Unlock instant, AI-driven research and patent intelligence for your innovation.

Pegylated recombined candida utilis urate oxidase freeze-drying injection

A Candida utilis, PEGylation technology, applied in the direction of oxidoreductase, freeze-dried transportation, enzymes, etc., can solve the problems of activity decline and loss

Active Publication Date: 2016-03-23
SHENYANG SUNSHINE PHARMA
View PDF11 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Due to the complexity of the structure of PEGylated recombinant Candida utilis uric acid oxidase, the three stages of freeze-drying (low temperature effect, freezing effect and dehydration effect) may lead to a decrease or loss of activity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pegylated recombined candida utilis urate oxidase freeze-drying injection
  • Pegylated recombined candida utilis urate oxidase freeze-drying injection
  • Pegylated recombined candida utilis urate oxidase freeze-drying injection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Effects of Polyethylene Glycol Modification Products with Different Molecular Weights on Enzyme Activity

[0040] The polyethylene glycol linking group was selected as succinimidyl succinate, abbreviated as mSS-PEG. Choose mSS-PEG with molecular weights of 5K, 20K, and 30K to modify the purified recombinant urate oxidase protein. The pH of the modification reaction is controlled at 7.5-8.0. The buffer system is 100mM phosphate buffer. The recombinant urate oxidase protein and mSS -The reaction molar ratio of PEG is 1:30, and the reaction is allowed to stand at room temperature for 120 minutes. After the reaction was finished, an ultrafiltration centrifuge tube with a molecular weight cut-off of 30KD was used to perform ultrafiltration and centrifugation for 10 times to obtain purified PEGylated recombinant urate oxidases of different molecular weights ( figure 1 ). The enzymatic activities of products before modification and after modification with different molecular...

Embodiment 2

[0045] Immunogenicity experiment of polyethylene glycol modified products with different molecular weights

[0046] 70 SPF mice. They were randomly divided into 3 groups, 20 rats in each group, and blank serum was taken from the other 10 rats as blank control. By subcutaneous injection, three groups of mice were given recombinant Candida urate oxidase, recombinant Candida urate oxidase-PEG-5K and recombinant Candida urate oxidase-PEG-20K respectively, with a dosage of 1.54 mg / kg, administered twice a week for 5 consecutive weeks. Before the administration, blood was collected from 10 blank mice as a blank control; blood was collected from each mouse at the 6th and 8th weeks, and the serum anti-uric acid oxidase antibody concentration was detected by ELISA method. The volume of each blood collection is not less than 250ul. The experimental results are shown in Table 2.

[0047] Table 2 ELIAS method to detect serum anti-uric acid oxidase antibody titer

[0048]

[0049]...

Embodiment 3

[0051] The isoelectric point of the pegylated recombinant urate oxidase is detected, and a suitable pH value range of the formulation is selected.

[0052] Capillary isoelectric focusing is an ideal method to obtain the actual isoelectric point of protein, so capillary isoelectric focusing is used to detect the isoelectric point of PEGylated recombinant urate oxidase. Since recombinant urate oxidase undergoes PEGylation, the interference of polyethylene glycol on isoelectric detection is very serious, so only the isoelectric point of recombinant urease can be detected, and the test results are shown in figure 2 . pass figure 2 The results showed that the isoelectric point of recombinant urate oxidase is about 7.3, because the binding site of recombinant urate oxidase and polyethylene glycol is mainly lysine, which is a basic amino acid, so recombinant urate oxidase and polyethylene glycol After ethylene glycol is combined, the isoelectric point shifts toward acidity. There...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention provides a pegylated recombined candida utilis urate oxidase freeze-drying injection for treating various diseases caused when blood uric acid in a human body rises. The pegylated recombined candida utilis urate oxidase freeze-drying injection is characterized by being composed of pegylated recombined candida utilis urate oxidase as active components, a freeze-drying protecting agent for keeping the activity of protein and keeping protein stable in the freezing or freeze-drying process, a filling agent, an acid and alkaline adjusting agent and buffering liquid for maintaining a certain pH value condition. The injection has the advantages that the complete spatial configuration and bioactivity of the freeze-dried pegylated recombined candida utilis urate oxidase, preservation of medicine is facilitated, and the medicine can be re-dissolved and renatured easily in a short time.

Description

technical field [0001] The invention relates to a new freeze-dried preparation of biological products, a preparation method and application in related therapeutic fields. Specifically, the invention relates to the administration of PEGylated recombinant products for various diseases caused by elevated blood uric acid in the human body. Candida prion urate oxidase lyophilized injection. Background technique [0002] Uric acid is the end product of purine metabolism in birds, reptiles and primates including humans, because in these animals there is a lack of uric acid that uses molecular oxygen as the receptor to catalyze the further decomposition of uric acid into allantoin, carbon dioxide and hydrogen peroxide oxidase. Uric acid is excreted by the kidneys as the end product of purine metabolism in the human body. When uric acid production exceeds renal metabolism, such as excessive intake of purine-rich foods, increased purine metabolism caused by leukemia and lymphoma and ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K47/48A61K38/44A61K9/19A61P19/06A61K47/60
CPCA61K9/0019A61K9/19A61K38/44C12Y107/03003
Inventor 娄竞袁杰徐秀玲靳征
Owner SHENYANG SUNSHINE PHARMA
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com