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Composite bacterial enzyme system with multiple effects and application thereof

A compound bacterial enzyme and system technology, applied in the direction of application, fungicide, organic fertilizer, etc., can solve the problems of unfavorable agricultural sustainable development, soil damage, soil salinization, etc., to improve plant disease resistance, prevent and control plant diseases and insect pests , the effect of promoting plant growth

Active Publication Date: 2015-12-16
潘灿平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such a method will cause a large amount of chemicals to enter the farmland soil, causing serious damage to the soil, causing a sharp decline in the quality of the farmland soil, and further highlighting problems such as soil salinization, pesticide residues, and heavy metal pollution, which is not conducive to The sustainable development of agriculture will also cause a series of problems such as excessive pesticide residues and a decline in the quality of agricultural products.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1, the preparation of the different formulations of compound bacterial enzyme system

[0059] 1. Activation and expansion of bacteria

[0060] Activation: Inoculate Bacillus subtilis on No. 1 medium and culture in a 30°C incubator for 3 days to obtain activated Bacillus subtilis; inoculate Pediococcus lactis on No. 3 medium and culture in a 30°C incubator After 3 days, activated Pediococcus lactis was obtained.

[0061] Expanded cultivation: the above-mentioned activated Bacillus subtilis and activated Pediococcus lactis were respectively inserted into two fermenters equipped with No. 1 medium and No. 2 medium respectively, and both were statically expanded step by step at 30°C. The inoculum size is 5%-10% (volume percentage content) in the expansion culture process, obtains the Bacillus subtilis fermented liquid (1 * 10 9 cfu / ml) and Pediococcus lactis fermentation broth (1×10 9 cfu / ml).

[0062] 2. Preparation of complex bacterial enzyme system water an...

Embodiment 2

[0067] Embodiment 2, compound bacterial enzyme system can improve the output and disease resistance of melon

[0068] The design of the field experiment is as follows: the experiment adopts a randomized block design, and 3 replicated plots are set up, and each replicated plot is randomly set up with 2 treatment plots, which are the powder treatment plot and the control treatment plot. The area of ​​each treatment area is 0.5 mu, and each treatment area has 10 muskmelons (Cucumismelo) 15 days after emergence.

[0069] In this embodiment, the soil humidity in the field experiment is 16%-18%, and the air temperature is 20-30°C.

[0070] 1. Powder processing area

[0071] The powder treatment area is carried out as follows: the composite bacteria enzyme system powder suspension (the composite bacteria enzyme system powder suspension is the suspension obtained by adding the composite bacteria enzyme system powder of 200g embodiment 1 to 150 liters of water) root irrigation applica...

Embodiment 3

[0085] Embodiment 3, compound bacterial enzyme system can improve the yield and disease resistance of tomato

[0086] The design of the field experiment is as follows: the experiment adopts a randomized block design, and 3 replicated plots are set up, and each replicated plot is randomly set up with 2 treatment plots, which are the powder treatment plot and the control treatment plot. The area of ​​each treatment area is 0.5 mu, and each treatment area applies organic fertilizer according to the application method of conventional organic manure in 4 days before the planting of tomato (Lycopersicone sculentum) seedlings, and plows the ground; 2 days before the field planting of tomato (Lycopersicone sculentum) Add 150 g of complex bacterial enzyme system powder into 60 L of warm water and let it stand for 12 hours to activate the agent to obtain an activated powder aqueous solution.

[0087] In this embodiment, the soil humidity in the field experiment is 16%-18%, and the air t...

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Abstract

The invention discloses a composite bacterial enzyme system with multiple effects and application thereof. The composite bacterial enzyme system provided by the invention is composed of a microbial agent and an organic matter, wherein active ingredients of the microbial agent are composed of Bacillus subtilis CGMCC No.1.1630 and Pediococcus acidilactici CGMCC No.1.2696; and the organic matter is humic acid. Experimental results show that the composite bacterial enzyme system provided by the invention can regulate plant growth, increase plant yield, prevent and control plant diseases and pests, enhance plant disease resistance, improve soil and enhance soil fertility.

Description

technical field [0001] The invention relates to a compound bacterial enzyme system with multiple effects and its application in the field of biotechnology. Background technique [0002] Modern agriculture pays more and more attention to the comprehensive effect of agricultural preparations (such as the development of modern fungicides, the promotion effect on crop growth and development has also become an important aspect of evaluating the effect of fungicides), so as to provide comprehensive and comprehensive protection for crops and ensure crops. good growth. The agrochemicals used in modern agriculture are generally "special prevention and treatment", which makes agriculture apply a large number of various chemicals at each growth stage of crops to ensure that crops are fully protected. protection. However, such a method will cause a large amount of chemicals to enter the farmland soil, causing serious damage to the soil, causing a sharp decline in the quality of the fa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P21/00A01P3/00A01P7/00C09K17/00C05G3/00C09K101/00
Inventor 潘灿平李子昂潘弦李雪生冉成中
Owner 潘灿平
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