10 results about "Syngas fermentation" patented technology

This invention belongs to the field of bioengineering and carbon one-carbon resource utilization technology, specifically relating to a bacterial strain that produces acetic acid and acetate through syngas fermentation, as well as its applications and methods. The strain is *Clostridium yongdarii* (…). Clostridium ljungdahlii SL40, deposited at the Guangdong Provincial Microbial Culture Collection Center (GDMCC No. 67679) on January 15, 2026, represents a strain that achieves highly efficient H2 / CO2 conversion in syngas, with a maximum CO2 utilization rate of 1.8 g / L / h. The anaerobic fermentation process produces zero pollutants, enabling resource utilization and carbon reduction of industrial waste gas, and providing a new technological pathway for carbon resource conversion.

The present invention relates to a system for producing an organic acid from a syngas fermentation product using a bipolar electrodialysis device. By combining syngas fermentation using microorganisms and bipolar electrodialysis, the extraction of organic acid precursors and the production of organic acids can be performed in a single process. In addition, a base produced in the bipolar electrodialysis device can be reused in the microbial fermentation process to adjust the pH of the medium, thereby improving the efficiency of microbial fermentation.

The invention relates to a synthesis gas fermentation clarified liquor sterilization system and method. The system comprises a clarified liquor preheating unit (1), a clarified liquor heating sterilization unit (2) and a clarified liquor flash evaporation unit (3), the clarified liquor preheating unit (1), the clarified liquor heating and sterilizing unit (2) and the clarified liquor flash evaporation unit (3) are sequentially arranged along the flowing direction of the synthesis gas fermentation clarified liquor, the clarified liquor heating and sterilizing unit (2) comprises a heater (21) and a sterilizer (22) which are sequentially arranged, and the clarified liquor flash evaporation unit (3) is connected with the clarified liquor preheating unit (1) through a flash steam pipeline. Compared with the prior art, the invention provides a process flow capable of continuously operating the clarified liquid sterilization and cooling process and reducing the CIP cleaning frequency of the heat exchanger, and the process flow has great significance in promoting the synthesis gas fermentation technology.

This application provides a system for the co-production of ethanol and hexanoic acid through syngas fermentation, belonging to the field of syngas fermentation. The system includes: a primary reactor through which feed gas is introduced; a microbial cell separation device, the inlet of which is connected to the outlet of the primary bioreactor; a distillation device, the inlet of which is connected to the clear liquid outlet of the microbial cell separation device; and a secondary reactor, the inlet of which is connected to the residual distillate outlet of the distillation device. This system enables primary fermentation to produce metabolites such as ethanol and acetic acid. After ethanol is extracted by distillation, acetic acid is used as a substrate in the secondary fermentation system to produce hexanoic acid. Therefore, this system avoids the acetic acid produced during primary fermentation from entering the downstream wastewater system, reducing the wastewater treatment load, and simultaneously converting low-value products into high-value products, thus improving economic efficiency.

The invention relates to a fermentation method for producing n-butyl alcohol by utilizing synthesis gas fermentation, which is characterized in that the n-butyl alcohol is produced by utilizing synthesis gas fermentation based on a Moorella thermoacetica strain, and in the fermentation process, the pH is controlled to be 5.5-7.0, and the oxidation-reduction potential is controlled to be-250mV to-200mV. Therefore, the fermentation yield of the n-butyl alcohol can be greatly improved, and the yield of the n-butyl alcohol in the fermentation liquid is 30 g / L or above. Moreover, the traditional process for producing n-butyl alcohol through synthesis gas fermentation has many limitations, such as low efficiency, high cost, many byproducts, poor product selectivity and the like. The problems limit the application of the synthesis gas fermentation technology in the production of biofuels and chemicals. In the application, an improved synthesis gas fermentation method is provided, and the yield and selectivity of n-butyl alcohol are remarkably improved by accurately controlling the pH and Redox (redox potential) conditions in the fermentation process.

