Disinfectant for water in grass carp and prawn mixed aquaculture ponds and method for applying disinfectant
A disinfectant and pond technology, applied in the direction of disinfectant, botanical equipment and method, application, etc., can solve the problems of limited disinfectant, single preparation, corrosion of devices, etc., achieve enhanced disinfection effect, diverse action modes, and simple preparation Effect
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Embodiment 1
[0037] Prepare the water body disinfectant for aquaculture ponds according to the weight percentages of the following components:
[0038] Potassium Monopersulfate, 70%; Malic Acid, 5%; Sodium Chloride, 6%; Coptis Rhizoma Extract, 19%;
[0039] It is obtained by mixing the above-mentioned components uniformly according to the weight percentage;
[0040] The above-mentioned water body disinfectant was tested for the killing effect of Aeromonas:
[0041] The disinfectant was prepared into a disinfectant solution with a concentration of 0.5ppm; the medium was beef extract liquid and solid medium, pH 7.0±0.2; the reagents used were all analytically pure, and the tested strain was the standard strain of Aeromonas hydrophila For the preparation of ATCC7966 (purchased from China Agricultural Microorganism Collection Center) bacterial suspension, each strain of purely cultured Aeromonas was cultured in haploid broth, and cultured overnight on a bacterial enrichment shaker, and the 24...
Embodiment 2
[0048] Prepare the water body disinfectant for aquaculture ponds according to the weight percentages of the following components:
[0049] Potassium Monopersulfate, 50%; Malic Acid, 15%; Sodium Chloride, 10%; Coptis Rhizoma Extract, 25%.
[0050] Prepared by uniformly mixing the above compositions according to weight percentage;
[0051] Using the water body disinfectant prepared in Example 2, a water body disinfection experiment was carried out in a grass carp and prawn polyculture pond in Dafeng Fangqiang Farm in May 2014: 15 days before the prawn seedlings were stocked in a suitable place, a disinfectant solution was prepared according to a concentration of 1%. The pond was fully disinfected, and then put in prawn fry and grass carp fry and cultured according to normal steps; every 15 days after the fry, a disinfectant was prepared according to a concentration of 0.5ppm to carry out routine disinfection of the water body; the experiment was carried out for 123 days in total...
Embodiment 3
[0057] Prepare a kind of water body disinfectant for aquaculture ponds according to the weight percentages of the following components:
[0058] Potassium Monopersulfate, 57%; Malic Acid, 15%; Sodium Chloride, 2%; Coptis Rhizoma Extract, 26%;
[0059] It is obtained by mixing the above-mentioned components uniformly according to the weight percentage;
[0060] Use the water disinfectant prepared in Example 3 to conduct an experiment on the killing effect of grass carp reovirus (GCRV): Grass carp reovirus is cultured with grass carp kidney (CIK) cells, and the maintenance solution is serum-free DMEM Culture medium (containing TPCK-treated trypsin 2g / ml, 2% fetal bovine serum), calculate the median infection dose (TCID50) of the virus to the cells according to Reed-Meuench, use the maintenance solution to serially dilute the disinfectant to 12 dilutions, add to CIK cells were cultured in a 96-well culture plate in a 26° incubator, and the cell lesions were observed every day; ...
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