Bacillus cereus ly05 for efficiently degrading secbutaline and its application and use method

The technology of Bacillus cereus and fenbuterol, which is applied in the field of microorganisms, can solve the problems of bioremediation of pesticide residue pollution, etc., and achieve the effects of protecting the ecological environment and human health, being convenient to use and having low production costs.

Active Publication Date: 2019-03-05
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the diversity of pesticide structures, microbial degradation of pesticides is often highly targeted, resulting in the existing resource pool of degrading bacteria far from meeting the actual needs of bioremediation of pesticide residue pollution.
At present, there are no degrading microorganisms and related degrading preparations specifically for Zhongbuling

Method used

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  • Bacillus cereus ly05 for efficiently degrading secbutaline and its application and use method
  • Bacillus cereus ly05 for efficiently degrading secbutaline and its application and use method
  • Bacillus cereus ly05 for efficiently degrading secbutaline and its application and use method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 of the present invention: Isolation and identification of the butyrin-degrading bacteria LY05

[0027] 1) Isolation and screening of butylin-degrading bacteria LY05

[0028] Weigh 10 g of the long-term polluted soil with butylamine into a 250 mL conical flask, add 100 mL of inorganic salt medium, add butylline to a concentration of 100 mg / L, and place the conical flask on a shaker (30 Cultivate at 200 rpm for 7 days, inoculate 5 mL of the culture solution into fresh basal medium (100 mg / L secbutamyl), then culture in shake flask at 30°C for 7 days, and so on, press 100 mg secbutaline / L concentration gradient increases, so that the concentration of secbutaline in the final enrichment medium reaches 1000 mg / L, and the enrichment degradation strain is obtained.

[0029] During the enrichment and separation of degrading bacteria, after every 2 transfers, the bacterial suspension was prepared into 10 -2 ~10 -6 Serial dilutions were separated on the solid separa...

Embodiment 2

[0045] Embodiment 2 of the present invention: the preparation of Zhongbuling pesticide residue degradation bacterial agent:

[0046] Adopt the bacterial strain of bacillus cereus LY05 that efficiently degrades butyrin to produce the method for degrading butyrin preparation, comprising the steps:

[0047] 1) Cultivate the strain of Bacillus cereus LY05 in an inorganic salt medium at 30-35°C and 150-200 rpm to the logarithmic growth phase to obtain strains;

[0048] 2) Inoculate the above-mentioned strains into the seed bottle with an inoculum amount of 10% of the volume ratio of the inorganic salt medium, and vibrate at 30-35°C and 150-200 rpm until the logarithmic growth phase to obtain the seed liquid;

[0049] 3) Inoculate the obtained seed liquid into the fermentation medium according to the inoculation amount of 10% by volume, and ferment and cultivate it at 30-35°C and 150-200 rpm for 45-50h to obtain a fermentation liquid, the number of bacteria in the fermentation liqui...

Embodiment 3

[0051] Embodiment 3 of the present invention: Degradation bacteria LY05 is to the degradative performance of butaline:

[0052] Add secbutaline in the inorganic salt medium (same as Example 1) so that the final concentration is 50.0 mg / L; the inoculation concentration is 2.0×10 8 cfu / mL bacterial agent, and set the culture medium without inoculation as the control, culture in the dark at 30°C (200 rpm) for 0-7 days, and take samples regularly. The above-mentioned HPLC method was used to measure the residual amount of butaline and calculate the degradation rate. Degradation rate = (residual amount of control sample - residual amount of treated sample) / residual amount of control sample × 100%. see results figure 2 shown. The results showed that the degrading bacteria LGY06 could effectively degrade Zhongbuling in a short period of time, and the degradation rates of Zhongbuling reached 69.88% within 3 days, 85.84% within 5 days, and 85.84% within 7 days. The degradation rat...

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Abstract

The invention discloses a Bacillus cereus LY05 that efficiently degrades Zhongbuling and its application and use method. The strain has been preserved in the China Center for Type Culture Collection (CCTCC) on June 19, 2015. , the deposit number is CCTCC NO: M 2015397. The bacterial strain can effectively degrade the pesticide residue of secbutamyl in a short time. The bacterial agent prepared by the bacterial strain has the advantages of low production cost, convenient use, good removal effect and the like. The use of this bacterial agent can effectively repair the water and soil polluted by azoxystrobin-butadiene, solve the problem of excessive pesticide residues and environmental pollution in agricultural production, and protect the ecological environment and human health.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bacillus cereus LY05 capable of efficiently degrading secbutyrin and its application and usage method. Background technique [0002] The chemical name of Butralin is N-sec-butyl-4-tert-butyl-2,6-dinitroaniline, also known as Butralin, and its basic physical and chemical properties are shown in Formula 1. Zhongbuling belongs to dinitroaniline herbicides, which can be absorbed by weed roots or bud sheaths. After the agent enters the plant, it mainly inhibits the cell division of meristems, thereby inhibiting the growth of weed sprouts and young roots. It can be used for Cotton, soybeans, peas, rapeseed, peanuts, potatoes, winter wheat, barley, castor, sunflower, sugar cane, vegetables, fruit trees, etc., mainly used to control monocotyledonous weeds and annual broad-leaved weeds, and can also control small-seeded twins leaf weeds. It can also be used as a plant growth re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02B09C1/10C02F3/34C12R1/085A62D101/04A62D101/28C02F101/38
CPCA62D3/02A62D2101/04A62D2101/28B09C1/10C02F3/34C02F2101/38C12N1/205C12R2001/085
Inventor 吴小毛张承李荣玉龙友华李明尹显慧
Owner GUIZHOU UNIV
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