Method for improving infection rate of hosts of parasites of cordyceps sinensis

A Cordyceps sinensis host and infection rate technology, applied in botany equipment and methods, horticulture, plant cultivation, etc., can solve the problems of low larvae survival rate, low mortality rate, slow operation speed, etc., to improve infection rate and survival rate, The effect of high survival rate and easy operation

Active Publication Date: 2015-09-23
SYNGEN BIOTECH
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Problems solved by technology

For example, Chinese patent CN201010604460.7 discloses a method of infecting the larvae of the bat moth by injecting the spore liquid of Trichosporum sinensis into the larvae of the bat moth with a needle-like instrument. Due to inadvertent operation, the wound on the surface of the worm body is too large, and the larvae lose too much body fluid and die
The shortcoming of this infection method is: just reach infection peak period (infection rate 100%) after infecting 6-12 months, infect ossified time as long as 1 year, after 1 year, larva survival rate is low, and the output of relative Cordyceps sinensis fruiting body is also low
[0007] Based on the aforementioned studies, although the artificial infection of Cordyceps sinensis hosts has been achieved at present, the existing technology still has its shortcomings, which include: high host mortality after infection, low infection rate, and long infection ossification time Existing technology cannot simultaneously achieve low mortality, high infection rate and shorten infection ossification time

Method used

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  • Method for improving infection rate of hosts of parasites of cordyceps sinensis
  • Method for improving infection rate of hosts of parasites of cordyceps sinensis
  • Method for improving infection rate of hosts of parasites of cordyceps sinensis

Examples

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Effect test

Embodiment 1

[0033] The preparation method of embodiment 1 Mortierella chinensis liquid

[0034] After the sterile scalpel was sterilized under the flame of an alcohol lamp, soaked in 75% alcohol to cool down, the tissue block of the fruiting body of Cordyceps sinensis was cut with the sterile scalpel, and the tissue block was inoculated on the artificial medium. The composition of the artificial medium includes 2-10% peptone, 2-10% yeast extract, 2-10% glucose, 2-10% special sand and 1-5% agar powder, etc. Sterilization, the sterilization pressure is 1.2atm (kg / cm 2 ), the sterilization temperature is 120-130° C., and the sterilization time is about 50 minutes from the beginning to the end of the sterilization. The artificial culture medium sterilized by high temperature and high pressure can be used after being irradiated and sterilized by UV lamp for 12 hours. The tissue block of the fruit body of Cordyceps sinensis is artificially cultured in an artificial culture medium at a tempera...

Embodiment 2

[0037] Embodiment 2 The method one of China's Trichosporum solution infecting bat moth larvae

[0038] Collect 2100 bat moth larvae of 3-4 instars, divide them into 7 groups, 300 in each group, randomly take 1 group of 300 bat moth larvae, and inject the Mortierella sinensis solution prepared in Example 1 with a micro-injection needle into the larvae as a control group. The remaining 1,800 larvae were irradiated with laser light on the body surface of bat moth larvae, and were divided into 6 groups according to the irradiated body (head, tail) and laser power conditions (0.5W, 1.5W, and 2.5W), with 300 larvae in each group insect. Use a sterile cotton swab dipped in iodine to wipe the surface of the worm back and forth several times, and irradiate the surface of the worm with a laser gun head according to the above 6 groups of conditions. The irradiation time of each group is 1 second, and the same part is irradiated 3-4 times; Put the Trichomonas sinensis solution described...

Embodiment 3

[0041] Embodiment 3 Containing the rearing substrate of Mortierella sinensis liquid

[0042]The composition of the feeding substrate is designed with reference to the composition of alpine meadow soil, including humus soil, river sand and sawdust, and its optimal weight ratio is 15-25% of humus soil, 20-30% of river sand, and 50-65% of sawdust.

[0043] The preparation method of this feeding matrix is ​​as follows:

[0044] After the above-mentioned feeding matrix components are mixed, they are sterilized under high temperature and high pressure, and the pressure is about 1.2atm (kg / cm 2 ), the temperature is 120-130°C, and the sterilization time is 120-180 minutes from the beginning to the end of the sterilization. After the sterilized feed matrix is ​​tested and analyzed for sterility, the number of miscellaneous bacteria must be less than 102CFU / g.

[0045] Add the Mortierella sinensis liquid prepared in Example 1 to the above-mentioned mixed matrix, the added weight is 20...

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Abstract

The invention relates to a method for improving infection rate of hosts of parasites of cordyceps sinensis. By the method, body surfaces of larvas of the hosts of the parasites of the cordyceps sinensis are illuminated by laser light, tiny wounds are generated, hirsutella sinensis bacteria can enter body cavities of the larvas of the hosts of the parasites easily, and fruiting bodies of the cordyceps sinensis can be generated. The index components of the fruiting bodies, which are obtained by the artificial culture method, of the cordyceps sinensis are the same with those of fruiting bodies of wild cordyceps sinensis. By the method, the infection rate and the survival rate of the larvas can be improved greatly and are not limited by degree of proficiency of operators, the larvas of the hosts of the parasites of the cordyceps sinensis can be infected quickly and widely, the problem that the infection rate and the survival rate of the larvas, which are infected artificially in the prior art, of the hosts of the parasites of the cordyceps sinensis are low is solved, and the method can be used for artificial large-scale breeding and production of the cordyceps sinensis and has an important popularization role in artificial cultivation of the cordyceps sinensis.

Description

technical field [0001] The invention relates to a method for fungus infecting a host, in particular to a method for increasing the infection rate of a host of Cordyceps sinensis. Background technique [0002] Cordyceps sinensis (Berk.) Sacc. is a rare and precious Chinese herbal medicine produced in some areas of the Qinghai-Tibet Plateau above an altitude of 3,500 meters. Realize the truth, tonify deficiency, detoxify, stop bleeding, and reduce phlegm. [0003] Cordyceps sinensis belongs to insect parasitic fungi or entomogenic fungi, which is a complex formed by Hirsutella sinensis and Hepialidae larvae. The host is the larvae of the Lepidoptera bat moth, and the Trichosporum sinensis parasitizing it absorbs its nutrients until the host's tissue cells are consumed, and the mycelium and the bat moth form a hard and full endophytic sclerotia ( endosclerotium), after the formation of sclerotia, the fruiting body of Cordyceps sinensis grows under the appropriate environment....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 陳威仁葉美良魏鈺珊
Owner SYNGEN BIOTECH
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