Method for detecting three fat-soluble vitamins A, E and K1 in milk powder

A fat-soluble vitamin and detection method technology, which can be used in measuring devices, instruments, scientific instruments, etc., can solve the problems of large solvent consumption, complicated operation, unstable vitamins, etc.

Active Publication Date: 2016-03-02
厦门佰能检验技术服务有限公司
View PDF4 Cites 17 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These fat-soluble vitamins are unstable in the external environment and are easily oxidized and destroyed by light, oxygen, etc.
At present, the determination method of fat-soluble vitamins in formula milk powder is usually: after the sample is processed by enzymolysis and saponification, it is extracted with a non-polar organic solvent (commonly used petroleum ether, n-hexane, ether, etc.) liquid-liquid extraction, and then reversed-phase chromatography

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting three fat-soluble vitamins A, E and K1 in milk powder
  • Method for detecting three fat-soluble vitamins A, E and K1 in milk powder
  • Method for detecting three fat-soluble vitamins A, E and K1 in milk powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1: Fat-soluble vitamins A, E, and K in milk powder 1 detection

[0055] 1. Sample preparation

[0056] Weigh about 1g of sample (infant formula milk powder of a commercially available brand) into a 10mL colorimetric tube, add 45°C warm water to dissolve to 10mL, divide 1mL into a 20mL centrifuge tube, add 5mL of absolute ethanol to precipitate protein, add 3mL Extract with n-hexane, add 5mL saturated sodium chloride solution to inhibit emulsification, mix well and centrifuge at 5000rpm at a high speed, take the n-hexane layer and dry it with nitrogen, dissolve it with methanol and dilute to 1mL, filter through a 0.22μm filter membrane, and supply the liquid phase Chromatography-tandem mass spectrometry.

[0057] 2. Preparation of standard solution

[0058] Vitamin A (retinol, purity 95.5%), vitamin A acetate (retinyl acetate, purity 98.8%), vitamin A palmitate (retinyl palmitate, purity 92.5%), vitamin E (DL -α-tocopherol, purity 95.8%), vitamin E acetate (D...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for detecting three fat-soluble vitamins A, E and K1 in milk powder. The method comprises the following steps: weighing a sample, adding water to dissolve the sample and making a constant volume, placing part of the sample solution obtained after making the constant volume into a centrifugal tube, adding anhydrous ethanol, n-hexane and a saturated sodium chloride solution, uniformly mixing, centrifuging and layering, drying the n-hexane layer with nitrogen, dissolving with methanol to make a constant volume, detecting the dissolved solution and a standard work solution by a liquid chromatography-tandem mass spectrometer, respectively carrying out sample introduction to obtain a working curve with concentrations of vitamin A, vitamin A acetate, vitamin A plamitate, vitamin E, vitamin E acetate and vitamin K1 in the standard solution being the horizontal ordinate and with peak area of the six vitamins being the vertical coordinate; carrying out quantitative calculation on quantitative ion by the use of the working curve to obtain concentrations of the six vitamins in the sample solution to be measured; and obtaining contents of vitamin A, vitamin E and vitamin K1 by conversion. The method of the invention is simple, rapid and accurate, and has high sensitivity.

Description

technical field [0001] The invention relates to a method for detecting fat-soluble vitamins, in particular to three kinds of fat-soluble vitamins A, E and K in milk powder 1 detection method. Background technique [0002] Fat-soluble vitamins A, E, K 1 Often added to formula as a nutritional fortifier. Vitamin A, also known as retinol, has the main physiological functions of maintaining vision and bone health, participating in cell division and cell recognition, participating in growth, reproduction and maintaining the integrity of the immune system. Vitamin E, also known as tocopherol, has 8 types in nature: α-, β-, γ-, δ-tocopherol and α-, β-, γ-, δ-tocotrienol, among which α-tocopherol The strongest biological activity. Vitamin K 1 Also known as phytonadione, it controls blood coagulation and is an essential substance for the synthesis of coagulation proteins in the liver. These fat-soluble vitamins are unstable in the external environment and are easily oxidized an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/88
Inventor 严丽娟林立毅吴敏徐敦明张洁陈鹭平张志刚黄志强
Owner 厦门佰能检验技术服务有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap