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A kind of degrading bacterial strain of herbicide 2,4-d and inoculum and application thereof

A technology of herbicide and degrading bacteria, which is applied in the field of microorganisms, can solve the problems of phytotoxicity of next-crop crops, impact on food safety, strong adsorption, etc. The effect of strong resistance

Active Publication Date: 2018-04-06
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

2,4-D degrades slowly and has strong adsorption, so it is easy to remain
In addition, the phytotoxicity of next crops caused by 2,4-D residues also causes serious economic losses and affects food security

Method used

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  • A kind of degrading bacterial strain of herbicide 2,4-d and inoculum and application thereof
  • A kind of degrading bacterial strain of herbicide 2,4-d and inoculum and application thereof
  • A kind of degrading bacterial strain of herbicide 2,4-d and inoculum and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] The invention provides a bacterial strain capable of efficiently degrading (mineralizing) 2,4-D and the bacterial agent produced therefrom. The bacterial strain used is Gram-negative bacteria LZ35, which is isolated from the soil of the Lvlilai Pesticide Factory in Kunshan, Jiangsu. . The specific isolation and screening method of the strain is: take 5.0g of soil sample and add it to 100ml of inorganic salt liquid medium with 0.1% yeast powder, add 2,4-D to a final concentration of 50ppm, and carry out enrichment culture at 30°C. The rotating speed of the shaker was 150 rpm, and 10% inoculum was inoculated into fresh inorganic salt liquid medium every 7 days, and subcultured continuously for 3 times. Then inoculate 1% of the inoculum into fresh 20ml inorganic salt liquid medium with a final concentration of 2,4-D of 50ppm, and culture for 5 days. The content of 2,4-D was detected simultaneously by ultraviolet scanner and liquid chromatography, and it was found that 2,4...

Embodiment 2

[0040] Activate the original seed of the 2,4-D herbicide-degrading strain LZ35 of the present invention on a petri dish, measure its degradation performance, and inoculate it on the inclined surface of a test tube for later use. The test tube seed is inoculated in a 1000ml shake flask containing 200ml of broth medium (3 grams of beef extract, 10 grams of peptone, 5 grams of NaCl, 1000 ml of water, pH 7.0-7.2), constant temperature shaking culture to logarithmic phase, ready to inoculate the seed tank . 500 liters of seed tanks, 400 liters of feeding capacity, the medium formula is: glucose 0.8%, (NH 4 ) 2 SO 4 1%, K 2 HPO 4 0.2%, MgSO 4 0.05%, NaCl 0.01%, CaCO 3 0.3%, yeast extract 0.02%, pH 7.2-7.5. After the feeding is completed, 121°C high-pressure damp heat sterilization, after cooling to 33°C, inoculate the above-mentioned cultivated shake flask strains into a 500-liter seed tank according to the inoculum amount of 10%, and cultivate to the logarithmic growth p...

Embodiment 3

[0042] Activate the original seed of the 2,4-D herbicide-degrading strain LZ35 of the present invention on a petri dish, measure its degradation performance, and inoculate it on the inclined surface of a test tube for later use. The test tube seed was inoculated in a 1000ml shake flask containing 500ml broth medium (3 grams of beef extract, 10 grams of peptone, 5 grams of NaCl, 1000 milliliters of water, pH 7.0-7.2), and cultured with constant temperature shaking until the logarithmic phase. The cells were collected by centrifugation, and the cells were resuspended in sterile water. Then take fresh soil for testing, add 2,4-D aqueous solution, mix thoroughly, so that the concentration of 2,4-D in the soil is 50mg·kg -1 and 100mg·kg -1 dry soil. At the same time, the treatment group was inoculated with fresh LZ35 strain suspension in the soil, and the blank control group was added with sterile water and stirred evenly. The mixed soil is divided into disposable plastic cups, ...

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Abstract

The invention discloses a herbicide 2,4‐D degrading strain and the bacterial agent produced by it and its application. It was preserved in the China Type Culture Collection Center on April 15, 2015, and the strain preservation number is CCTCC NO: M 2015235 . The strain was Gram-negative strain LZ35, which was identified as Achromobacter sp. The 2,4-D degrading strain LZ35 described in the present invention can be applied to the degradation of herbicide 2,4-D. A degrading bacterial agent produced by the 2,4-D degrading bacterial strain LZ35 of the present invention can degrade 99% of 100mg / l of 2,4-D in the liquid medium within 2 days, and direct application can significantly reduce the It solves the problem of excessive herbicide residues in agricultural production, and produces green agricultural products that are non-toxic and pollution-free.

Description

technical field [0001] The invention belongs to the field of microbes, and provides a herbicide 2,4-D degrading bacterial strain, a bacterial agent produced therefrom and an application thereof. technical background [0002] Organochlorine pesticides are one of the most important and effective pesticides used before the 1980s. Because of their low price, high efficiency and broad spectrum, they have been widely used in the world. Among them, phenoxyacetic acid herbicides are produced and used on a large scale because of their low cost and high speed. 2,4-D (2,4-dichlorophenoxyacetic acid; 2,4-Dichlorophenyloxy acetic acid) is a representative substance for the synthesis of auxin, and it is also a commonly used herbicide, which plays an important role in agriculture and forestry. It plays a significant role, especially widely used in the preservation of fruits (such as citrus). 2,4-D can be used as a plant growth regulator at a low concentration below 30ppm, and as a herbic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/025A62D101/04
CPCA62D3/02A62D2101/04A62D2101/28B09C1/10C12N1/205C12R2001/025
Inventor 蒋建东张龙杜方岭周庆新王军华陈凯李顺鹏
Owner NANJING AGRICULTURAL UNIVERSITY
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