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Continuous ion exchange extraction technology of L-arginine

A continuous ion extraction process technology, applied in the preparation of organic compounds, chemical instruments and methods, organic chemistry, etc., can solve the problems affecting the product purification process, large amount of eluent and water consumption, and low clarity of feed liquid, etc. Achieve the effect of less impurities, stable product quality and easy elution

Active Publication Date: 2016-03-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the above method, the clarity of the feed liquid obtained by flocculation and sterilization is not high, and the residual flocculant may cause the blockage of the moving bed, which affects the downstream product purification process; while using the traditional fixed bed for ion exchange, the product yield is low, The resin utilization rate is not high, and the amount of eluent and water is large, and the product quality is unstable

Method used

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  • Continuous ion exchange extraction technology of L-arginine

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Effect test

Embodiment 1

[0021] 1) Fermentation liquid pretreatment: the fermentation liquid whose L-arginine concentration is 56g / L enters the ceramic membrane filtration system to remove bacterial cells, and the filtrate enters the ultrafiltration system to remove the protein in the fermentation liquid subsequently, and the gained filtrate is adjusted with hydrochloric acid. pH to 5-6.5.

[0022] 2) Continuous moving bed impurity removal: pump the pretreated feed liquid into the continuous moving bed for impurity removal. In the continuous moving bed system, a column with a diameter of 2.5 cm and a length of 30 cm is used. The walking time of the separation unit is 16 minutes. The feed liquid enters the system through the 6#, 7# and 8# columns, and the feed flow rate is 3BV / h. After the primary adsorption, the feed liquid enters 9-11# in series for secondary adsorption; after the adsorption, the 1-3# columns are washed in series with water , the washing flow rate is 1BV / h, the washing liquid enters...

Embodiment 2

[0025] 1) Fermentation liquid pretreatment: the fermentation liquid whose L-arginine concentration is 51g / L enters the ceramic membrane filtration system to remove the bacterial cells, and the filtrate enters the ultrafiltration system to remove the protein in the fermentation liquid subsequently, and the gained filtrate is adjusted with hydrochloric acid. pH to 5-6.5.

[0026] 2) Continuous moving bed impurity removal: the ultrafiltration membrane filtrate is pumped into the continuous moving bed for impurity removal. In the continuous moving bed system, a column with a diameter of 2.5 cm and a length of 30 cm is used, each of which is filled with 100 g resin, and the turntable period is 6 h, that is, each separation unit Walking time 16min. The feed liquid enters the system through the 6#, 7# and 8# columns, and the feed flow rate is 3BV / h. After the primary adsorption, the feed liquid enters 9-11# in series for secondary adsorption; after the adsorption, the 1-3# columns ar...

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Abstract

The invention provides an extraction technology of L-arginine which is prepared through microbial fermentation. The extraction technology comprises the following steps that 1, L-arginine fermentation liquor is filtered through a ceramic membrane to remove bacteria and then filtered through an ultrafiltration membrane to remove protein; 2, membrane filtrate enters a continuous ion exchange system and is adsorbed through cation exchange resin and eluted through ammonia water, and eluent of the L-arginine is obtained; 3, ammonia removing, decoloration, concentration crystallization, centrifugal separation and drying are conducted after continuous ion exchange is conducted, and an L-arginine finished product is obtained. The total recovery of the L-arginine is 80 percent or above, and the product purity is 95 percent or above.

Description

technical field [0001] The invention belongs to the technical field of L-arginine production, in particular to a method for separating and purifying L-arginine from fermentation broth. Background technique [0002] L-arginine is a basic amino acid containing a guanidine group. The pure product is a white crystalline powder. It exists widely in various organisms. It plays an important role in the growth, development and metabolism of humans and animals. It is a protein in the human body. It is the raw material for the synthesis of creatine and creatine, and is also an important intermediate metabolite in the urea cycle of the organism. It is a non-essential amino acid for healthy adults, but its synthesis is slow in the body; it is an essential amino acid for infants and young children. Therefore, it is a semi-essential amino acid. amino acid. L-arginine not only participates in the synthesis of hormones and physiologically active substances in the human body, but also parti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C277/08C07C279/14
CPCC07C277/08C07C279/14
Inventor 饶志明许正宏徐美娟黄根树杨套伟张显
Owner JIANGNAN UNIV