A kind of nano cartilage repair material with oriented nanofiber mat as skeleton and preparation method thereof

A nanofiber and cartilage repair technology, applied in medical science, prosthesis, etc., can solve the problems of poor mechanical properties of natural biomaterials, rapid degradation and absorption, and difficulty in mass production, and achieve good biocompatibility and Bioactivity, good biocompatibility, and good biodegradability

Inactive Publication Date: 2017-11-24
ZHONGYUAN ENGINEERING COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mechanical properties of natural biomaterials are poor, the degradation and absorption are too fast, and it is difficult to achieve mass production

Method used

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  • A kind of nano cartilage repair material with oriented nanofiber mat as skeleton and preparation method thereof
  • A kind of nano cartilage repair material with oriented nanofiber mat as skeleton and preparation method thereof

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0025] A preparation method of a nano-cartilage repair material with an oriented nano-fiber mat as a skeleton, the steps are as follows:

[0026] (1) Ultrasonic disperse graphene into hexafluoroisopropanol solution, then put silk fibroin and stir at room temperature for 48 hours to obtain a silk fibroin solution with a silk mass concentration of 20%, put hyaluronic acid into formic acid solution and stir at room temperature 48h, to obtain a hyaluronic acid solution with a mass concentration of 0.5%, pour the mixed solution of silk fibroin and graphene into the hyaluronic acid solution and stir for 24h at room temperature to obtain a spinning solution of silk fibroin / hyaluronic acid / graphene;

[0027] (2) The silk fibroin / hyaluronic acid / graphene mixed solution obtained in step (1) was electrospun, and the rotating drum was used as a receiving device to obtain an oriented nanofiber mat. The spinning voltage was 18 kV, and the spinning The total flow rate of the solution is 0.58...

Embodiment 2

[0032] A preparation method of a nano-cartilage repair material with an oriented nano-fiber mat as a skeleton, the steps are as follows:

[0033] (1) Ultrasonically disperse graphene into hexafluoroisopropanol solution, then put silk fibroin and stir at room temperature for 24 hours to obtain a silk fibroin solution with a silk mass concentration of 20%, put hyaluronic acid into formic acid solution and stir at room temperature 24h, to obtain a hyaluronic acid solution with a mass concentration of 0.15%, pour the mixed solution of silk fibroin and graphene into the hyaluronic acid solution and stir for 12h at room temperature to obtain a spinning solution of silk fibroin / hyaluronic acid / graphene;

[0034] (2) Electrospinning the silk fibroin / hyaluronic acid / graphene mixed solution obtained in step (1), using a rotating drum as a receiving device to obtain an oriented nanofiber mat. The spinning voltage was 14kV, the total flow rate of the spinning solution was 0.8mL / h, the inn...

Embodiment 3

[0038] A preparation method of a nano-cartilage repair material with an oriented nano-fiber mat as a skeleton, the steps are as follows:

[0039] (1) Ultrasonically disperse graphene into hexafluoroisopropanol solution, then put silk fibroin and stir at room temperature for 30 hours to obtain a silk fibroin solution with a silk mass concentration of 18%, put hyaluronic acid into formic acid solution and stir at room temperature 30h, to obtain a hyaluronic acid solution with a mass concentration of 0.5%, pour the mixed solution of silk fibroin and graphene into the hyaluronic acid solution and stir for 18h at room temperature to obtain a spinning solution of silk fibroin / hyaluronic acid / graphene;

[0040] (2) Electrospinning the silk fibroin / hyaluronic acid / graphene mixed solution obtained in step (1), using a rotating drum as a receiving device to obtain an oriented nanofiber mat. The spinning voltage was 18 kV, the total flow rate of the spinning solution was 0.56mL / h, the in...

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Abstract

The invention relates to a nano cartilage repair material taking an aligned nanofiber mat as a skeleton and a preparation method of the nano cartilage repair material. The nano cartilage repair material is prepared by preparing the aligned nanofiber mat containing graphene for being used as the skeleton through an electrospinning technique, casting a mixed solution containing fibroin, hyaluronic acid, chondroitin sulfuric acid and hydroxyapatite particles, and preparing the material through a crosslinking technique and a freeze drying device. The preparation method specifically comprises the steps of preparing a mixed solution containing fibroin and hyaluronic acid of the grapheme for being used as a spinning solution, electrostatically spinning to obtain the aligned nanofiber mat for being used as the skeleton, casting the mixed solution containing the fibroin, the hyaluronic acid, the chondroitin sulfuric acid and the hydroxyapatite particles after being stabilized through glutaraldehyde steam crosslinking, then carrying out a cross-linking reaction at the temperature of minus 20 DEG C to minus 40 DEG C by using a cross-linking agent, and preparing through freeze drying at the temperature of minus 54 DEG C. The nano cartilage repair material taking the aligned nanofiber mat as the skeleton, prepared by the invention, simulates a natural bone in the aspect of ingredient and structure, has excellent mechanical property, reasonable poriness and good biocompatibility and biological activity, can be used as the repair or substitute material for cartilage tissues, and has wide application prospect.

Description

technical field [0001] The invention relates to a nano-cartilage repair material with an oriented nano-fiber mat as a skeleton and a preparation method thereof, which is applied to a cartilage tissue repair material, and specifically relates to the technical field of biomedical materials. Background technique [0002] The difficulty in the treatment of cartilage defects caused by trauma, tumors, tuberculosis and metabolic diseases is one of the problems to be solved urgently in orthopedics. Cartilage is composed of cartilage tissue and the surrounding perichondrium. There are no blood vessels or nerves in cartilage tissue in adults, so the ability of cartilage tissue to repair itself after injury is limited. The traditional methods for repairing cartilage defects include: autologous tissue transplantation, allogeneic tissue transplantation, etc. However, the source of these tissues is limited, there is immune rejection, and the repair of cartilage defects cannot be long-term...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/48A61L27/56A61L27/58
Inventor 何建新谭卫琳韩啟明杨勇王利丹连艳平崔世忠丁彬
Owner ZHONGYUAN ENGINEERING COLLEGE
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