A water-soluble cu based on rhodamine b 2+ 、hg 2+ Probe and its preparation method and application
A water-soluble, probe technology, applied in the field of metal ion analysis and detection, can solve the problems of poor selectivity, pH sensitivity, single detection ion, etc., and achieve the effect of good development prospects
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Embodiment 1
[0027] Example 1: Synthetic Probe L
[0028] Step 1: Raw material 1 was synthesized according to the method of literature Chem.Asian J.2011,6,1987-1991.
[0029] Step 2: Dissolve 55 mg of raw material 1 (0.0859 mmol) in 5 ml of acetonitrile, add 24 mg of potassium carbonate and 15 mg of potassium iodide, and add 19 mg of NH(CH 2 CH 2 SCH 2 CH 2 CH 3 ) 2 (0.0859mmol), continue the reflux reaction; after the reaction, the solution is cooled to room temperature, the precipitate is filtered off, and the filtrate is concentrated under reduced pressure; the developing agent is passed through column silica gel with dichloromethane / methanol 20:1, and vacuum-dried to obtain 35 mg probe needle L. Yield: 52%
[0030] 1 H NMR (400MHz, CDCl 3 ,ppm) δ=7.94(d,1H,J=6.3Hz),7.57(t,1H,J=15.4Hz),7.49(d,2H,J=1.6Hz),7.39(m,2H,J=19.9 Hz), 7.09(t, 1H, J=6.8Hz), 6.57(d, 2H, J=8.8Hz), 6.39(s, 2H), 6.30(s, 1H), 6.28(d, 1H, J=2.4 Hz), 4.90(s, 2H), 3.71(s, 2H), 3.35(m, 8H, J=6.9Hz), 2.73(t, 4H,...
Embodiment 2
[0033] Embodiment 2: the selectivity of the probe L of the present invention to copper ions:
[0034] The synthesized probe L was prepared with acetonitrile / water (1:1, V / V) to prepare 0.4mM mother solution, and deionized water was used to prepare 5mM solutions of various metal salts. Add 3ml of ethanol:water=4:1 (pH=7.4 buffer solution, V / V) solution in the cuvette, and then add 75 μL of 0.4mM solution of the probe molecule L of the present invention configured above , which was diluted to 10 μM. After mixing evenly, measure the absorbance value of the system in the range of 450nm-650nm. The probe molecule L has a weak absorbance value at 562nm. After adding copper ions, strong absorption occurs, and the absorbance increases by about 870 times. The color of the solution changes from colorless to For fuchsia, add other metal ions
[0035] K + , Na + , Ca 2+ ,Mg 2+ ,Mn 2+ , Ni 2+ ,Zn 2+ ,Cd 2+ ,Co 2+ ,Fe 2+ ,Fe 3+ ,Cr 3+ ,Hg 2+ ,Pb 2+ , Ag + ,Al 3+ (concentrat...
Embodiment 3
[0036] Example 3: The influence of coexisting metal ions on the ultraviolet detection of copper ions by probe molecules:
[0037] In the ethanol:water=4:1 (pH=7.4 buffer solution, V / V) solution with a concentration of 10 μM probe compound, the absorbance was significantly enhanced after adding 10 equivalents of copper ions, and then added to the probe-Cu 2+ Add other metal ions such as K to the solution + , Na + , Ca 2+ ,Mg 2+ ,Mn 2+ , Ni 2+ ,Zn 2+ ,Co 2+ ,Fe 2+ ,Fe 3+ ,Cr 3+ ,Hg 2+ ,Pb 2+ , Ag + ,Al 3+ (concentration and Cu 2+ Equivalent) after measuring the absorbance of the system, the results show that the presence of other metal ions does not affect the detection of the probe molecule L of the present invention for copper ions. It reflects the strong anti-interference performance of the probe molecule. See attached figure 1 ..
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