Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Pleurotus eryngii Quel. liquid strain capable of preventing mixed bacterium infection during cultivation and fermentation method of pleurotus eryngii Quel liquid strain

A liquid strain and fermentation method technology, applied in microorganism-based methods, cultivation, plant cultivation and other directions, can solve the problems of shortening the bacterial age, proliferation generation gap, small diameter of the mycelium ball, etc., to shorten the bacterial age and The effect of proliferation algebra gap, uniform distribution and small diameter of mycelial ball

Inactive Publication Date: 2015-09-30
TIANJIN ZHONGTIAN JINGKE SCI & TECH +1
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem solved by the present invention is to overcome the defects of the existing Pleurotus eryngii liquid strains prepared by aerated fermentation, and the scientific compounding in the medium of each stage of the strain cultivation process can improve the cold resistance, heat stress and immunity of the strains. The active Chinese herbal medicine extract and the adaptive raw material sawdust powder are added inoculated in stages and fermented at variable temperatures to shorten the age and proliferation algebra gap of the strains, and the coated chitosan is added in a timely manner to adjust the viscosity of the fermentation medium. Cultivate a Pleurotus eryngii liquid strain with strong fermented hyphae, high activity, small mycelium ball diameter, high density, uniform distribution, and short fermentation period

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1 raw material preparation

[0052] 1. Preparation of Chinese herbal medicine extract:

[0053] The preparation method of the Chinese herbal medicine extract comprises the steps of: weighing 30 parts of astragalus, 30 parts of dried ginger, 30 parts of tangerine peel, 25 parts of bezoar, 25 parts of paeonol, 20 parts of licorice, and 18 parts of anemarrhena in parts by weight. 18 parts of honeysuckle, 17 parts of Houttuynia cordata, 15 parts of red peony, 15 parts of scutellaria baicalensis, 10 parts of Chuanxiong, and 8 parts of angelica; put them in an ultrasonic cleaner filled with 0.2% sodium bicarbonate solution for 12 minutes at 200W and 30KHz, Drain, crush the above-mentioned Chinese herbal medicine to a particle size of less than 2 mm, put it in a container and mix evenly and add 5 times the weight of water to obtain a mixed material, control the temperature at 80°C for 3 hours, then lower the temperature to 45°C, and adjust the pH value with lactic a...

Embodiment 2

[0061] A kind of fermentation method of the Pleurotus eryngii liquid bacterial strain that prevents miscellaneous bacteria infection during cultivation, comprises the steps:

[0062] 1) Take the preserved Pleurotus eryngii slant strain 0.2cm 2 Inoculate 2 pieces of the strain block of the above-mentioned bacteria on the plate medium for activation, and culture at a constant temperature of 25°C for 10 days, and activate twice in this way to obtain plate seeds;

[0063] 2) Use a puncher with a diameter of 0.5 cm to intercept the flat seed 0.2 cm 2 3 pieces of the strain block were inserted into a 250mL Erlenmeyer flask, and the amount of liquid seed culture medium was 100mL. They were cultured at a constant temperature of 25°C for 2 days, then placed in a constant temperature shaker, and shaken at 25°C and 100r / min for 3 days. liquid seeds;

[0064] 3) The liquid seeds were inoculated in a 500 mL shake flask with an inoculum amount of 10%, and the liquid volume of the shake fl...

Embodiment 3

[0075] A kind of fermentation method of the Pleurotus eryngii liquid bacterial strain that prevents miscellaneous bacteria infection during cultivation, comprises the steps:

[0076] Step 1) to 4) with embodiment 2;

[0077] 5) First inoculate the seeds of the seed tank with a 5% inoculation amount in a 30L fermenter, and ferment at a constant temperature of 30°C with an air flow of 3L / min for 12 hours, and then cool down to 18°C ​​at a rate of 0.6°C / min. ℃, add 8g coated chitosan and 900mL seed tank seeds into the fermenter, ferment at constant temperature for 10h under the condition of ventilation rate 6L / min, and finally raise the temperature to 24℃ at the rate of 0.3℃ / min. Fermenting at constant temperature for 45 hours under certain conditions can prevent the liquid strain of Pleurotus eryngii from being infected by miscellaneous bacteria during cultivation;

[0078] Described plate medium composition is the same as embodiment 2;

[0079] The liquid seed medium consists...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a pleurotus eryngii Quel. liquid strain capable of preventing mixed bacterium infection during cultivation and a fermentation method of the pleurotus eryngii Quel liquid strain, and belongs to the technical field of pleurotus eryngii Quel. cultivation. According to the invention, Chinese herbal medicine extracts capable of improving cold resistance, thermal stress, immunity and resistance to mixed bacterial of the strain are scientifically compounded in the culture media of liquid seeds, shake flask seeds, seeding tank seeds and fermentation tank liquid strain at all stages, meanwhile, sawdust powder with raw material adaptability and a defoaming agent function is scientifically compounded, and the mode of supplemental inoculation and segmented temperature-variable fermentation is adopted, so that the age of the strain is shortened and the proliferation generation difference is reduced; fed batch of chitosan which has a special function after being wrapped is carried out at the right moment, so that the pleurotus eryngii Quel. liquid strain which is thick and solid in fermentative hyphae, high in activity, small in mycelium pellet diameter, high in density, and uniform in distribution is cultured, the density of the mycelium pellet is that 1 L of strain contains 4.5-5.5*10<5> mycelium pellets, the diameter of the mycelium pellet is 0.5-0.7 mm, the dry weight of each mycelium pellet is 90-100 g / l, the germination time is 4-5 h and exopolysaccharide is 3.0-3.5 g / L.

Description

technical field [0001] The invention relates to the technical field of pleurotus eryngii cultivation, in particular to a liquid strain of pleurotus eryngii that prevents infection by miscellaneous bacteria during cultivation and a fermentation method thereof. Background technique [0002] Pleurotus eryngii (PleurotuseryngiiQuel.), also known as Pleurotus eryngii, has the advantages of thick flesh and crisp texture, especially its dense, firm, milky white and refreshing stipe. It is called "king of oyster mushrooms" and "scallop mushrooms". , with almond fragrance and abalone taste, suitable for fresh-keeping and processing, and is deeply loved by people. It is rich in nutrients, rich in protein, carbohydrates, vitamins and minerals such as calcium, magnesium, copper, zinc, etc., which can improve human immune function. It has the effects of anti-cancer, lowering blood fat, moisturizing the stomach and beautifying the human body, and has extremely high cultivation value and c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G1/04C12R1/645
CPCC12N1/14A01G18/00
Inventor 李建树
Owner TIANJIN ZHONGTIAN JINGKE SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com