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Complex microbial community and application thereof in alcohol production by syngas fermentation

A technology of compound flora and syngas, applied in the fields of fermentation, bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, etc. Pollution and other problems, to achieve the effect of wide source of raw materials and good stability

Inactive Publication Date: 2015-11-25
安徽科灵环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such harsh conditions are difficult to achieve in the process of large-scale industrial production
[0005] Therefore, the problem that Clostridium is easily polluted and has strict requirements for anaerobic environment should be further solved

Method used

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  • Complex microbial community and application thereof in alcohol production by syngas fermentation
  • Complex microbial community and application thereof in alcohol production by syngas fermentation
  • Complex microbial community and application thereof in alcohol production by syngas fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Embodiment 1 The device provided by the present invention

[0088] The device provided by the present invention is as figure 1 , comprising: a main reactor (1), a branch reactor (2), a syngas supply device (3), a culture medium supply device (4), a reactant recovery device (5) and a reaction system pH automatic control device (6 );

[0089] The main reactor (1) and the branch reactor (2) communicate through the first pipeline (7) and the second pipeline (8); the culture medium supply device (4) is set on the first pipeline; the second A reactant recovery device (5) and a reaction system pH automatic control device (6) are arranged on the pipeline; the synthesis gas supply device (3) communicates with the main reactor (1) through the third pipeline (9) and passes through the fourth pipeline (10) communicates with branch reactor (2).

[0090] In order to facilitate the observation of fermentation conditions, the shell of the branch reactor is made of glass, and the she...

Embodiment 2

[0099] Embodiment 2 flora screening

[0100] The composition of the medium used in this embodiment is: NH 4 Cl530mg / L; KH 2 PO 4 200mg / L; Na 2 SO 4 50mg / L; KCl50mg / L; CaCl 2 10mg / L; MgCl 2 . 6H 2 O70mg / L; MnCl 2 . 4H 2 O0.8mg / L; CoCl 2 . 2H 2 O1.2mg / L; FeSO 4 . 7H 2 O3.2mg / L; AlCl 3 0.5mg / L; NaMO 4 . 2H 2 O0.1mg / L; H 3 BO 3 0.2mg / L; NiCl 2 . 6H 2 O0.5mg / L; CuCl 2 . 2H 2 O1.1mg / L; ZnSO 4 . 2H 2 O3.2mg / L; EDTA-2Na3.0mg / L; biotin 2.0mg / L; folic acid 2.0mg / L; vitamin B610mg / L; riboflavin 5.0mg / L; vitamin B15.0mg / L; niacin 5.0 mg / L; pantothenic acid 5.0mg / L; vitamin B120.1mg / L; p-aminobenzoic acid 5.0mg / L; lipoic acid 5.0mg / L.

[0101] Using the device of Example 1, mix the anaerobic sludge with the culture medium (the mass ratio of the sludge to the culture medium is 5:95), inject it into the fermentation device to make the liquid reach the water outlet, and open the pressure stabilizing valve to adjust the air pressure to 0.01 MPa~0.02Mpa, open al...

Embodiment 3

[0107] Embodiment 3 syngas fermentation

[0108] The composition of the medium used in this embodiment is: NH 4 Cl530mg / L; KH 2 PO 4 200mg / L; Na 2 SO 450mg / L; KCl50mg / L; CaCl 2 10mg / L; MgCl 2 . 6H 2 O70mg / L; MnCl 2 . 4H 2 O0.8mg / L; CoCl 2 . 2H 2 O1.2mg / L; FeSO 4 . 7H 2 O3.2mg / L; AlCl 3 0.5mg / L; NaMO 4 . 2H 2 O0.1mg / L; H 3 BO 3 0.2mg / L; NiCl 2 . 6H 2 O0.5mg / L; CuCl 2 . 2H 2 O1.1mg / L; ZnSO 4 . 2H 2 O3.2mg / L; EDTA-2Na3.0mg / L; biotin 2.0mg / L; folic acid 2.0mg / L; vitamin B610mg / L; riboflavin 5.0mg / L; vitamin B15.0mg / L; niacin 5.0 mg / L; pantothenic acid 5.0mg / L; vitamin B120.1mg / L; p-aminobenzoic acid 5.0mg / L; lipoic acid 5.0mg / L.

[0109] Using the device provided in Example 1, mix the flora screened in Example 2 with the culture medium (the mass ratio of the flora to the culture medium is 5:95), inject it into the fermentation device so that the liquid position reaches the water outlet, and open the pressure stabilizing valve Adjust the air pressur...

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Abstract

The invention relates to the field of fermentation engineering, in particular to a complex microbial community and application thereof in alcohol production by syngas fermentation. The complex microbial community is high in stability and alcohol production efficiency, and is free of infectious microbes contamination within 30 days of continuous fermentation; by using the complex microbial community in syngas biological fermentation, alcohol can be produced stably and abundantly; alcohol concentration in fermentation broth is up to 17.2 g / L, and alcohol in a fermentation product is up to 92% in mass percent.

Description

technical field [0001] The invention relates to the field of fermentation engineering, in particular to a complex bacterial flora and its application in synthesis gas fermentation to produce alcohol. Background technique [0002] At present, the uneven distribution and single structure of energy have become one of the bottlenecks restricting my country's sustainable development. The problem of how to convert energy with low energy density into energy with high energy density and easy storage and transportation needs to be solved urgently. Syngas is a mixed gas produced by partial oxidation and pyrolysis of biomass such as lignocellulose, and its main components are CO and H 2 , including a small amount of CO 2 、CH 4 、H 2 S and NO X Wait. Syngas has low energy density and is not easy to store and transport. Generally, useful chemicals such as acetic acid and ethanol can be generated by Fischer-Tropsch synthesis technology. Compared with syngas, liquid useful chemicals (s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/06C12M1/36C12M1/04C12R1/145C12R1/225C12R1/01
CPCY02E50/10
Inventor 曾建雄夏修阳王云琦
Owner 安徽科灵环境科技有限公司
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