Pick Acinetobacter and inoculate it into the rich medium, and cultivate it for 18h-24h under the conditions of 28°C-35°C, 140r / min-160r / min, and pH 6.5-7.5, to obtain the activated bacterial liquid, and the activated The bacterial solution is inserted into the flocculation medium according to the inoculum amount of 5%;

[0018] 2. Carry out fermentation culture according to the following conditions: the initial pH is 6-8, the culture temperature is 15°C-30°C, the rotating speed of the shaker is 120r / min-150r / min, and the culture time is 20h-30h to obtain the fermentation liquid;

[0019] 3. Centrifuge the fermentation broth at 3000r / min for 10min, collect the bacterial precipitate, wash it with PBS for 2-3 times, then add the bacterial lysate, then perform ultrasonic crushing in ice bath for 20min, and centrifuge the o...

specific Embodiment approach 2

[0033] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the enrichment medium described in step one is made of 0.2g / L MgSO 4 ·7H 2 O, 5g / L glucose, 3.8g / L beef extract, 10g / L peptone, 16g / L agar and distilled water, pH 7.2. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0034] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the flocculation medium described in step one consists of 14~18g / L glucose, 8~12g / L sucrose, 0.5~0.8g / L yeast extract , 0.08~0.15g / L urea, 0.4~0.6g / L ammonium sulfate, 0.8~1.2g / L KH 2 PO 4, 2~3g / L K 2 HPO 4 , 0.2~0.4g / L MgSO 4 , 0.1~0.3g / L NaCl and distilled water. Others are the same as in the first or second embodiment.

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Abstract

The invention relates to a flocculant production method, in particular to a production method for microbial flocculants, and aims to solve the problems of long production cycle of existing flocculant production methods and poor flocculation effect of flocculants. The method includes 1, picking acinetobacter and inoculating the same into a rich culture medium to obtain an active bacterium solution, and inoculating the bacterium solution into a flocculation culture medium; 2, performing fermentation cultivation to obtain a fermentation broth; 3, centrifuging the fermentation broth to collect thallus sediments, adding a bacteria lysate into the sediments, performing ultrasonic disruption, collecting a supernatant through centrifugation, adding CTAB (cetyltrimethyl ammonium bromide), collecting sediments after standing and centrifuging, adding absolute ethyl alcohol and acetone, collecting a supernatant through centrifugation, adding ethyl alcohol, collecting sediments through centrifugation, and washing the sediments which are the microbial flocculants. The production method applied to the field of waste water treatment is short in production cycle, and the flocculation effect of the microbial flocculants is good.

Description

technical field [0001] The invention relates to a production method of flocculant. Background technique [0002] Flocculation technology is currently the main method used in water supply and wastewater treatment, and its effect mainly depends on the type of flocculant. In the history of flocculation technology development, the treatment effect of inorganic salt flocculants (such as aluminum sulfate, ferric chloride, etc.) is not ideal. However, although the polymeric inorganic salt type flocculant (such as polyaluminum chloride, polyferric sulfate, etc.) has a good treatment effect, it is used in a large amount and has secondary pollution to the environment. For example, aluminum ions can poison aquatic organisms and microorganisms, pass through the food chain and drinking water, and ultimately endanger human health. However, iron-based flocculants are corrosive to metals and color the water, which limits the application of aluminum and iron salt flocculants. Organic poly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C02F1/56C12R1/01
Inventor 李琳马放李昂赵丹魏薇王爽
Owner HARBIN INST OF TECH
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