Perfusion preservation fluid for isolated liver

Abstract

The invention relates to the technical field of organ transplantation, and particularly discloses perfusion preservation fluid for an isolated liver. The perfusion preservation fluid for the isolated liver comprises donor whole blood, albumin, antibiotics, insulin, heparin, compound amino acid, glucose, dexamethasone, sodium chloride, sodium bicarbonate, normal saline, ATP-MgCl2 and S-adenosyl methionione which are mixed according to proportion. The isolated liver is preserved in the perfusion preservation fluid for 50 hours, the damage to liver cells is less, no complication occurs, the requirements of organ transplantation can be met, the survival time of a graft can be prolonged, and the survival rate of the graft in a host is improved; the perfusion preservation fluid is low in production cost and has the practical application value.

Description

technical field [0001] The invention relates to the technical field of organ transplantation, in particular to an isolated organ perfusion preservation solution. Background technique [0002] Organ transplantation is the ultimate treatment for some diseases. Through organ transplantation, the life of some patients can be extended. A very critical step in organ transplantation is the preservation of isolated organs, which largely determines the success or failure of organ transplantation. Generally, organs cut from other donors cannot be transplanted into patients immediately, and there must be a storage facility. However, at room temperature, cells in isolated organs still undergo hypoxic metabolism, causing intracellular acidosis, mitochondrial energy production disorders, intracellular calcium overload, cell swelling, and a large number of free radicals, which eventually lead to abnormalities in isolated organs. Reversed lesions or death, therefore, require proper perfusi...

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Problems solved by technology

[0003] The clinically recognized preservation solution for isolated organs is UW solution. Its main disadvantage is that its antioxidant effect is weak. Because UW solution contains hydroxyethyl starch, the viscosity is too high and it has no effect on the preservation of isolated organs and lungs. Not good; the Chinese patent application No. 200610124269.3 discloses a new type of multi-organ storage protection solution, which uses an antioxidant system composed of melatonin, vitamin C and water-soluble vitamin E, and aims to reduce organ storage. Oxidative damage caused by free radicals; Chinese patent application number 200810036928.X discloses an isolated liver perfusion preservation solution, which is more effectively applied to the storage of isolated livers at 0-4°C, and can prevent ischemia caused by low temperature Injury, cell edema and secondary injury

[0004] In addition, in liver transplantation, there are still many transplant opportunities lost due to the limited storage time. Even if the transplant operation is successful, the liver tissue damage caused by the preservation process leads to liver quality decline and immune rejection, resulting in transplant failure. In order to improve the efficiency of liver transplant surgery success rate, it is necessary to develop a perfusion preservation solution for isolated liver

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