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Gelatin and polyester copolymerized composite biological material and preparation method thereof

A technology of composite biomaterials and copolymers, applied in the field of gelatin/polyester copolymerized composite biomaterials and its preparation, can solve problems such as poor hydrophilicity, lack of reactive side groups, and unsatisfactory cell affinity

Active Publication Date: 2015-11-04
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Biodegradable aliphatic polyesters are a class of synthetic biomaterials that have been widely studied and clinically applied. They have good biocompatibility and adjustable biodegradation speed, but generally lack reactive side groups and poor hydrophilicity. Cell affinity is also not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Dissolve 5g of gelatin in 40ml of trifluoroethanol, dissolve at 37°C for 12 hours, add 2.45ml of triethylamine and stir evenly; dissolve 4.9ml of 2-bromoisobutyryl bromide in 10ml of trifluoroethanol; Add the 2-bromoisobutyryl bromide solution dropwise to the gelatin trifluoroethanol solution, react in an ice-water bath for 2 hours, then raise the temperature of the system to 25°C and continue the reaction for 48 hours; after the reaction, use ethanol to precipitate and wash Purify and prepare ATRP macromolecular initiator brominated gelatin (Gel-Br-1), the atomic percent content of bromine is 2.76%; (2) 10g L-lactide, 1.2g cetyl alcohol, 0.005g iso Add stannous octoate to the polymerization tube, evacuate, and blow nitrogen to remove oxygen, repeat three times, each time for 30 minutes, then keep the polymerization tube in a vacuum state for fusion sealing, and put the polymerization tube at 140 ° C for 24 hours. The crude polymerization product was dissolved in ch...

Embodiment 2

[0019] Step (1) is the same as in Example 1; (2) Add 10g of L-lactide, 0.807g of cetyl alcohol, and 0.005g of stannous isooctanoate into the polymerization tube, vacuumize, and ventilate nitrogen to remove oxygen. Repeat three times, each time for 30 Minutes, and then keep the polymerization tube under the vacuum state to melt seal, put the polymerization tube at 140 ° C for 24 hours; dissolve the crude polymerization product in chloroform, use ethanol precipitation to remove unreacted monomer, and dry to obtain a monomer with a molecular weight of 3000 Hydroxyl-terminated poly-L-lactide (PLLA3000); dissolve 5g of PLLA3000 in 25ml of chloroform, add 0.62ml of triethylamine and 1.24ml of methacrylic anhydride, react under reflux for 48 hours, and purify by ethanol precipitation to prepare single-ended Double-bonded poly-L-lactide (PLLA3000-MA); (3) Dissolve 0.2g Gel-Br-1 and 1.87g PLLA3000-MA in 50ml trifluoroethanol, mix well and add 1,1,4,7 , 13 μl of 7-pentamethyldiethylenet...

Embodiment 3

[0021] (1) Dissolve 5g gelatin in 40ml trifluoroethanol, dissolve at 37°C for 12 hours, add 0.74ml triethylamine and stir evenly; dissolve 1.47ml 2-bromoisobutyryl bromide in 10ml trifluoroethanol; 2-bromoisobutyryl bromide solution was added dropwise into the gelatin trifluoroethanol solution, and reacted in an ice-water bath for 2 hours, then the system was heated to 37°C and continued to react for 24 hours. After the reaction, use ethanol precipitation, washing and purification to prepare ATRP macroinitiator brominated gelatin (Gel-Br-2), the atomic percentage of bromine is 0.89%; (2) 10g L-lactide, Add 0.242 hexadecanol and 0.005g of stannous isooctanoate into the polymerization tube, vacuumize, blow nitrogen to remove oxygen, repeat three times, each time for 30 minutes, and then keep the polymerization tube in a vacuum state for fusion sealing, and put the polymerization tube at 140°C for reaction 24 hours. Dissolve the crude polymerization product in chloroform, use et...

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Abstract

The invention relates to a biological material capable of being used for tissue regenerative repair, in particular to a gelatin and polyester copolymerized composite biological material and a preparation method thereof. The gelatin and polyester copolymerized composite biological material and the preparation method thereof are characterized in that biodegradable aliphatic polyester with different chemical compositions and different chain lengths is grafted to extracellular matrix protein molecule main chains through a side group chemical reaction to obtain the gelatin and polyester copolymerized composite biological material capable of being used for the tissue regenerative repair. The preparation method of the gelatin and polyester copolymerized composite biological material comprises the technical steps that 1, the gelatin reacts with bromacyl bromide compounds to obtain bromide gelatin with different bromine substituted degrees, and the bromide gelatin serves as a macromolecular initiator for atom transfer radical polymerization; 2, ring opening polymerization is conducted on lactone monomers to obtain single-ended hydroxyl polyester with different molecular weights, and the single-ended hydroxyl polyester reacts with methacrylic anhydride to obtain single-ended double-bond polyester; 3, the bromide gelatin reacts with the single-ended double-bond polyester to obtain a gelatin grafted polyester copolymer finally. According to the gelatin and polyester copolymerized composite biological material and the preparation method thereof, the hydrophilic-hydrophobic property and the biodegradability of the gelatin grafted polyester copolymer can be controlled by changing the chemical compositions, the chain lengths and the grafting degrees of the polyester side chains, wherein according to the hydrosoluble gelatin grafted polyester copolymer, physical crosslinking of the gelatin can be achieved through stereocomplex interaction or hydrophobic interaction of the polyester side chains.

Description

technical field [0001] The invention relates to a biological material that can be used for tissue regeneration and repair, in particular to a gelatin / polyester copolymerized composite biological material and a preparation method thereof. Background technique [0002] Natural extracellular matrix proteins such as collagen and elastin are important regulators of the extracellular microenvironment, so they are attractive scaffold materials for tissue engineering. Due to the hydrophilic nature of protein materials, scaffolds prepared from them generally require cross-linking to reduce the dissolution or swelling of the materials, thereby maintaining scaffold mechanical and structural stability. Although the stability of the protein scaffold material obtained by cross-linking with chemical cross-linking agents is improved, the toxicity of small molecule chemical reagents will adversely affect the cells and the loaded biologically active substances, as well as the diffusion of cro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G81/00C08G63/91C08G63/08C08H1/00A61L27/24
Inventor 蔡晴王杨任志伟杨小平
Owner BEIJING UNIV OF CHEM TECH
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