Preparation method of magnetic microspheres-based levofloxacin surface imprinted material

Abstract

The invention relates to a preparation method of a magnetic microspheres-based levofloxacin surface imprinted material. The preparation process mainly comprises three steps: preparation of magnetic microspheres; coating of the magnetic microspheres with a molecularly imprinted material; and elution of template molecules. A synthetic method provided by the invention is simple. The prepared magnetic microspheres-based surface molecularly imprinted material has specific recognition function, high adsorption capacity and rapid adsorption ability of levofloxacin and has chiral resolution function of ofloxacin, has good magnetic response and high mechanical strength, can be used as an absorption filler or a coating material, also can be used in preparation of a molecular imprinting sensor and a chip, and is of great significance for researches on specific recognition, chiral resolution and highly sensitive detection of floxacin drugs.

Description

technical field

[0001] The invention belongs to the field of new material science, and in particular relates to a preparation method of a surface imprinted material based on magnetic microspheres, and the imprinted material can specifically recognize levofloxacin. Background technique

[0002] In order to overcome various shortcomings in the recognition process of biological macromolecules, based on the understanding of the specificity of the recognition process of biological macromolecules, artificially synthesized polymer materials with molecular recognition capabilities——molecularly imprinted polymers (MIPs) have been synthesized to separate, Compounds are purified. Because molecularly imprinted polymers have the three characteristics of predetermined structure and activity, specific recognition and wide applicability, and have the advantages of being inert to harsh chemical environments, good stability, and long service life, etc., they are widely used in separation and ...

Examples

Embodiment 1

[0023] Add 2.4g of ferric chloride, 3.4g of sodium acetate and 3.4g of sodium acrylate into a mixed solvent consisting of 33.75mL of ethylene glycol and 11.25mL of diethylene glycol, and ultrasonically obtain a yellow mixed solution for 1h, which is then transferred to polytetrafluoroethylene Place in a reaction kettle made of ethylene, seal it, and place it in a muffle furnace to react for 10 hours. After the reaction, cool to room temperature, wash with methanol and water for several times, and vacuum-dry at 60°C to constant weight for later use;

[0024] Add 1mmol of levofloxacin and 4mmol of methacrylic acid into 10mL of dimethyl sulfoxide, mix, shake for 2 hours, and obtain a pre-assembly solution for later use;

[0025] Add 1.0 g of the above-prepared magnetic microspheres to 4 mL of dimethyl sulfoxide for ultrasonic dispersion, add the above-prepared pre-assembly solution, 20 mmol of ethylene glycol dimethacrylate and 50 mg of azobisisobutyronitrile, and ultrasonically d...

Embodiment 2

[0028] Add 2.4g of ferric chloride, 3.4g of sodium acetate and 3.4g of sodium acrylate into a mixed solvent consisting of 33.75mL of ethylene glycol and 11.25mL of diethylene glycol, and ultrasonically obtain a yellow mixed solution for 1h, which is then transferred to polytetrafluoroethylene Place in a reaction kettle made of ethylene, seal it, and place it in a muffle furnace to react for 10 hours. After the reaction, cool to room temperature, wash with methanol and water for several times, and vacuum-dry at 60°C to constant weight for later use;

[0029] Add 1mmol of levofloxacin and 4mmol of methacrylic acid into 10mL of dimethyl sulfoxide, mix, shake for 2 hours, and obtain a pre-assembly solution for later use;

[0030] Add 1.0 g of the above-prepared magnetic microspheres to 4 mL of dimethyl sulfoxide for ultrasonic dispersion, add the above-prepared pre-assembly solution, 20 mmol of ethylene glycol dimethacrylate and 50 mg of azobisisobutyronitrile, and ultrasonically d...

Embodiment 3

[0033] Add 2.4g of ferric chloride, 3.4g of sodium acetate and 3.4g of sodium acrylate into a mixed solvent consisting of 33.75mL of ethylene glycol and 11.25mL of diethylene glycol, and ultrasonically obtain a yellow mixed solution for 1h, which is then transferred to polytetrafluoroethylene Place in a reaction kettle made of ethylene, seal it, and place it in a muffle furnace to react for 10 hours. After the reaction, cool to room temperature, wash with methanol and water for several times, and vacuum-dry at 60°C to constant weight for later use;

[0034] Add 1mmol of levofloxacin and 4mmol of acrylic acid into 10mL of dimethyl sulfoxide, mix, shake for 2 hours, and obtain a pre-assembly solution for later use;

