A kind of neural unit spherical support and its preparation

A nerve unit and spherical technology, applied in medical science, prosthesis, etc., can solve the problems of not being able to meet the requirements of repair materials in ideal nerve defect areas at the same time, and the three-dimensional scaffold cannot be applied to central nerve defect areas, etc., to achieve rapid recovery of nerve function and promote The effect of quick refactoring

Active Publication Date: 2018-04-03
药谷(温州)科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These specially constructed three-dimensional scaffolds cannot be applied to the central nervous system defect area, and have their own disadvantages
For the central nerve defect area caused by intracranial injury, there is still a lack of effective repair materials, and none of the existing materials or strategies can meet the requirements of the ideal repair material for the nerve defect area at the same time, that is, it has good mechanical properties and spatial structure. The optimal physical, chemical and biological microenvironment of stem cells promotes their growth, induces the directional differentiation of nerves for a long time, and promotes the rapid construction of neural units in the nerve defect area and the effective formation of neural networks

Method used

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  • A kind of neural unit spherical support and its preparation
  • A kind of neural unit spherical support and its preparation
  • A kind of neural unit spherical support and its preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 The blank spherical stent prepared by natural polymer material chitosan

[0025] a. Chitosan spherical scaffold

[0026] Get 60mg chitosan (molecular weight 10,000), dissolve in 4ml 3% acetic acid, completely dissolve into a uniform solution. Add 1ml of acetone to it, ultrasonically disperse to form an O / W emulsion, add it to 40ml of liquid paraffin containing 50mg of Span 80: petroleum ether (volume ratio is 1:1), stir and mix at 400 rpm to form O / W / O re-emulsion, drop 0.5ml of glutaraldehyde solution for cross-linking and solidification, continue to stir for 2 hours, centrifuge to separate the microspheres, wash three times with petroleum ether, and naturally evaporate to dry to form chitosan blank hollow microspheres. The surface of chitosan blank microspheres was concentrically punctured with microneedles with a diameter of 100 μm to form a frontal chitosan spherical scaffold with 100 μm pores on the surface and a diameter of 2000 μm.

[0027] The follo...

Embodiment 2

[0031] Example 2 Blank spherical stent prepared from the semi-synthetic polymer material ethyl cellulose

[0032] a. Ethylcellulose spherical scaffold

[0033] Polyvinylpyrrolidone (PVP) and ethylcellulose (EC) were dissolved in 20ml of absolute ethanol, and it was prepared as a mixed solution containing 3.6% polyvinylpyrrolidone and 33% ethylcellulose by mass percent, ultrasonic After uniform dispersion, 4ml of ether was added at 20°C as a dispersed phase solution. Separately prepare 200ml of liquid paraffin containing 50mg of Span 80, slowly add it into the dispersed phase solution at 20°C, stir at 400 rpm, evaporate the organic solvent under ventilated conditions, collect the microspheres by filtration, and dissolve them with n-hexane Washing and vacuum drying to obtain blank hollow microspheres, the surface of which is subjected to concentric perforation with a laser beam to form ethylcellulose spherical scaffolds with 50 μm channels on the surface and a diameter of 1000 ...

Embodiment 3

[0038] Example 3 Blank spherical stent prepared by artificially synthesizing polymer material polyvinyl alcohol

[0039] a. Polyvinyl alcohol spherical bracket

[0040]Prepare 20ml of polyvinyl alcohol (PVA) aqueous solution with a mass percentage of 2%, add 20mg of Span 80, dissolve and mix, add it to 60ml of n-hexane, perform high-speed homogenization to form a W / O emulsion, and add borax saturated aqueous solution drop by drop 20ml, stirred at 300rpm for 3h, separated microspheres after 2h at low temperature, washed 3 times with absolute ethanol, dried in vacuum at 40°C, and the surface was treated with concentric perforation with laser beams to form polyvinyl alcohol spherical scaffolds with 40μn micropores on the surface and a diameter of 800μm .

[0041] The following experimental control groups were prepared in a similar manner:

[0042] b. Non-perforated group: that is, the non-perforated group of polyvinyl alcohol spherical stents obtained without laser beam treatme...

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Abstract

The invention provides the composition, preparation and application of a nerve unit spherical support. A large number of pores are distributed in the nerve unit spherical stent of the present invention, and these pores present a spatial structure radiating from the center to the surroundings. The central hole communicates with all the pores. The diameter of the nerve unit spherical stent is 500-2000 μm, and the diameter of the internal pores is 20-100 μm. The tract contains stem cells and vascular endothelial cells. After the nerve unit spherical stent of the present invention is transplanted, it can achieve multiple purposes such as effective filling of the nerve defect area, rapid reconstruction of the local neural network and microvessels, and achieve the effect of rapid recovery of the function of the nerve defect area.

Description

technical field [0001] The present invention relates to providing a nerve unit spherical support, in particular to the composition, preparation and application of a nerve unit spherical support for rapid filling of nerve defect areas and rapid repair of neurons. Background technique [0002] At present, due to traffic accidents, geological disasters, wars, etc., the number of patients with intracranial injuries is increasing. After clinical operations, there are usually large intracranial defect areas. If they cannot be healed effectively, they will cause barriers to mobility, language, memory or intelligence. . [0003] The clinical application of collagen sponge as an intracranial filling material can reduce the leakage of cerebrospinal fluid, fill the residual intracranial cavity and reconstruct the skull base. Some people also use hydrogel as a filler for the postoperative intracranial residual cavity. However, whether collagen sponge or hydrogel, it only plays a tempo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/50A61L27/38A61L27/54A61L27/56
Inventor 赵应征鲁翠涛肖健虞希冲徐荷林杨伟张宏宇杨靖靖许洁范子梁陈翩翩
Owner 药谷(温州)科技发展有限公司
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