Disinfectant for water in grass carp and prawn mixed aquaculture ponds and method for applying disinfectant

A disinfectant and pond technology, applied in the direction of disinfectant, botanical equipment and method, application, etc., can solve the problems of limited disinfectant, single preparation, corrosion of devices, etc., achieve enhanced disinfection effect, diverse action modes, and simple preparation Effect

Inactive Publication Date: 2015-10-07
盐城恒兴饲料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] From the current point of view, there are still many shortcomings in existing disinfectants, such as chlorine-containing disinfectants are easily affected by changes in water organic matter and pH, and have a corrosive effect on instruments and are also highly toxic to fish; quaternary ammonium salts The bactericidal effect of iodine preparations is easily affected by water hardness; the effect of iodine preparations is easily affected by light, temperature and other conditions, and is prone to volatilization and failure (Huang Xiaoli and Deng Yongqiang, 2004); at the same time, most of the disinfectants currently used in

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Prepare the water body disinfectant for aquaculture ponds according to the weight percentages of the following components:

[0038] Potassium Monopersulfate, 70%; Malic Acid, 5%; Sodium Chloride, 6%; Coptis Rhizoma Extract, 19%;

[0039] It is obtained by mixing the above-mentioned components uniformly according to the weight percentage;

[0040] The above-mentioned water body disinfectant was tested for the killing effect of Aeromonas:

[0041] The disinfectant was prepared into a disinfectant solution with a concentration of 0.5ppm; the medium was beef extract liquid and solid medium, pH 7.0±0.2; the reagents used were all analytically pure, and the tested strain was the standard strain of Aeromonas hydrophila For the preparation of ATCC7966 (purchased from China Agricultural Microorganism Collection Center) bacterial suspension, each strain of purely cultured Aeromonas was cultured in haploid broth, and cultured overnight on a bacterial enrichment shaker, and the 24...

Embodiment 2

[0048] Prepare the water body disinfectant for aquaculture ponds according to the weight percentages of the following components:

[0049] Potassium Monopersulfate, 50%; Malic Acid, 15%; Sodium Chloride, 10%; Coptis Rhizoma Extract, 25%.

[0050] Prepared by uniformly mixing the above compositions according to weight percentage;

[0051] Using the water body disinfectant prepared in Example 2, a water body disinfection experiment was carried out in a grass carp and prawn polyculture pond in Dafeng Fangqiang Farm in May 2014: 15 days before the prawn seedlings were stocked in a suitable place, a disinfectant solution was prepared according to a concentration of 1%. The pond was fully disinfected, and then put in prawn fry and grass carp fry and cultured according to normal steps; every 15 days after the fry, a disinfectant was prepared according to a concentration of 0.5ppm to carry out routine disinfection of the water body; the experiment was carried out for 123 days in total...

Embodiment 3

[0057] Prepare a kind of water body disinfectant for aquaculture ponds according to the weight percentages of the following components:

[0058] Potassium Monopersulfate, 57%; Malic Acid, 15%; Sodium Chloride, 2%; Coptis Rhizoma Extract, 26%;

[0059] It is obtained by mixing the above-mentioned components uniformly according to the weight percentage;

[0060] Use the water disinfectant prepared in Example 3 to conduct an experiment on the killing effect of grass carp reovirus (GCRV): Grass carp reovirus is cultured with grass carp kidney (CIK) cells, and the maintenance solution is serum-free DMEM Culture medium (containing TPCK-treated trypsin 2g / ml, 2% fetal bovine serum), calculate the median infection dose (TCID50) of the virus to the cells according to Reed-Meuench, use the maintenance solution to serially dilute the disinfectant to 12 dilutions, add to CIK cells were cultured in a 96-well culture plate in a 26° incubator, and the cell lesions were observed every day; ...

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PUM

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Abstract

The invention discloses a disinfectant for water in grass carp and prawn mixed aquaculture ponds. The disinfectant comprises, by weight, 46-78% of potassium monopersulfate, 3-15% of malic acid, 2-10% of sodium chloride and 19-35% of coptis chinensis extracts. The potassium monopersulfate, the malic acid, the sodium chloride and the coptis chinensis extracts are uniformly mixed with one another to obtain the disinfectant. The invention further discloses a method for applying the disinfectant for the water in the grass carp and prawn mixed aquaculture ponds. The disinfectant for the water in the grass carp and prawn mixed aquaculture ponds and the method for applying the disinfectant have the advantages that main pathogenic microorganisms in the aquaculture ponds can be effectively killed, adverse effects on grass carps and prawns can be prevented, and the disinfectant is free of residues and pollution.

Description

technical field [0001] The invention relates to the technical field of water body disinfection and purification in aquaculture, in particular to a water body disinfectant for grass carp and prawn polyculture ponds and a use method thereof. Background technique [0002] China is a big aquaculture country. According to statistics, my country's aquaculture volume accounts for more than two-thirds of the world's total aquaculture. However, the problem of aquatic diseases has always been a serious problem hindering the healthy development of aquaculture industry; The incidence of aquaculture diseases is over 50%, and the loss rate is as high as 20%, causing direct economic losses of more than 10 billion yuan; Huge economic losses, among which the losses caused by viral diseases are also increasing; in the process of aquatic disease prevention and control, water disinfection is used to kill pathogenic microorganisms (viruses, bacteria, etc.) in water bodies or on the surface of farm...

Claims

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Application Information

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IPC IPC(8): A01N65/32A01N59/02A01N43/90A01P1/00A01P3/00C02F1/50
Inventor 刘兴旺许丹祝焱彬梁超王爱民任鸣春
Owner 盐城恒兴饲料有限公司
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