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Preparation method and application of photoelectrochemical aptamer sensing electrode

A photoelectrochemical and sensing electrode technology, applied in the direction of material electrochemical variables, etc., can solve the problems of low selectivity and sensitivity, long response time, complex instruments, etc., and achieve the effect of low detection cost, high sensitivity, and simple preparation process

Inactive Publication Date: 2016-03-16
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Aiming at the shortcomings of traditional detection methods such as complex instruments, long response time, low selectivity and sensitivity, the purpose of the present invention is to provide a fast and highly sensitive MC-LR photoelectrochemical sensing electrode preparation method, and the photoelectrochemical Sensing electrodes applied to the detection of MC-LR

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  • Preparation method and application of photoelectrochemical aptamer sensing electrode
  • Preparation method and application of photoelectrochemical aptamer sensing electrode

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Experimental program
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Embodiment 1

[0031] (1) The preparation method of BiOBr-NG nanocomposites is as follows: 0.12g bismuth nitrate pentahydrate (Bi(NO 3 ) 3 ·5H 2 O) dissolved in deionized water, with nitric acid (HNO 3 ) solution to adjust its pH value to 3. In addition, 0.8mg NG was dispersed in CTAB solution and mixed evenly by ultrasonic. Then Bi(NO 3 ) 3 ·5H 2 The O solution was slowly dropped into the mixed solution of NG and CTAB. The above mixture was transferred to a round bottom flask and reacted in an oil bath at 60°C for 3h. After the reaction was completed, the prepared precipitate was repeatedly washed three times with ethanol and deionized water, and dried at 80°C for future use.

[0032] Wherein, the volume of the solvent deionized water used is 50mL, CTAB and Bi(NO 3 ) 3 ·5H 2 The molar ratio of O was 1:1, the ultrasonic mixing time was 0.5 h, and the concentration of CTAB was 8×10 -3 mol / L.

[0033] (2) Surface pretreatment of ITO electrode: Boil the ITO electrode with 1M sodium ...

Embodiment 2

[0037](1) The preparation method of BiOBr-NG nanocomposites is as follows: 0.12gBi(NO 3 ) 3 ·5H 2 O dissolved in deionized water, with nitric acid (HNO 3 ) solution to adjust its pH value to 3. In addition, 1.56 mg was dispersed in CTAB solution and ultrasonically mixed. Then Bi(NO 3 ) 3 ·5H 2 The O solution was slowly dropped into the mixed solution of NG and CTAB. The above mixture was transferred to a round bottom flask and reacted in an oil bath at 80°C for 3h. After the reaction was completed, the prepared precipitate was repeatedly washed three times with ethanol and deionized water, and dried at 80°C for later use. figure 1 is the transmission electron microscope image of the synthesized BiOBr-NG nanocomposite, by figure 1 It can be seen that the flaky BiOBr is dispersed on the NG graphene sheet.

[0038] Wherein, the volume of the solvent deionized water used is 50mL, CTAB and Bi(NO 3 ) 3 ·5H 2 The molar ratio of O was 1:1, the ultrasonic mixing time was 0....

Embodiment 3

[0043] (1) The preparation method of BiOBr-NG nanocomposites is as follows: 0.12gBi(NO 3 ) 3 ·5H 2 O dissolved in deionized water, with nitric acid (HNO 3 ) solution to adjust its pH value to 3, in addition, 4.5mg NG was dispersed in CTAB solution and ultrasonically mixed. Then Bi(NO 3 ) 3 ·5H 2 The O solution was slowly dropped into the mixed solution of NG and CTAB. The above mixture was transferred to a round bottom flask and reacted in an oil bath at 120°C for 3h. After the reaction was completed, the prepared precipitate was repeatedly washed three times with ethanol and deionized water, and dried at 80°C for future use.

[0044] Wherein, the volume of the solvent deionized water used is 50mL, CTAB and Bi(NO 3 ) 3 ·5H 2 The molar ratio of O was 1:1, NG, ultrasonic mixing time was 0.5h, and the concentration of CTAB was (8×10 -3 mol / L).

[0045] (2) Surface pretreatment of ITO electrode: Boil the ITO electrode with 1M sodium hydroxide for 15-20 minutes, then ul...

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Abstract

The invention belongs to the field of photoelectrochemical sensing, and relates to a preparation method of a photoelectrochemical aptamer sensing electrode and an application of the photoelectrochemical aptamer sensing electrode to detection of microcystins. The method comprises steps as follows: firstly, BiOBr-NG nano-composites are prepared with a wet chemical method; then ITO (indium tin oxide) electrode surfaces are modified with the BiOBr-NG nano-composites, the pi-pi stacking action of NG and aptamers is further utilized for fixation of the aptamers and construction of a photoelectrochemical platform, and then the photoelectrochemical platform is applied to detection of the microcystins. The invention aims to invent the preparation method of the photoelectrochemical aptamer sensing electrode which adopts the simple preparation technology and has the good selectivity, the high sensitivity and the low detection cost.

Description

Technical field [0001] The present invention belongs to the field of photoelectric chemical sensing, involving a preparation method for photoelectric chemical adaptive sensor electrodes, especially involved a modifiers on the surface of the oxidation tin (commonly known as ITO) electrode surface-NG) Based on the nano-complex, further use the nitrogen miscellaneous graphene and nucleic acid adaptation to fix the preparation method of the sensor of the optoelectronic chemical detection platform. Background technique [0002] The increasingly severe phenomenon of water and nutritionalization of water bodies has become a global environmental problem.The main reason for the formation of Shuihua is due to the excessive reproduction of algae in the water body. The cyanobacteriaxins produced by the poisonous cyanobacteria are accumulated in water, which seriously threatens people's drinking water safety.Candida toxin is a cycloid peptide composed of seven amino acids, which are generally...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30
Inventor 杜晓娇王坤蒋鼎钱静
Owner JIANGSU UNIV
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