[0035] Add 1.0 g of the above-prepared magnetic microspheres into 4 mL of dimethyl sulfoxide for ultrasonic dispersion, add the above-prepared pre-assembly solution, 20 mmol of ethylene glycol dimethacrylate and 50 mg of azobisisobutyronitrile, and ultrasonically dis...

technical field

[0001] The invention belongs to the field of new material science, and in particular relates to a preparation method of a surface imprinted material based on magnetic microspheres, and the imprinted material can specifically recognize levofloxacin. Background technique

[0002] In order to overcome various shortcomings in the recognition process of biological macromolecules, based on the understanding of the specificity of the recognition process of biological macromolecules, artificially synthesized polymer materials with molecular recognition capabilities——molecularly imprinted polymers (MIPs) have been synthesized to separate, Compounds are purified. Because molecularly imprinted polymers have the three characteristics of predetermined structure and activity, specific recognition and wide applicability, and have the advantages of being inert to harsh chemical environments, good stability, and long service life, etc., they are widely used in separation and ...

Examples

Embodiment 1

[0023] Add 2.4g of ferric chloride, 3.4g of sodium acetate and 3.4g of sodium acrylate into a mixed solvent consisting of 33.75mL of ethylene glycol and 11.25mL of diethylene glycol, and ultrasonically obtain a yellow mixed solution for 1h, which is then transferred to polytetrafluoroethylene Place in a reaction kettle made of ethylene, seal it, and place it in a muffle furnace to react for 10 hours. After the reaction, cool to room temperature, wash with methanol and water for several times, and vacuum-dry at 60°C to constant weight for later use;

[0024] Add 1mmol of levofloxacin and 4mmol of methacrylic acid into 10mL of dimethyl sulfoxide, mix, shake for 2 hours, and obtain a pre-assembly solution for later use;

[0025] Add 1.0 g of the above-prepared magnetic microspheres to 4 mL of dimethyl sulfoxide for ultrasonic dispersion, add the above-prepared pre-assembly solution, 20 mmol of ethylene glycol dimethacrylate and 50 mg of azobisisobutyronitrile, and ultrasonically d...

Embodiment 2

[0028] Add 2.4g of ferric chloride, 3.4g of sodium acetate and 3.4g of sodium acrylate into a mixed solvent consisting of 33.75mL of ethylene glycol and 11.25mL of diethylene glycol, and ultrasonically obtain a yellow mixed solution for 1h, which is then transferred to polytetrafluoroethylene Place in a reaction kettle made of ethylene, seal it, and place it in a muffle furnace to react for 10 hours. After the reaction, cool to room temperature, wash with methanol and water for several times, and vacuum-dry at 60°C to constant weight for later use;

[0029] Add 1mmol of levofloxacin and 4mmol of methacrylic acid into 10mL of dimethyl sulfoxide, mix, shake for 2 hours, and obtain a pre-assembly solution for later use;

[0030] Add 1.0 g of the above-prepared magnetic microspheres to 4 mL of dimethyl sulfoxide for ultrasonic dispersion, add the above-prepared pre-assembly solution, 20 mmol of ethylene glycol dimethacrylate and 50 mg of azobisisobutyronitrile, and ultrasonically d...

Embodiment 3

[0033] Add 2.4g of ferric chloride, 3.4g of sodium acetate and 3.4g of sodium acrylate into a mixed solvent consisting of 33.75mL of ethylene glycol and 11.25mL of diethylene glycol, and ultrasonically obtain a yellow mixed solution for 1h, which is then transferred to polytetrafluoroethylene Place in a reaction kettle made of ethylene, seal it, and place it in a muffle furnace to react for 10 hours. After the reaction, cool to room temperature, wash with methanol and water for several times, and vacuum-dry at 60°C to constant weight for later use;

[0034] Add 1mmol of levofloxacin and 4mmol of acrylic acid into 10mL of dimethyl sulfoxide, mix, shake for 2 hours, and obtain a pre-assembly solution for later use;

[0035] Add 1.0 g of the above-prepared magnetic microspheres into 4 mL of dimethyl sulfoxide for ultrasonic dispersion, add the above-prepared pre-assembly solution, 20 mmol of ethylene glycol dimethacrylate and 50 mg of azobisisobutyronitrile, and ultrasonically dis...

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