Method for preparing 1-deoxynojirimycin from mulberry twigs through solid fermentation

Abstract

The invention discloses a method for preparing 1-deoxynojirimycin (1-DNJ) from mulberry twigs through solid fermentation, and belongs to the field of medicines and healthcare products. According to the method, a culture medium, which is mainly composed of mulberry twigs, is adopted; bacillus subtilis 20632 is taken as the fermentation strain to carry out solid fermentation; wherein the mulberry twig solid fermentation conditions are as follows: pH value is 6.6-9.2; water, which is used to wet mulberry twigs, accounts for 40 to 65% of the total weight of mulberry twigs; 1.5 % of lactose and 1.5 % of peptone are added into the wetting liquid and taken as the carbon source; and the solid fermentation lasts for 2 to 5 days at a temperature of 37 DEG C. After fermentation, the fermentation product is subjected to extraction, membrane filtration, condensation, and drying so as to obtain high quality 1-DNJ. The solid fermentation method has the advantages of high yield, high purity product, low cost, and suitability for large scale industrial production.

Description

technical field [0001] The invention relates to a method for preparing 1-deoxynojirimycin (1-DNJ) by solid-state fermentation using mulberry branches as raw materials, belonging to the field of medicines and health care products. Background technique [0002] 1-deoxynojirimycin (1-deoxynojirimycin, DNJ) is a polyhydroxypiperidine alkaloid present in mulberry and other plants and microorganisms, the chemical name is 3,4,5-trihydroxy-2-hydroxymethyl Tetrahydropyridine, the molecular formula is C 6 h 13 o 4 , with a molecular weight of 163. [0003] 1-DNJ has an inhibitory effect on α-glucosidase, is an effective ingredient for lowering blood sugar and treating diabetes, and also has pharmacological activities such as anti-virus and anti-tumor metastasis. [0004] 1-DNJ is derived from natural products, biosynthesis, and chemical synthesis, and its preparation methods mainly include the following: [0005] One is extracted from plants. There is a small amount of 1-deoxyno...

AI Technical Summary

Problems solved by technology

There is a small amount of 1-deoxynojirimycin (less than 0.1%) in some microorganisms of mulberry trees. This process is used to prepare 1-DNJ. Because 1-DNJ has poor thermal stability and low product content, the practical significance is not great.

The disclosed method of CN10134586A is to take the crude extract of mulberry leaves as raw material (containing more than 40% of DNJ), after dissolving in water, directly pass through cationic resin, then use eluent to elute and collect step by step, and finally obtain the product with relatively high purity through repeated recrystallization. High product, because the mixed solvent used in recrystallization is not easy to separate and recycle, so the production cost is high

The method disclosed in CN101671294A not only separates and obtains the DNJ product, but also obtains another product, mulberry leaf flavonoids, from mulberry leaves. Specifically, dry mulberry leaves are used as raw materials, extracted with ethanol, centrifugally filtered, and flocculated and precipitated by adding a flocculant; The filtrate is sprayed through the macroporous adsorption resin and cationic resin in turn to obtain the DNJ product. Due to the long process and the use of flocculation and sedimentation, the loss of this method is relatively large, and the final yield is also low. This is the method. biggest shortcoming