A kind of bacillus cereus fnxj1‑2‑3 resistant to electroplating nickel bath solution for aluminum profiles and its application
A technology of Bacillus cereus and electroplating nickel, applied in the direction of bacteria, microorganism-based methods, water/sludge/sewage treatment, etc., can solve the problems of complex heavy metal components, waste of resources, pollution and hazards, and achieve good application prospects , the effect of excellent nickel enrichment ability and growth activity
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Embodiment 1
[0028] Example 1: Isolation, purification and identification of Bacillus cereus FNXJ1-2-3
[0029] 1. Separation and purification
[0030] Enrichment culture: take the activated sludge (moisture content is 16.94 ± 1.47%, pH is 5.09 ± 0.21, soil organic matter is 304.85 ± 6.95g / kg, total nitrogen 41.90±3.10g / kg, total phosphorus 24.95±2.05g / kg), packaged in aseptic bags and stored in a 4°C refrigerator. Diluted with distilled water to 1 times, 1 / 2 times, 1 / 3 times, 1 / 4 times, 1 / 5 times, 1 / 10 times and 1 / 20 times of 100mL electroplating nickel bath solution for aluminum profiles and collected activity Put about 10L of sludge together into a 250mL Erlenmeyer flask equipped with glass beads. In addition, use double distilled water to make a comparison in the same way. Carry out aerobic culture and anaerobic culture sealed in liquid paraffin in a constant temperature shaker at 33°C and 160r / min for 7-15 days to obtain enriched culture solution.
[0031]Primary screening: Take 1m...
Embodiment 2
[0041] Embodiment 2: the electroplating nickel bath solution treatment effect of aluminum profiles under aerobic and anaerobic conditions of Bacillus cereus FNXJ1-2-3
[0042] Take 1000ml of electroplating nickel bath solution for aluminum profiles, and add K to it successively 2 HPO 4 4.35g, KH 2 PO 4 1.7g, NH 4 NO 3 1.05g, MgSO 4 ·7H 2 O 0.2g, MnSO 4 0.05g, FeSO 4 ·7H 2 O 0.001g, adjust the pH to 4.2 with 1mol / L HCl to obtain a fermentation broth.
[0043] The bacillus cereus FNXJ1-2-3 that embodiment 1 obtains is diluted into the bacterium suspension of appropriate concentration with sterile water, inoculates in the fermentation broth of 100mL, makes the inoculum size of bacillus cereus FNXJ1-2-3 be 3.38×10 7 strains / ml fermentation broth, and then cultured in a 35°C water bath with a constant temperature shaker for 4 days. The culture is divided into aerobic group and anaerobic group, wherein the aerobic group is 160r / min shaking culture, the bottled volume ...
Embodiment 3
[0048] Embodiment 3 investigates the treatment effect of aluminum profile nickel electroplating bath solution with different inoculum amounts of Bacillus cereus FNXJ1-2-3
[0049] Substantially the same as in Example 2, the difference is that the inoculum size of Bacillus cereus FNXJ1-2-3 was investigated to be 1.0×10 7 strain / ml fermentation broth, 2.0×10 7 strain / ml fermentation broth, 4.0×10 7 strain / ml fermentation medium, 5.0×10 7 strains / ml fermentation broth, 8.0×10 7 The treatment of aluminum profile nickel electroplating bath solution when each strain / ml fermentation culture solution; the culture condition is 35°C, 160r / min; the bottled volume of the culture solution is 35%.
[0050] After cultivating for 4 days, the bacteria content and nickel content in the culture solution were measured, and the nickel-rich rate (%) was calculated. The results are shown in Table 3.
[0051] The test result of Bacillus cereus FNXJ1-2-3 fermented and cultivated for 4 days with di...
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