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Method for breaking walls of oily microalgae and extracting grease in assisted manner by virtue of saponification reaction

An auxiliary extraction and saponification reaction technology, which is applied in the direction of fat oil/fat production and fat generation, can solve the problems of destroying the original liquid components in cells, large amount of organic solvents, and low oil extraction rate, so as to improve the effect of wall breaking and oil extraction The effect of high rate and reduced dosage

Active Publication Date: 2016-01-06
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to overcome the low oil extraction rate in existing oil-containing microalgae wall breaking and oil extraction, easy to destroy the original liquid components in cells, harsh reaction conditions, large amount of organic solvents, serious environmental pollution, high energy consumption or narrow application range, etc. Disadvantages, provide a new method for breaking the wall of oil-containing microalgae and extracting its oil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] This example is used to illustrate the method of breaking the wall of oleaginous microalgae and using saponification to assist oil extraction in the present invention.

[0056] (1) Put 5 g of Nannochloropsis dry powder into a conical flask, add water to prepare a 25% by weight suspension, adjust the pH value to 8.5, and stir at 80° C. for 4 hours;

[0057] (2) Regulate the pH value to 5. In terms of Nannochloropsis dry powder, cellulase (Beijing Hongrun Baoshun Technology Co., Ltd., 2000IU / g, the same below), pectinase ( Beijing Hongrun Baoshun Technology Co., Ltd., 20000IU / g, the same below), acid protease (Beijing Hongrun Baoshun Technology Co., Ltd., 50000IU / g, the same below) and lysozyme (Sinopharm Chemical Reagent Co., Ltd., 20000IU / g , the same below), reacted at 50°C for 5h;

[0058] (3) Add sodium hydroxide to adjust the pH value to 10, stir at 70°C for 1 hour, then add acetic acid to adjust the pH value to 4, and stir at 35°C for 40 minutes;

[0059] (4) Add...

Embodiment 2

[0061] This example is used to illustrate the method of breaking the wall of oleaginous microalgae and using saponification to assist oil extraction in the present invention.

[0062] (1) Put 5g of chlorella dry powder into a conical flask, add water to prepare a 15% by weight suspension, adjust the pH value to 10, and stir at 60°C for 6h;

[0063] (2) Adjust the pH value to 4, add cellulase, pectinase, acid protease and lysozyme in an amount of 200 IU / g, and react at 40°C for 6 hours;

[0064] (3) Add potassium hydroxide to adjust the pH value to 12, stir at 60°C for 2h, then add citric acid to adjust the pH value to 3, and stir at 40°C for 20min;

[0065] (4) Add 10ml of chloroform, stir for 25min, centrifuge at 4000rpm for 15min, take the organic solvent layer containing oil above, then distill under reduced pressure at 100-200Pa at 60°C, dry at constant weight, the extraction rate of oil is shown in Table 1 .

Embodiment 3

[0067] This example is used to illustrate the method of breaking the wall of oleaginous microalgae and using saponification to assist oil extraction in the present invention.

[0068] (1) Put 5 g of Scenedesmus dry powder into a conical flask, add water to prepare a 30% by weight suspension, adjust the pH to 12, and stir at 100°C for 2 hours;

[0069] (2) Adjust the pH value to 5.5, add cellulase, pectinase, acid protease and lysozyme in an amount of 300 IU / g based on Scenedesmus dry powder, and react at 60°C for 4 hours;

[0070] (3) Add sodium hydroxide to adjust the pH value to 14, stir at 50°C for 3h, then add acetic acid to adjust the pH value to 5, and stir at 25°C for 30min;

[0071] (4) Add 50ml of petroleum ether (Beijing Chemical Plant, analytically pure), stir for 35min, centrifuge at 6000rpm for 5min, take the organic solvent layer containing oil above, then distill under reduced pressure at 100-200Pa at 50°C, and dry with constant weight. Obtain oil, and the extr...

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Abstract

The invention discloses a method for breaking walls of oily microalgae and extracting grease in an assisted manner by virtue of saponification reaction. The method comprises the following steps: (1) adjusting the pH value of a suspension of oily microalgae to 7.5-14 and carrying out hydro-thermal treatment on the oily microalgae; (2) after hydro-thermal treatment, carrying out enzymatic hydrolysis on the oily microalgae in the presence of an enzyme; (3) adjusting the pH value of the oily microalgae to be alkaline for reaction and then adjusting the pH value of the oily microalgae to be acidic for reaction; and (4) adding an organic solvent into the oily microalgae for extracting and separating grease. The method disclosed by the invention is high in grease extraction rate and mild in operating condition, can effectively maintain nutritional components of microalgae, is small in dosage of the organic solvent, environmentally-friendly and low in energy consumption, can be suitable for various microalgae such as nannochloropsis oculata, chlorella, diatom, scenedesmus, single needle algae and the like, and is wide in application range.

Description

technical field [0001] The invention relates to the technical field of microalgae bioengineering, in particular to a method for breaking the wall of oil-containing microalgae and using saponification to assist in extracting oil. Background technique [0002] Microalgae refer to tiny algae groups whose shape can only be distinguished under a microscope, and are the most important primary productivity in the biosphere. Algae has the advantages of large biomass, short production cycle, various types, high oil content, and strong environmental adaptability. It is a good raw material for biodiesel. In addition, microalgae also contains a variety of vitamins, carotene, protein, fatty acids and other components, which are the source of medicinal active substances. [0003] However, microalgae oils are wrapped in cells, and most of the bioactive substances of microalgae such as proteins, carotenoids, and microalgae polysaccharides are present in biological cells. Because microalga...

Claims

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Application Information

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IPC IPC(8): C11B1/04C11B1/10
Inventor 吴冲冲荣峻峰林伟国宗明生
Owner CHINA PETROLEUM & CHEM CORP
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