A kind of bacillus subtilis bacterial strain and application thereof

Abstract

The invention provides a Bacillus subtilis strain (Bacillus subtilis) SB13, which was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on February 28, 2014, and the preservation number is CGMCC No.8867; The compound solution containing both endoglucanase and xylanase prepared by Bacillus subtilis SB13, that is, Bacillus subtilis SB13 can ferment and grow with tofu wastewater as the only nutrient , and at the same time produce endoglucanase and xylanase enzyme liquid, thereby increasing the way for the recycling of tofu wastewater, and at the same time for the production of endoglucanase and xylanase preparations in the feed processing industry A new source of fermentation bacteria is provided.

Description

【Technical field】 [0001] The invention relates to a microorganism and its application, in particular to a bacillus subtilis strain, which can produce endoglucanase and xylanase simultaneously by utilizing tofu wastewater. 【Background technique】 [0002] In order to make full use of resources and save costs, substances with high cellulose and hemicellulose content, such as rice bran, wheat flour, cottonseed meal, straw powder, pasture, etc., are often used as raw materials to replace high-priced corn, soybeans, wheat and other substances; However, a high proportion of crude fiber diets not only cannot be directly absorbed and utilized by livestock and poultry, but also easily cause various problems such as diarrhea and slow growth of livestock and poultry. In order to improve feed quality, feed mills and farms have fully realized the importance of adding enzymes to feed, especially non-digestive enzymes such as cellulase and hemicellulase that cannot be synthesized by animals...

AI Technical Summary

Problems solved by technology

At present, most of the widely used fine strains are Trichoderma, Penicillium, Aspergillus niger and other fungi. Due to the long production cycle used by such fungi to produce cellulase, a large amount of toxic substances are also produced while producing enzymes. , the crude enzymes produced by its metabolism cannot be directly added to the feed, so technical procedures such as membrane filtration to remove toxic substances to purify cellulase are basically an indispensable link in the production of industrial cellulase preparations, not to mention A large amount of loss of enzymes occurs in the process of removing toxic substances, and the use of this production procedure and the long production cycle also greatly increase the production cost of the enterprise

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