Fluorescence probe for identifying hydroxyl radicals rapidly and specifically as well as preparation method and application of fluorescence probe
A technology of fluorescent probes and free radicals, applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of difficulty in achieving long-wavelength excitation, susceptibility to interference from external factors, and reduced practicability, etc., to achieve Strong anti-interference ability of other molecules, simple post-processing process, and easy operation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0023] Embodiment 1: the synthesis of fluorescent probe CCy-OH
[0024] The synthetic route is as follows:
[0025]
[0026] 1) Compound 1 (0.2 mmol, 1.0 equiv) and compound 2 (0.2 mmol, 1.0 equiv) were added to 1.0 mL of ethanol solvent under nitrogen atmosphere, heated to reflux under nitrogen atmosphere, and reacted for 6 hours. The reaction was detected with a TCL plate. After the reaction was complete, it was concentrated and subjected to column chromatography to obtain compound 3 with a yield of 90%.
[0027] 2) Compound 3 (0.1 mmol, 1.0 equiv) was dissolved in MeOH (2.0 mL) and stirred at 0°C, sodium borohydride (0.5 mmol, 5 equiv) was added dropwise to the above solution in batches, and reacted at room temperature for 5 h. The reaction was detected with a TCL plate. After the reaction was complete, it was extracted with DCM, dried over anhydrous sodium sulfate, concentrated, and subjected to column chromatography. The probe CCy-OH could be obtained with a yield of ...
Embodiment 2
[0028] Embodiment 2: Probe CCy-OH changes with the increase of the addition equivalent of · OH fluorescence spectrogram
[0029] The CCy-OH fluorescent probe prepared in Example 1 was dissolved in DMF to prepare a 1 mmol / L stock solution. Take 30μL from the stock solution and add it to a 5mL centrifuge tube, and add different equivalents . OH standard solution was diluted to 3 mL (10 μM) with DMF / PBS (1:1, v / v), and its fluorescence properties were measured. Fluorescence spectra such as figure 2 shown. Depend on figure 2 It can be seen that the fluorescence intensity of CCy-OH gradually increases with the increase of the addition of OH, and when it increases to a certain extent, the fluorescence intensity decreases instead, mainly because the generation of reactive oxygen species can bleach the probe.
Embodiment 3
[0030] Embodiment 3: The change of probe CCy-OH to the fluorescence spectrogram that changes with time
[0031] Take 30 μL from the fluorescent probe stock solution in Example 2 and add it to a 5mL centrifuge tube, add OH (25equiv) standard solution, and use MeOH / H 2 O (1:1, v / v) was diluted to 3 mL (10 μM), and its fluorescence properties were measured. Fluorescence spectra such as image 3 shown. Depend on image 3 It can be seen that after adding the probe CCy-OH, the prepared OH can be added directly, and the fluorescence intensity can quickly reach the maximum within 0.5 min, realizing the rapid detection of OH.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More