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Sericin nerve conduit and its preparation method and application

A technology of sericin and nerve guide, applied in the field of medical biomaterials, can solve problems such as unsatisfactory structure and function recovery, difficult to adjust degradation performance, poor mechanical processing performance, etc., to achieve product quality control and large-scale production , promotion of migration and alignment, good mechanical properties

Active Publication Date: 2018-07-31
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the performance of this kind of material is unstable, and it is not easy to adjust its degradation performance due to the change of the host enzyme environment, and its mechanical processing performance is poor, so it is difficult to achieve large-scale production; (Using rats as an animal model, nerve damage of 10mm and below) has a good recovery effect, and for long-distance nerve damage (using rats as an animal model, nerve damage exceeding 15mm) has both structural and functional recovery. Not ideal enough to reach a level comparable to autologous nerve transplantation

Method used

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  • Sericin nerve conduit and its preparation method and application
  • Sericin nerve conduit and its preparation method and application
  • Sericin nerve conduit and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The preparation of embodiment 1 sericin nerve guide

[0063] 1. Selection of silkworm cocoons:

[0064] Silkworm cocoons of silkworm silk fibroin-deficient mutant varieties were selected (purchased from the Sericulture Research Institute of the Chinese Academy of Agricultural Sciences, and the silkworm silk fibroin-deficient mutant varieties were preserved in the National Silkworm Resource Preservation Center of the Sericulture Research Institute of the Chinese Academy of Agricultural Sciences, preservation number (or commodity number) 185Nd -s, 140Nd-s, 139Nd-s) as raw materials, the main chemical composition is: sericin.

[0065] 2. Extraction and separation of sericin

[0066] Figure 14 It is a schematic flow chart of preparing a sericin nerve guide according to an embodiment of the present invention, and the specific steps are:

[0067] 1) Weigh 1 g of cocoons of silkworm silk fibroin-deficient mutant varieties and cut them into 1 mm 2 Left and right fragments ...

Embodiment 2

[0089] Example 2 Performance analysis of sericin nerve guide

[0090] The following performance analysis is carried out to the sericin nerve guide (sample D1) prepared in embodiment 1, specifically:

[0091] 1. Macro structure

[0092] Using the selected mold, the obtained sericin nerve conduit was prepared into a hollow conduit structure with an inner diameter of 1.5 mm, a wall thickness of 0.75 mm, and a length of 20 mm.

[0093] 2. Microstructure.

[0094] Sericin nerve conduits were observed under a scanning electron microscope (ULTRA PLUS-43-13, Zeiss, Germany). like Figure 5 As shown, A and B are SEM images of the cross-section of the sericin nerve conduit, the scales are 500 microns and 100 microns respectively, the interior of the conduit is a multi-void structure with an average pore size of 42.24 μm, which can well support cell growth and promote exchange of nutrients and metabolites; Figure 5 Among them, C and D are the SEM images of the surface of the serici...

Embodiment 3

[0108] Example 3 Evaluation of Sericin Supporting Nerve Cell Survival and Proliferation Ability

[0109] Experimental group: use the aseptic sericin protein aqueous solution extracted in Example 1 to directly add to the cell well plate, freeze at -80°C for 4 hours, and then freeze-dry in a vacuum freeze dryer to obtain freeze-dried sericin Protein scaffold: Rat Schwann cells (RSC96) collected from cell culture flasks were suspended, blown apart, and planted on cell culture plates covered with freeze-dried sericin scaffolds. The medium used for cell culture was DMEM (Dulbecco's Modified Eagle's Medium) medium, and the cells were placed in a cell culture incubator (37°C, CO 2 The concentration is 5%, and the humidity is 100%).

[0110] In the control group, the cells were directly planted on ordinary well plates.

[0111] like Figure 8 As shown, after planting, the CCK-8 method was used to detect the proliferation of cells 1, 3, 5 and 7 days after planting. Depend on Figu...

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Abstract

The invention provides a sericin nerve guide, its preparation method and application. The sericin nerve conduit component provided by the present invention is sericin, and its preparation method comprises: using silkworm cocoons of a silk fibroin-deficient mutant variety Bombyx mori as raw material, pulverizing it and sterilizing it with ultraviolet light or ethanol, and then using LiBr or LiCl aqueous solution Carry out the extraction of sericin protein, and make the sericin protein aqueous solution with the concentration of 0.5~20wt% from the extract; For semi-finished catheters, the amount of covalent cross-linking agent used is to add 2-500 μL of covalent cross-linking agent with a concentration of 0.1-50 wt% per 1 ml of sericin aqueous solution; freeze-dry the semi-finished sericin nerve catheter to obtain sericin nerve guide. The sericin nerve conduit provided by the invention has good biological activity and biocompatibility, can be naturally degraded in vivo and can promote regeneration of long-distance injured peripheral nerves.

Description

technical field [0001] The invention relates to a sericin nerve guide and its preparation method and application, belonging to the field of medical biomaterials. Background technique [0002] Peripheral nerve injury is a relatively common disease, usually caused by trauma caused by traffic accidents, natural disasters, wars, tumor resection, congenital deformities, etc. About 2.8% of traumatic diseases can lead to peripheral nerve injury, resulting in nerve rupture. Peripheral nerve injury usually leads to sensory and motor dysfunction of patients, high disability rate, seriously affects the quality of life of patients, and brings huge economic burden to families and society. [0003] The principle of treatment for peripheral nerve injury is to restore the continuity of the nerve as early as possible. The current treatment methods mainly include autologous nerve transplantation, allogeneic nerve transplantation, tension-free direct suture of the broken ends, and tissue engin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22
Inventor 王琳王征谢洪建杨文罗震王健刘佳
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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