Technology for extracting collagen peptide, dermatan sulfate, hydroxyapatite and melanin from black sharkskin

A technology of dermatan sulfate and hydroxyapatite, applied in phosphorus compounds, non-metallic elements, chemical instruments and methods, etc., can solve the problem of no co-production patent report, etc., achieve high utilization rate of raw materials, reduce waste pollution, simple craftsmanship

Active Publication Date: 2015-08-26
福州宏东食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no relevant co-production patent report

Method used

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  • Technology for extracting collagen peptide, dermatan sulfate, hydroxyapatite and melanin from black sharkskin
  • Technology for extracting collagen peptide, dermatan sulfate, hydroxyapatite and melanin from black sharkskin
  • Technology for extracting collagen peptide, dermatan sulfate, hydroxyapatite and melanin from black sharkskin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Crush 100kg of black shark dry skin to 25 mesh, add 400kg of purified water, raise the temperature to 90°C, keep it for 1 hour, cool down to 53°C, adjust the pH value to 8.3, add 1kg of compound protease (0.5kg alkaline protease and 0.5kg trypsin ), stirring the enzymolysis at 53°C for 10 hours, adjusting the pH value to 4.5, raising the temperature to 90°C and heating to inactivate the enzyme for 20 minutes, cooling to 50°C, and performing centrifugation, and the separated supernatant was subjected to ultrafiltration through a 10kDa membrane. The filtered permeate was subjected to nanofiltration (cut-off of 300 Daltons) for desalination, decolorization and deodorization of large-pore adsorption resin to obtain 42.79 kg of collagen peptide. Add 4 times of 95% alcohol to the ultrafiltration concentrate, let it stand for 2 hours, obtain a precipitate, add water to dissolve the precipitate, then adjust the pH to 11, add 1% hydrogen peroxide by weight of the solution, let st...

Embodiment 2

[0040] Crush 100kg of black shark dry skin to 30 mesh, add 600kg of purified water, raise the temperature to 85°C, keep it for 2 hours, cool down to 55°C, adjust the pH value to 8.0, add 2kg of compound protease (1kg of alkaline protease and 1kg of collagenase), Stir the enzymolysis at 55°C for 11 hours, adjust the pH value to 4.0, heat up to 80°C to inactivate the enzyme for 30 minutes, cool down to 60°C, and perform centrifugation. The separated supernatant is subjected to ultrafiltration through a 15kDa membrane. For ultrafiltration The permeate was subjected to nanofiltration (with a cut-off of 300 Daltons) for desalination, decolorization and deodorization of the large-pore adsorption resin to obtain 42.65 kg of collagen peptide. Add 5 times of 92% alcohol to the ultrafiltration concentrate, let it stand for 3 hours to obtain a precipitate, add water to dissolve the precipitate, then adjust the pH to 11.5, add 1.5% hydrogen peroxide by weight of the solution, let it stand ...

Embodiment 3

[0042]Crush 100kg of black shark dry skin to 30 mesh, add 500kg of purified water, raise the temperature to 90°C, keep it for 1.5 hours, cool down to 50°C, adjust the pH value to 7.5, add 2kg of compound protease (1.0kg of papain and 1kg of collagenase), Stir the enzymatic hydrolysis at 50°C for 12 hours, adjust the pH value to 5.0, heat up to 85°C to inactivate the enzyme for 25 minutes, cool down to 55°C, and perform centrifugation. The separated supernatant is adsorbed by dermatan sulfate resin. The liquid was subjected to nanofiltration (cut-off of 300 Daltons) for desalination, macroporous adsorption resin decolorization, and deodorization to obtain 42.56 kg of collagen peptides. Add 5 times of 92% alcohol to the eluting concentrated solution, let it stand for 4 hours, obtain a precipitate, add water to dissolve the precipitate, then adjust the pH to 10.5, add 1.0% hydrogen peroxide by solution weight, let stand for 8 hours, Filtrate, adjust the pH of the filtrate to 6.3,...

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Abstract

The invention discloses a technology for extracting collagen peptide, dermatan sulfate, hydroxyapatite and melanin from black sharkskin. The technology comprises the following steps: conducting pre-treatment on the black sharkskin; carrying out enzymolysis; carrying out centrifugal separation to separate out a clear liquid and solid sediment; conducting ultrafiltration or resin adsorption on the clear liquid to obtain collagen peptide or dermatan sulfate respectively; treating the solid sediment with NaOH; conducting centrifugal separation on the treated solid sediment to obtain hydroxyapatite and melanin. The technology has the characteristics that collagen peptide, dermatan sulfate, hydroxyapatite and melanin are obtained continuously; the comprehensive utilization of the sharkskin is realized; the discharge of waste materials in the extraction and preparation processes is reduced; the production cost is reduced; the economic efficiency is improved; the technology is simple; industrialization can be realized.

Description

technical field [0001] A process for extracting collagen peptide, dermatan sulfate, hydroxyapatite and melanin from black shark skin belongs to the field of natural product extraction. Background technique [0002] Collagen peptide is easy to digest and absorb, has many physiological functions such as protecting gastric mucosa to prevent gastric ulcer, promoting calcium absorption to prevent osteoporosis, promoting skin collagen metabolism, inhibiting blood pressure rise, and reducing serum cholesterol content, etc., which leads to the demand for collagen peptide in the market. The demand is constantly increasing. [0003] Dermatan sulfate is a kind of glycosaminoglycan, which belongs to glycosaminoglycan, and its disaccharide is mainly composed of N-acetylgalactosamine and L-iduronic acid with β-1,4 or β-1,3 bonds connected. It is a sugar-containing fragment in the overall molecular structure of proteoglycans, which is covalently linked to the nucleoprotein of proteoglyca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C08B37/08C01B25/32C09B61/00
Inventor 焦广飞
Owner 福州宏东食品有限公司
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