The present application relates to the technical field of microbial fermentation, and particularly relates to a method for producing ethanol and / or single-cell protein by Clostridium fermentation of synthesis gas. The present application significantly improves the biomass and the yield of ethanol by supplementing nitrogen source and sulfur source and optimizing the supplementing mode during the process of ethanol production by Clostridium fermentation of synthesis gas, so that ethanol becomes the main product of fermentation. The fermentation production method provided by the present application has the advantages of high efficiency, high yield, high proportion of ethanol in fermentation products, high biomass, simple process and easy operation, and has a good application prospect in the production of ethanol by synthesis gas fermentation, and provides a new means and method for efficient synthesis of ethanol and single-cell protein by synthesis gas.

The invention relates to a syngas fermentation broth cooling system and method.The system comprises a circulating discharging unit (2) and a circulating feeding unit (3) which are connected with a biological reaction unit (1), the circulating feeding unit (3) comprises a circulating pipe A (31) and a circulating pipe B (32), the inlet end of the circulating pipe A (31) is connected with the circulating discharging unit (2), and the outlet end of the circulating pipe A (31) is connected with the biological reaction unit (1); the inlet end of the circulating pipe B (32) is connected with the circulating discharge unit (2), and the outlet end is connected with the biological reaction unit (1) through the heat exchange unit (4). Compared with the prior art, the method has the advantages that the fermentation activity of the synthesis gas biological reaction unit can be continuously and stably operated, the cleaning frequency of the heat exchanger can be reduced, or even if the heat exchanger is cleaned, the fermentation activity cannot be impacted, and the method has great significance in promoting the development of a synthesis gas fermentation technology.

This invention belongs to the field of microbial and genetic engineering technology, specifically relating to a genetically engineered *C. yongdar* strain and its application in the synthesis of ethanol, lactic acid, and 2,3-butanediol from one-carbon gas fermentation. This invention proposes a strategy for metabolic modification of *C. yongdar* based on the lactate cycle. Three engineered strains were constructed by knocking out lactate dehydrogenase (LDH) producing lactate, knocking out lactate dehydrogenase oxidizing lactate, and overexpressing LDH producing lactate dehydrogenase based on the knockout of lactate dehydrogenase oxidizing lactate. This modification strategy can increase the yield of 2,3-butanediol, ethanol, and lactic acid in the one-carbon gas autotrophic fermentation of *C. yongdar*, providing a feasible metabolic modification technology for the syngas fermentation of acetic acid-producing bacteria to produce high-value compounds, thus possessing good practical application value.

The invention discloses an ethanol-producing clostridium engineering strain for producing ethanol and acetic acid as well as a construction method and application of the ethanol-producing clostridium engineering strain, and belongs to the field of synthetic biology and metabolic engineering. Aiming at the technical problems of limited energy metabolism, slow growth and low product yield of the clostridium ethanologenes in the synthesis gas fermentation process, the invention adopts a push-pull-resistance synergistic metabolism reprogramming strategy: firstly, knocking out by-product synthesis genes budA and ldhA to block carbon shunt; then integrating and expressing endogenous transcription factors CLAURS14020 and CLAURS08380, which are subjected to energy stress induction screening, and a pyruvate-phosphate double kinase PPDK coding gene at the knockout site, and dragging a carbon metabolic flux, wherein the endogenous transcription factors CLAURS14020 and CLAURS08380 are subjected to energy stress induction screening; and finally, introducing an energy metabolism enhancement gene Rnf complex to enhance cell energy supply. The engineering strain constructed by the invention can efficiently utilize the synthesis gas as a carbon source, obviously improves the yield of ethanol and acetic acid, and is suitable for green manufacturing of biofuels and chemicals.

The present application relates to the technical field of microbial fermentation, and particularly relates to a method for producing acetic acid by fermentation of synthesis gas. The present application provides a method for producing acetic acid by fermentation of synthesis gas, which comprises: producing acetic acid by fermentation of synthesis gas by Clostridium, and controlling the pH of the fermentation system by using an alkali liquor containing a nitrogen source and a sulfur source during the fermentation process. The method significantly improves the yield of acetic acid produced by Clostridium fermentation of synthesis gas by optimizing the supplementing mode of the nitrogen source and the sulfur source during the fermentation process, and has the advantages of high acetic acid synthesis efficiency, high yield, simple process and easy operation, and has a good application prospect in the fermentation of synthesis gas to produce acetic acid.