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Probiotics double-layered microcapsule and manufacturing method thereof

A technology of microcapsules and probiotics, applied in the biological field, can solve the problems of easy water loss, bacterial cell death, etc., and achieve the effects of low cost of raw materials, good storage performance, and significant water loss resistance.

Inactive Publication Date: 2015-09-09
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention improves on the defects existing in the prior art, in view of most probiotics to O 2 And the low pH in the upper gastrointestinal tract is relatively sensitive. In order to improve the tolerance of probiotics to adverse external environments and improve the problem of bacterial death caused by traditional single-layer capsules, the present invention increases the amount of probiotics embedded in traditional single-layer capsules. The second layer of film prevents water loss and produces double-layer microcapsules of probiotics

Method used

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  • Probiotics double-layered microcapsule and manufacturing method thereof
  • Probiotics double-layered microcapsule and manufacturing method thereof
  • Probiotics double-layered microcapsule and manufacturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1) Experimental method

[0053] Determine the level range of each factor through single factor experiment, and then pass L 9 (3 4 ) Orthogonal experiments to screen the best double-layer microcapsules. After obtaining the best formula, carry out property comprehensive experiments (simulating gastric acid resistance, enteric coating, storage experiments) and conduct comparisons to remove its properties;

[0054] Preparation of microcapsules: mix bacterial powder and wall material raw materials, add deionized water, and emulsify with 200Bar high pressure for 10 minutes. The resulting emulsion was quickly dropped into CaCl 2 The solution (solidified solution) was left to solidify for 20 minutes. Implant chitosan with CaCl after collecting washed capsules 2 The solution was formed into a film for 20 minutes, collected and washed again to obtain double-layer microcapsules;

[0055] (1) Single factor experiment of microcapsule preparation:

[0056] The experiment deter...

Embodiment 2

[0088] Embodiment 2 Effect experiment of each wall material on embedding rate

[0089] 1. Set each wall material separately for single factor experiment

[0090] In this experiment, the sodium alginate in the inner capsule is set as a constant: 2% sodium alginate, CaCl 2 2%, off

[0091] Fat milk powder 3%, lactose 4%, chitosan 0.2%;

[0092]The results showed that: when the concentration of sodium alginate was too low, bacteria could not be effectively embedded, and the obtained capsules were soft, non-spherical, with tail-like structures, or even flocculent precipitates, which did not meet the experimental requirements. and when the concentration is too high, the viscosity of the bacteria emulsion is too high, homogenization is difficult, and the resulting capsule texture is too hard to be easily uncapsulated. During the orthogonal experiment in the present invention, the sodium alginate level is adjusted to: 2%, 2.5% , 3%;

[0093] Set sodium alginate as a variable: the...

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Abstract

The invention belongs to the field of biotechnology, and particularly relates to a probiotics double-layered microcapsule and a manufacturing method thereof. The probiotics double-layered microcapsule and the manufacturing method are improved according to defects in the prior art. A high-pressure homogeneous emulsification method is adopted; sodium alginate and chitosan serve as capsule wall materials and are cross-linked with calcium chloride to form a double-layer film; bifidobacterium is taken as an experimental subject; an L9(34) orthogonal experiment is designed, and the double-layered microcapsule is manufactured. The double-layered microcapsule is high in encapsulation rate, the problem that excessive water loss is likely to happen to a single-layer capsule and accordingly activated probiotics is dead can be solved, and resistance to outside bad environments of probiotics can be improved. The method is easy to operate. Cost of raw materials is low. The survival rate of probiotics bodies can be obviously increased. Cost performance is high.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a probiotic double-layer microcapsule and a preparation method thereof. Background technique [0002] At present, the health care effects of various probiotics are generally recognized. In the treatment of diarrhea and other gastrointestinal diseases, probiotic preparations are widely used and play an important role in adjuvant therapy. According to the actual application, this kind of preparation has strict requirements on the storage environment, especially the need to be sealed and refrigerated. Once the user ignores the storage conditions, it is easy to make the drug invalid. Studies have shown that probiotics are extremely sensitive to oxygen and have poor resistance to low pH in the upper gastrointestinal tract (such as gastric acid, etc.). Therefore, after long-term storage in an inappropriate way and oral administration, the number of bacteria that can co...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61K47/36A61K47/04A61K35/745A61P1/12
Inventor 莫炜周计宇张艳玲
Owner FUDAN UNIV
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