Method for manufacturing quinclorac solid degrading inoculant
A technology of quinclorac and its production method, which is applied in the field of microorganisms, can solve the problems of tobacco plant growth deformity, plant dwarfing, leaf width suppression, etc., and achieve the effects of low production cost, promoting crop growth, and preventing the invasion of pathogens
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[0016] A kind of preparation method of quinclorac solid degrading bacterial agent, comprises the steps:
[0017] (1) Preparation of solid medium: mix wheat bran, corn stalk powder (20-30mm), and activated carbon at a mass percentage of 85%:10%:5%, then add distilled water twice the volume of the total raw materials, 121 After sterilizing at ℃ for 20 minutes, a solid medium was obtained;
[0018] (2) Pretreatment of solid medium: adjust the pH to 7.0 with NaOH aqueous solution, and sterilize at 121°C for 20 minutes;
[0019] (3) Preparation of liquid seeds: Inoculate the Bordetella strain HN36 preserved on a slant into a Erlenmeyer flask containing LB liquid medium, and culture at 35°C and 150 rpm for 36 hours with shaking (the OD value detected by a spectrophotometer is at 1.0 or so), to get liquid seeds;
[0020] (4) Based on the solid medium, inoculate the liquid seeds at 10v% (volume percentage) into the solid medium pretreated in step (2), mix well, and culture in an ele...
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