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Novel concentrating and continuous isoelectric extraction technology for glutamic acid

A technology for concentrating continuous isoelectricity and glutamic acid, applied in water/sludge/sewage treatment, biochemical equipment and methods, microorganisms, etc., can solve the problems of low fermentation efficiency, large wastewater volume, and high concentration of glutamic acid problems, to achieve the effect of increasing industrial added value, low consumption and enhanced effect

Inactive Publication Date: 2015-11-11
HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantage of this process is that the extraction yield is high, and the general total yield can reach 94%, but this process produces an additional 40% of high-concentration wastewater, and consumes an additional 100% of sulfuric acid and 45% of liquid ammonia
[0004] The defects in the glutamic acid process are mainly the low fermentation efficiency, the large amount of wastewater produced, and the high concentration. Overcoming the above defects is the direction of our research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A new process for concentrated and continuous isoelectric extraction of glutamic acid, which includes the following steps:

[0032] 1) Prepare mixed seed solution

[0033] (a) Preparation of Corynebacterium glutamicum seed solution:

[0034] Corynebacterium glutamicum (Corynebacterium glutamicum) ATCC13032 is inserted in Corynebacterium glutamicum seed culture medium, inoculum size is 3%, at 30 DEG C, cultivate 18h in 180rpm shaker, the Corynebacterium glutamicum seed liquid that obtains; The components of the Corynebacterium acidic acid seed culture medium are (the following are mass percentages): glucose 2.5%, corn steep liquor 3%, K 2 HPO 4 0.2%, Na 2 HPO 4 0.2%, biotin 0.5%, MgSO 4 0.2%, 0.025% urea, sterilized at 115°C for 15 minutes;

[0035] (b) Preparation of Brevibacterium lactose fermentum seed solution:

[0036] Brevibacterium lactofermentum (Brevibacterium lactofermentum) ATCC13869 was inoculated into the seed medium of Brevibacterium lactofermentum, ...

Embodiment 2

[0054] 1. Strain fermentation comparison test (same fermentation conditions): the fermentation strain uses Corynebacterium glutamicum ATCC13032, and the highest glutamic acid production can reach 200g / L (see the applicant's previous patent cn201210211393 ); while using the mixed bacterial strain of the present invention to ferment, the output of glutamic acid reaches 221g / L, which increases by 10.5%, which is much better than single bacterial strain fermentation.

[0055] 2. Examples of wastewater treatment effects

[0056] Take Fufeng production workshop, extract waste water from glutamic acid, treat waste water according to the method of embodiment 1, take samples to measure COD, ammonia nitrogen data;

[0057] Compared with Example 1, the control 1 group did not add liquid bacterial agent; compared with Example 1, the control 2 group had only wheat bran as the adsorbent carrier. Sampling and determination of COD, ammonia nitrogen, and total nitrogen data, the test data ar...

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PUM

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Abstract

The invention belongs to the technical field of food, and discloses a novel concentrating and continuous isoelectric extraction technology for glutamic acid. The technology comprises the following steps of 1 mixed seed liquid preparing; 2 glutamic acid preparing through fermentation; 3 concentrating and continuous isoelectric focusing; 4 waste liquid treating. According to the technology, the fermentation efficiency can be improved, waste water can be effectively treated, environmental protection can be achieved, and pollution does not exist.

Description

technical field [0001] The invention belongs to the technical field of food, and in particular relates to a new process for concentrated and continuous isoelectric extraction of glutamic acid. Background technique [0002] Glutamic acid is an acidic amino acid. The molecule contains two carboxyl groups, and its chemical name is α-aminoglutaric acid. Glutamic acid was discovered by Li Suxun in 1856. It is a colorless crystal with umami taste, slightly soluble in water, but soluble in hydrochloric acid solution, with an isoelectric point of 3.22. A large amount exists in cereal protein, and the content in animal brain is also more. Glutamic acid plays an important role in the protein metabolism process in organisms, and participates in many important chemical reactions in animals, plants and microorganisms. MSG is prepared from glutamic acid. [0003] The existing technologies for extracting glutamic acid from glutamic acid fermentation broth mainly include: isoelectric ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P13/14C07C229/24C07C227/40C02F3/34C12R1/01C12R1/15
Inventor 刘世周程士清王刚邱云
Owner HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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