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Polyporus seleno-polysaccharide preparation method

A technology of selenized polysaccharides and polyporus polysaccharides, which is applied in the field of traditional Chinese medicine processing, can solve the problems of low stability of selenized polyporus polysaccharides, large amount of selenizing agent used, long selenization reaction time, etc., and achieve selenization reaction time Short, high yield of selenized polysaccharides, the effect of less usage

Inactive Publication Date: 2015-12-30
刘瑛
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Problems solved by technology

The amount of selenizing agent used in the preparation of selenium polysaccharides in the above patents is large, the selenization reaction time is long, the selenium content is not high, and the prepared selenized polyporus polysaccharides have low stability and low biological potency

Method used

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  • Polyporus seleno-polysaccharide preparation method

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Embodiment 1

[0030] Preparation of Polyporus polysaccharide: Select clean Polyporus sclerotia without impurities, weigh 1kg, dry by microwave, pulverize with a pulverizer, pass through a 40-mesh sieve, add 20L of distilled water, stir evenly, ultrasonically treat for 5min, power 600W , under the conditions of neutral pH and 90°C, extract for 2.5 hours, filter and separate, put the extracted filtrate into a vacuum concentrator, concentrate to 2L, add 4.7L of absolute ethanol to the concentrated solution, and let it stand for 8 hours , centrifuged, and the precipitate was dried to obtain 312.4 g of polyporus polysaccharide.

[0031] Purification of Polyporus crude polysaccharide: Add the obtained Polyporus polysaccharide into 1.9L of distilled water, centrifuge for 10min, collect the clear liquid, add 28.5ml of 2% tannic acid solution dropwise to it while stirring, boil for 10min, add 28.5g of sugar for Decolorize activated carbon, stir well, decolorize for 10 minutes, filter while it is hot...

Embodiment 2

[0034] Preparation of Polyporus polysaccharide: Select clean Polyporus sclerotia without impurities, weigh 1kg, dry by microwave, pulverize with a pulverizer, pass through a 60-mesh sieve, add 30L of distilled water, stir evenly, ultrasonically treat for 8min, power is 650W , at a neutral pH and a temperature of 95°C, extract for 3 hours, filter and separate, put the extract filtrate into a vacuum concentrator, concentrate to 6L, add 18L of absolute ethanol to the concentrate, and make the ethanol in the mixture The concentration is 75%, let it stand for 10 hours, centrifuge, and dry the sediment to obtain 331.6 g of polyporus polysaccharide.

[0035]Purification of polyporus polysaccharides: add the obtained polysaccharides of polyporus polysaccharides into 2.7L of distilled water, centrifuge for 15 minutes, collect the clear liquid, add 54ml of 2% tannic acid solution dropwise to it while stirring, boil for 12 minutes, add 54g of decolorized activated carbon for sugar , stir...

Embodiment 3

[0038] Preparation of Polyporus polysaccharide: Select clean Polyporus sclerotia without impurities, weigh 1kg, dry in microwave, pulverize with a pulverizer, pass through 80-mesh sieve, add 40L of distilled water, stir evenly, ultrasonically treat for 10min, power is 700W , under the conditions of neutral pH and temperature of 100°C, extract for 3.5 hours, filter and separate to obtain the extract filtrate; put the extract filtrate into a vacuum concentrator, concentrate to 10L, add 40L of absolute ethanol to the concentrate , make the ethanol concentration in the mixed solution be 80%, let it stand for 10h, centrifuge, and dry the sediment to obtain 324.3g of polyporus polysaccharide.

[0039] Purification of Polyporus crude polysaccharide: add the obtained Polyporus polysaccharide into 3.2L of distilled water, centrifuge for 20min, collect the clear liquid, add 96ml of 2% tannic acid solution dropwise to it while stirring, boil for 15min, add 96g of decolorized activated car...

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Abstract

The invention discloses a polyporus seleno-polysaccharide preparation method and belongs to the technical field of traditional Chinese medicine processing. The polyporus seleno-polysaccharide preparation method comprises hydrolyzing a polyporus crude polysaccharide by papain, purifying the hydrolysate by an alumina purifying column to obtain refined polyporus pure polysaccharide, mixing the refined polyporus pure polysaccharide, dehydrated pyridine as a selenylation reaction initiator, selenium oxychloride, barium chloride as a catalyst and a sodium nitrate solution as a chemical stabilizing agent, and carrying out a reaction process to produce polyporus seleno-polysaccharide. The preparation method of the seleno-polysaccharide from polyporus crude polysaccharide has the advantages of fast and high efficiency operation, high product purity, small selenylation agent use amount, high seleno-polysaccharide yield, high stability and high biological value.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine processing, and relates to a preparation method of selenized polysaccharide, in particular to a preparation method of polyporus polysaccharide selenide. Background technique [0002] Polyporus, commonly known as "Polyporus flower", is widely distributed in my country. Polyporus sclerotium is one of the important traditional Chinese medicines in my country. It is flat in nature, sweet and light in taste, diuretic and damp, and can be used for dysuria, edema, diarrhea, and leukorrhea. The chemical composition contains Polyporus polysaccharide similar to Poria cocos polysaccharide, ergosterol, biotin, etc. Studies in recent years have shown that Polyporus polysaccharide is a non-specific cellular immune stimulant, which can significantly strengthen the function of phagocytes in the reticuloendothelial system and inhibit the growth of cancer cells; American scientists have done a...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 刘瑛
Owner 刘瑛
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