Novel process for preparing ascorbyl glucoside

An ascorbic acid and glucoside technology, which is applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., can solve the problems of low conversion rate of glucoside ascorbic acid, long purification process time, and high reaction temperature, and achieves a clear metabolic pathway. The fermentation process is easy to control and the effect of improving the conversion rate

Active Publication Date: 2015-07-22
HUBEI ARTEC CARBOHYDRATE CHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the known synthesis method of AA-2G uses starch and L-ascorbic acid as raw materials, catalyzed by glycosyltransferase, the reaction temperature is 60-65°C, the reaction time is 30-48h, the conversion of glucoside ascorbi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Heat and dissolve 40% oligomeric maltodextrin (DE value 6), add 10% L-calcium ascorbate to the solution under reduced conditions, adjust the pH of the reaction solution to 5.5, and add 200 U / g of isomaltose based on the mass of dextrin Generate enzyme, react in an ultrasonic reactor at 55°C for 3h, ultrasonic frequency 30KHz, add 1000U / g of glucoamylase after the reaction, hydrolyze for 1h, and perform HPLC analysis on the reaction solution, it is found that 69% of L-ascorbic acid is converted into glucoside ascorbic acid. Pass the reaction solution through the ultrafiltration membrane to remove enzymes and other macromolecular substances in the solution, add activated carbon to the ultrafiltration effluent and filter it after decolorization, the filtrate contains glucose, dextrin, glucoside ascorbic acid and L-ascorbic acid, add After a certain amount of activated yeast was fermented for 24 hours, it was detected that both the glucose and dextrin were absorbed and utili...

Embodiment 2

[0020] Heat and dissolve 5% oligomeric maltodextrin (DE value 60), add 20% L-calcium ascorbate and L-ascorbic acid mixture to the solution under reduced conditions, adjust the pH of the reaction solution to 5.0, and add 100U / g according to the mass of dextrin The isomaltoglucosyl-generating enzyme was reacted in an ultrasonic reactor at 60°C for 5 hours, and the ultrasonic frequency was 50KHz. After the reaction, 1000u / g of glucoamylase was added and hydrolyzed for 1.5 hours. HPLC analysis of the reaction solution found that 68% of L-ascorbic acid Converted to the glucoside ascorbic acid. Pass the reaction solution through an ultrafiltration membrane to remove enzymes and other macromolecular substances in the solution, add activated carbon to the ultrafiltration effluent after ultrafiltration for decolorization, and then concentrate the filtrate in a vacuum at low temperature. The concentrated filtrate contains glucose, dextrin, and glucoside Ascorbic acid and L-ascorbic acid...

Embodiment 3

[0022]15% oligomeric maltodextrin (DE value 15) or pullulan is heated and dissolved, and 15% L-sodium ascorbate or L-potassium ascorbate is added to the solution under reduced conditions to adjust the pH of the reaction solution to 5.2, according to the quality of dextrin Add 200U / g of isomaltglucosyl-generating enzyme, react in a microwave reactor at 40°C for 4h, add 1000u / g of glucoamylase after the reaction, and hydrolyze for 1.5h. HPLC analysis of the reaction solution revealed that 66% of L-ascorbic acid was converted to glucoside ascorbic acid. Pass the reaction solution through an ultrafiltration membrane to remove enzymes and other macromolecular substances in the solution, add activated carbon to the ultrafiltration effluent for ultrafiltration and decolorization, and then filter. The filtrate contains glucose, dextrin, glucoside ascorbic acid and L-ascorbic acid. A certain amount of activated yeast was added to the yeast, and after 20 hours of fermentation, it was de...

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Abstract

The invention relates to a novel process for preparing ascorbyl glucoside. Starch sugar group and L-ascorbic acid or salt of L-ascorbic acid are used as raw materials to prepare ascorbyl glucoside under the condition of an additional energy field, so that the reaction time can be obviously shortened, the conversion rate of the ascorbyl glucoside is improved, unreacted sugar is removed through a microbiological fermentation method, energy conservation, emission reduction, economy and high efficiency can be achieved by using the method, then products of which the purity is greater than 95% can be extracted through ion exchange chromatography separation, and the ascorbyl glucoside crystals of which the content is greater than 99.5% can be prepared through concentration and cooling crystallization.

Description

technical field [0001] The present invention relates to a new process for preparing glucoside ascorbic acid, more specifically, it involves using starch sugar base and L-ascorbic acid or its salt as raw materials, preparing glucoside ascorbic acid under the condition of an external energy field, and removing the glucoside ascorbic acid by microbial fermentation The unreacted sugar is separated and extracted by anion and cation exchange chromatography to obtain a product with a purity >95%, and glucoside ascorbic acid crystals are prepared by concentrated crystallization. Background technique [0002] Vitamin C, scientific name L-ascorbic acid (AA) is widely used in cosmetics, food, medicine and other fields as sour agent, reducing agent, antioxidant, bleaching agent and stabilizer because of its unique chemical structure and physiological activity. . However, vitamin C also has a series of inherent disadvantages: it is extremely unstable in aqueous solution; it is easily...

Claims

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Application Information

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IPC IPC(8): C12P19/60C07H1/06C07H17/04
Inventor 李丽
Owner HUBEI ARTEC CARBOHYDRATE CHEM
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