Application of naturally-sourced and/or self-assembled biological membrane, closed structure or cell compartment with biological membrane property as medical high polymer material

A self-assembly technology, a technology of polymer materials, applied to other methods of inserting foreign genetic materials, recombinant DNA technology, medical preparations of non-active ingredients, etc., to achieve high safety, rich technical support, and high utilization.

Inactive Publication Date: 2015-09-23
HANGZHOU UMOTOR BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In order to overcome the deficiencies of artificial membranes that still have application defects at present, the purpose of the present invention is to provide bio

Method used

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  • Application of naturally-sourced and/or self-assembled biological membrane, closed structure or cell compartment with biological membrane property as medical high polymer material
  • Application of naturally-sourced and/or self-assembled biological membrane, closed structure or cell compartment with biological membrane property as medical high polymer material
  • Application of naturally-sourced and/or self-assembled biological membrane, closed structure or cell compartment with biological membrane property as medical high polymer material

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0042] Example 1 Extraction and purification of biofilm

[0043] Use density gradient centrifugation to extract and purify the desired biofilm. The specific process is as follows:

[0044] (1) The New Zealand white rabbits fasted for 18-24 hours were sacrificed to take the liver, after removing the large blood vessels, the liver was cut into 2mm 3 The left and right small pieces, and rinse with normal saline until the tissue pieces turn white;

[0045] (2) Preparation of homogenate: 1mmol / L CaCl 2 , 50mmol / L HEPES (pH 7.4), 1mmol / L PMSF, 2μg / mL aprotinin, 2μg / mL Antipain;

[0046] (3) Add the liver tissue and homogenate to 8 times the volume of the homogenate in a mass:volume ratio, and homogenize it with an electric homogenizer at 15,000 rpm in an ice bath environment until the tissue is liquefied;

[0047] (4) Filter the homogenate through four layers of gauze, centrifuge the filtrate at 1,000×g, 10min, 4℃, and collect the precipitate;

[0048] (5) Sucrose solution A for precipitatio...

Example Embodiment

[0050] Example 2 Extraction and purification of biofilm

[0051] A two-phase partition method is used to extract and purify the desired biofilm. The specific process is as follows:

[0052] (1) Screen full and consistent normal corn seeds, soak and disinfect them in 1% NaClO for 10 minutes, rinse them, and germinate for 72 hours at a constant temperature of 25°C in the dark;

[0053] (2) Take corn epicotyls and add 2 times the volume of extraction buffer (5mmol / L EDTA, 25mmol / L Tris, 0.25mmol / L sucrose, 1mmol / L MgSO) in a mass:volume ratio 4 , 0.2% (W / V) BSA, 0.5% (W / V) PVP-10, 10% (W / V) glycerol, 15mmol / L β-mercaptoethanol, 1mmol / L PMSF, 1mmol / L DTT), ice Bath grind to liquefaction;

[0054] (3) Filter the grinding liquid through four layers of gauze, centrifuge the filtrate at 12,000×g, 15min, 4℃, and take the supernatant;

[0055] (4) Centrifuge the supernatant at 80,000×g, 30min, 4℃, and collect the precipitate;

[0056] (5) Suspension for precipitation (25mmol / L Tris, 0.25mmol / L ...

Example Embodiment

[0058] Example 3 Extraction and purification of biofilm

[0059] Use differential centrifugation to extract and purify the required biofilm. The specific process is as follows:

[0060] (1) Antarctic ice algae ( Chlamydomonas subcaudata ) Is isolated and purified from Antarctic sea ice;

[0061] (2) Inoculate Antarctic ice algae into the culture medium at a ratio of 1:100 (each 10L medium contains 21.2g NaCl, 3.6g NaSO 4 , 0.6g KCl, 0.3g NaHCO 3 , 0.1g KBr, 0.1g H 3 BO 3 , 0.1g NaF, 9.6g MgCl 2 ·6H 2 O, 1.0g CaCl 2 , 0.1g SrCl 2 ·6H 2 O), placed in a controlled light incubator for cultivation. Cultivate for 14 days under conditions of -4℃, light intensity of 1300~1900lx, light cycle of 12 bright / 12 dark, shaking 4~5 times a day;

[0062] (3) Centrifuge the ice algae culture solution at 4,000 rpm, 20 min, 4°C, collect the ice algae precipitate, and quickly rinse twice with pre-cooled distilled water to remove the extracellular viscous material, surface salt and impurities in the cul...

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Abstract

The invention belongs to the field of biological medicine and high polymer materials, and particularly relates to an application of a naturally-sourced and/or self-assembled biological membrane and a closed structure and a cell compartment with the biological membrane property as a medical high polymer material. The biological membrane, the closed structure or the cell compartment with the biological membrane property are sourced from animals, plants or microorganisms. The prepared biological membrane is complete or fragmental and is of a lipoid two-molecule-layer structure on the morphology, main components of the prepared biological membrane are lipoid and proteins, and the prepared biological membrane is additionally provided with little sugar combined to the lipoid or proteins by virtue of a covalent bond. The biological membrane, the closed structure or the cell compartment with the biological membrane property is used as a substrate, and cells or tissues are cultivated on the substrate; being used as a strut of an artificial organ or tissue regenerative restoration product, the biological membrane and the closed structure or the cell compartment with the biological membrane property is applied to the organ transplantation, so that not only is safety high, the rejection phenomenon after the strut is planted into the human body can be avoided, but also the performance is similar to that of the original tissues, the availability is high, and technical support is supplied for the research and application of the high polymer material.

Description

technical field [0001] The invention belongs to the field of biomedicine and macromolecular materials, and specifically relates to the application of biofilms obtained from natural sources and / or self-assembly, closed structures with biofilm properties, and cell compartments as medical macromolecular materials. Background technique [0002] Biomembranes or biological membranes are the general term for all membrane structures at the junction of cells, organelles and their environment. They play a role in dividing and separating cells and organelles, and are also important parts related to intracellular communication. All organisms have biofilms except some viruses. In addition to the plasma membrane (also known as the cell membrane), eukaryotic cells also have a membrane system that separates various organelles, including nuclear membranes, mitochondrial membranes, endoplasmic reticulum membranes, lysosome membranes, Golgi membranes, chloroplast membranes, vacuoles, and percu...

Claims

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Application Information

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IPC IPC(8): A61K47/46A61K8/96A61K39/39A61P31/12A61P35/00A61Q19/02A61Q19/08A61Q19/00A61L27/36C12N15/87
Inventor 高颖姚震祥王旻子魏建良
Owner HANGZHOU UMOTOR BIOTECH CO LTD
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