Extraction method for producing baicalin with biological enzyme method

An extraction method and a biological enzymatic technology are applied in the field of extracting baicalin, which can solve the problems of low baicalin yield, complex process and high cost, and achieve the advantages of improving yield, strong anticancer reaction, and scavenging oxygen free radicals. Effect

Inactive Publication Date: 2015-07-22
宝鸡市虹源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The yield of baicalin produced by the above process is low, the process procedure is complicated, the efficiency is low, and the cost is high

Method used

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  • Extraction method for producing baicalin with biological enzyme method

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example 1

[0032] Example 1: Take 1 kg of black rice, crush it into fine powder, add 8 times the amount of water, adjust the pH to 5.0 with hydrochloric acid, add amylase, keep it at 70°C for 4 hours, filter, pass the filtrate through AB-8 macroporous adsorption resin, and pour the column liquid Concentrate to a density of 1.30 to obtain 821 g of black rice syrup. The resin was washed with 5 times of purified water, and then analyzed with 80% ethanol. The analyzed solution was concentrated under reduced pressure at 50°C to a density of 1.10, and spray-dried to obtain black rice anthocyanins, 11.2g, with a content of 25.8%. Add 4 times the amount of water to the filter residue, adjust the pH to 4.8 with citric acid, keep it at 50°C for 4 hours, filter, add 8 times the amount of 4% sodium hydroxide solution to the filter cake, keep it at 70°C for 2 hours, filter, and adjust the pH of the filtrate to 3.5 with hydrochloric acid , heated to 85°C, let stand, filter, filter cake, and dry at 70-...

example 2

[0033] Example 2: Take 1 kg of black rice dregs, grind it into fine powder, add 5 times the amount of water, adjust the pH to 4.5 with citric acid, add glucoamylase, keep it at 90°C for 2 hours, filter, and the filtrate passes through XDA-6 macroporous adsorption resin. The lower column liquid was concentrated to a density of 1.32 to obtain 546 g of black rice syrup. The resin was washed with 5 times of purified water, analyzed with 60% ethanol, concentrated under reduced pressure at 60°C to a density of 1.15, and freeze-dried at -40°C to obtain 13.4g of black rice anthocyanins with a content of 27.2%. Add 8 times the amount of water to the filter residue, adjust the pH to 4.8 with citric acid, keep it at 50°C for 4 hours, filter, add 6 times the amount of 4% sodium hydroxide solution to the filter cake, keep it at 70°C for 1 hour, filter, adjust the pH of the filtrate to 5 with hydrochloric acid, and heat to 98°C, let stand, filter, filter cake, add 10 times the amount of wat...

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Abstract

The invention relates to a high-yield technology for producing baicalin from a raw material, namely, baical skullcap roots, with a biological enzyme fermentation technology. According to the technical scheme, the technology comprises steps as follows: reflux extraction of the baicalin is performed on a raw material with boiling water containing a solubilizing agent firstly, a complex enzyme is added to an extract for hydrolysis, after refined filtration, a filtrate is heated to the temperature ranging from 70 DEG C to 100 DEG C, the baicalin precipitates by the aid of hydrochloric acid, is washed with water for acid removal and then is dried, the prepared baicalin content exceeds 85%, the baicalin conforms to national standard requirements, and indexes such as the solubility, the color and the like of the baicalin reach the high-quality level. With the adoption of the technology, the production process of the baicalin is simplified, the yield is increased, the production efficiency is improved, the cost is low, and the technology is suitable for large-scale production.

Description

technical field [0001] The invention relates to a method for extracting baicalin from scutellaria baicalensis. The baicalin can be used as a raw material of skin care products, medicines and cosmetics or as an additive. Background technique [0002] Scutellaria baicalensis is a Lamiaceae plant Scutellaria baicalensis Georgi, whose roots are used as medicine. There are heat-clearing and damp-drying, cooling blood and preventing miscarriage, detoxification effect. Indications for febrile disease, upper respiratory tract infection, pulmonary fever Cough, damp-heat jaundice, pneumonia, dysentery, hemoptysis, red eyes, restless fetus, high blood pressure, carbuncle and boils. Produced in Hebei, liaoning , Shaanxi, Shanxi, Shandong, Inner Mongolia, Heilongjiang and other provinces and autonomous regions. [0003] Scutellaria baicalensis root contains baicalin, baicalein, neobaicalein, that is, skullcapflavone II, wogonin, wogono-side, etc. flavonoids class of compounds. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/539A61P31/04A61P7/10A61P29/00A61P37/08A61P35/00A61K8/97A61Q19/02A61Q19/08
Inventor 魏有良李丹范建礼
Owner 宝鸡市虹源生物科技有限公司
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