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Method for manufacturing quinclorac solid degrading inoculant

A technology of quinclorac and its production method, which is applied in the field of microorganisms, can solve the problems of tobacco plant growth deformity, plant dwarfing, leaf width suppression, etc., and achieve the effects of low production cost, promoting crop growth, and preventing the invasion of pathogens

Inactive Publication Date: 2015-10-21
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of quinclorac in paddy fields will also cause certain harm to the subsequent tobacco fields, causing abnormal growth of tobacco plants, shortening of plants, curling and shriveling of new leaves on the back, and severe suppression of leaf width

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A kind of preparation method of quinclorac solid degrading bacterial agent, comprises the steps:

[0017] (1) Preparation of solid medium: mix wheat bran, corn stalk powder (20-30mm), and activated carbon at a mass percentage of 85%:10%:5%, then add distilled water twice the volume of the total raw materials, 121 After sterilizing at ℃ for 20 minutes, a solid medium was obtained;

[0018] (2) Pretreatment of solid medium: adjust the pH to 7.0 with NaOH aqueous solution, and sterilize at 121°C for 20 minutes;

[0019] (3) Preparation of liquid seeds: Inoculate the Bordetella strain HN36 preserved on a slant into a Erlenmeyer flask containing LB liquid medium, and culture at 35°C and 150 rpm for 36 hours with shaking (the OD value detected by a spectrophotometer is at 1.0 or so), to get liquid seeds;

[0020] (4) Based on the solid medium, inoculate the liquid seeds at 10v% (volume percentage) into the solid medium pretreated in step (2), mix well, and culture in an ele...

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PUM

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Abstract

The invention provides a method for manufacturing a quinclorac solid degrading inoculant, and belongs to the technical field of microorganisms. The method includes the steps that 1, preparation, pH adjustment and sterilization of a medium are conducted; 2, bordetella preserved on a bevel is inoculated, and shake cultivation is carried out to obtain liquid seeds; 3, the liquid seeds are inoculated in a culture medium containing wheat bran, corn straw powder and activated carbon, and are cultured in a constant-temperature culture box in a sealed mode to obtain a target product. The organophosphorus pesticide degrading inoculant produced through the technology has the advantages of being low in product cost and environmentally friendly, and the inoculant can efficiently degrade organophosphorus pesticide residues on the surfaces of plants and in soil. Besides, physical and chemical properties and biological characters of the soil can be improved, soil fertility is improved, immunity of crops can be enhanced, intrusion of bacteria is prevented, and the inoculant particularly has a good prevention function on soil-borne diseases and the continuous cropping barrier. Thus, crop growth is promoted, the agricultural yield is increased, quality of agricultural products is improved, and production cost is reduced.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a preparation method of a quinclorac solid-state degrading bacterial agent. Background technique [0002] Quinclorac is a hormone-type quinolinic acid herbicide with strong selectivity. It is a new type of hormone-type quinoline carboxylic acid herbicide developed by BASF in the early 1980s, mainly used for preventing barnyardgrass in paddy fields. The application of quinclorac in paddy fields also caused certain harm to the subsequent tobacco fields, causing deformed growth of tobacco plants, shortening of plants, curling of the back of new leaves, shrinkage, and severe suppression of leaf width. Pesticides, as synthetic xenobiotic substances, are usually not easily degraded by microorganisms and have a very long half-life in nature, so they are called recalcitrant compounds. But after long-term natural selection, there are already many microorganisms that can degrad...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P21/00C09K17/00C12R1/01C09K101/00
Inventor 徐淑霞张世敏梁振普高玉千吴坤赵培贺亚斐
Owner HENAN AGRICULTURAL UNIVERSITY
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