Pharmaceutical composition for treating brain gliomas and application of pharmaceutical composition

A glioma and composition technology, applied in the field of traditional Chinese medicine, can solve the problems of incomplete resection and unsatisfactory clinical chemotherapy treatment effect of glioma, and achieve good treatment, good clinical application prospects, and simple preparation Effect

Inactive Publication Date: 2016-01-13
QINGDAO JINZHI HIGH NEW TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the invasive growth of glioma, it cannot be completely removed during surgery, which determines that chemotherapy is the most important part of adjuvant therap...

Method used

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  • Pharmaceutical composition for treating brain gliomas and application of pharmaceutical composition

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: the preparation of Chinese medicine extract

[0013] Weigh 2.0kg of seaweed, 3.3kg of paparazzi, 3.3kg of ironwort, 1.4kg of xanthophyll, 1.2kg of Augusta, 1.2kg of hosta, 1.5kg of trigonum, 1.2kg of Tianzhuhuang and 1.2kg of Gastrodia elata, combine, crush, Put it in a traditional Chinese medicine extraction device, and extract it three times with an ethanol solution with a volume concentration of 75% under heat reflux, add an ethanol solution 12 times the total amount of medicinal materials for the first time, and extract for 2 hours; add an ethanol solution 11 times the total amount of medicinal materials for the second time, Extract for 1.5 hours; add ethanol solution 10 times the total amount of medicinal materials for the third time, extract for 1.5 hours; filter with four layers of gauze, combine the filtrate, concentrate under reduced pressure and recover ethanol to a thick paste, freeze-dry, crush into fine powder, pass through 100 Mesh sieve, obt...

Embodiment 2

[0014] Example 2: Culture of rat C6 glioma cells

[0015] Rat C6 glioma cells were resuscitated, and RPMI-1640 medium containing 10% inactivated fetal bovine serum (containing NaHCO 3 2g / L, penicillin 100U / mL, streptomycin 100U / mL), put the culture bottle in 5% CO 2 1. Cultivate in a constant temperature airtight cell incubator at 37°C, take 10 μL of logarithmic growth phase C6 cells, count them under a light microscope with a red blood cell counting plate, adjust the concentration so that each 10 μL contains 1×10 6 Then add 0.4% trypan blue isotonic saline solution at a ratio of 1:9, and confirm the survival rate of C6 glioma cells > 95% within 1 min.

Embodiment 3

[0016] Example 3: Study on the Curative Effect of Chinese Medicine Extracts on Brain Glioma

[0017] Healthy SD rats, 7 weeks old, male, were used for the experiment after one week of adaptive feeding. After the test started, the rat brain glioma model was first replicated, and the method was as follows: 10% chloral hydrate was used for intraperitoneal injection anesthesia, the head of the rat brain stereotaxic apparatus was fixed, the two ear needles were deep into the external auditory canal, and the hair on the top of the head was cut off. , disinfection, laying hole towels. Cut the scalp, expose the skull layer by layer, and determine the position of bregma. C6 glioma cells in logarithmic growth phase (cultivated in Example 2) were inoculated 1 mm before the midpoint of the bregma and 3.0 to 3.5 mm lateral to the right side of the sagittal suture. The inoculation site was the right caudate nucleus of the rat. Insert the needle vertically from the inoculation point. Calcu...

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Abstract

The invention discloses a pharmaceutical composition for treating brain gliomas and application of the pharmaceutical composition. The pharmaceutical composition is prepared from filtrate obtained by subjecting traditional Chinese medicinal materials to heat reflux extraction by an ethanol solution. The pharmaceutical composition comprises, by weight, 15-25 parts of sargassum pallidum (Turn.) C.Ag., 30-37 parts of vernonia patula (Ait.) Merr., 30-37 parts of cynodon dactylon (L.) Pers., 10-18 parts of airpotato yam rhizome, 9-15 parts of fiveleaf akebia fruit, 9-15 parts of flowers of fragrant plantainlily, 11-20 parts of common burreed rhizome, 9-15 parts of concretio silicea bambusae and 9-15 parts of gastrodia tuber. The pharmaceutical composition gives play to efficacy by inducing glioma cell apoptosis by multiple target points, thereby having good brain glioma treatment activity.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and in particular relates to a pharmaceutical composition for treating glioma and its application. Background technique [0002] Glioma is one of the most common tumors occurring in humans, with a high degree of progression. Generally, from the time of diagnosis, the patient's life expectancy is not more than one year. As one of the most common tumors in central system diseases, glioma accounts for about 30%-50% of adult intracranial tumors, and has the characteristics of easy recurrence and difficult treatment. Currently, the main treatment for glioma is surgery, supplemented by radiotherapy and chemotherapy. Due to the aggressive growth of glioma, it cannot be completely removed during surgery, which determines that chemotherapy is the most important part of adjuvant therapy after glioma surgery. Drug resistance leads to unsatisfactory therapeutic effects of clinical che...

Claims

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Application Information

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IPC IPC(8): A61K36/902A61P35/00
Inventor 不公告发明人
Owner QINGDAO JINZHI HIGH NEW TECH
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